Home | Community | Message Board

Magic Mushrooms Zamnesia
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Next >  [ show all ]
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,794
Loc: Milky way
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27016137 - 11/01/20 07:54 PM (1 year, 2 months ago)

Quote:

Mateah said:
Practically every type of bacteria that's in our home/substrates divide every 20-30min on average in good conditions. They look wimpy as hell on agar but in liquids they talk a whole lot of smack, really fast as well and they back that shit up. Reinforcements are always right around the corner and you're already fucked
:itseveryone:



There's an unfathomable amount of bacteria out there. Even in beer there's a few dozen common contaminants let alone rare ones. And beer is a environment with a pH that's rough, hop acids that are antibacterial to most bacteria, co2, no air, pressure, yeast has some defenses, and low leftover nutrition.
Some bacteria may only grow to a few hundred cells per mL which is hard to detect with all but PCR


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

BTC: 1G7roCpYmNp4VqnKySQeBkaFTc7JMzKLpL
ETH: 0x2091cE981af8129986C50AC0818e1aCA76076768
DOGE: DQNyGtUNHwYUrGECag1VRmMcf6QVeTY4V1


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinesonoramo
Contaminant
Male User Gallery


Registered: 02/27/19
Posts: 544
Loc: California, baby! Flag
Last seen: 23 hours, 28 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: bodhisatta]
    #27042223 - 11/16/20 03:14 PM (1 year, 2 months ago)

Quote:

bodhisatta said:
There's an unfathomable amount of bacteria out there. ... Some bacteria may only grow to a few hundred cells per mL which is hard to detect with all but PCR




So,... looking for bacteria in the LC with a microscope isn't likely to help?

Would it be a worthwhile "community project" to collect contaminant reports from LC rejects? It would be great if we knew what contaminants really are most common across different locations. For example, I dumped one LC yesterday because a test plate grew trichoderma. It took 3 days to sporulate in the petri, alongside the mycelium.

I've never found bacteria in my LC test plates, but maybe I just don't know how to look for them. When I make a test plate, I try to catch just clumps of growth after spinning the stir bar as fast and hard as I can make it go. Is that the way to get a representative sample? How large a sample do you inject onto the agar? Are there dyes or other ways to make bacteria more visible in the test plate? Maybe I should be testing pH as well?


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,147
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: sonoramo]
    #27042539 - 11/16/20 06:58 PM (1 year, 2 months ago)

Quote:

sonoramo said:
Would it be a worthwhile "community project" to collect contaminant reports from LC rejects?



That would be a very worthwhile project in my opinion! I encourage everyone to post their LC's in this thread so we have a variety of different LC 'situations' documented to easier map all the visual indications!

Quote:

I've never found bacteria in my LC test plates, but maybe I just don't know how to look for them.



That's because the standard agar recipe of 2% agar isn't very suitable for detecting bacterial growth. The vast majority of bacteria requires a liquid environment to grow and unless the bacteria is very motile they would in addition need moving water to be able to spread their colonies since most baciera are non motile.  That's why microbiologists use mostly liquid and  sometimes semi-solid media's like motility agar for propagation. Even tho bacteria have the ability to deal with differences in osmotic pressure, some microbes are better at this some are do not handle lower osmolarity all that well. All in all bacteria as an organism no matter of strain all require extremely high water activity rates compared to for example myc or mold, so the wetter the environment the more suitable it is for bacterial growth. Hope that helps!


BTW you have any LC projects going on right now?


--------------------
Water Tub


Edited by Mateja (11/16/20 07:01 PM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinesonoramo
Contaminant
Male User Gallery


Registered: 02/27/19
Posts: 544
Loc: California, baby! Flag
Last seen: 23 hours, 28 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27042575 - 11/16/20 07:21 PM (1 year, 2 months ago)

Quote:

Mateah said:
That's because the standard agar recipe of 2% agar isn't very suitable for detecting bacterial growth. The vast majority of bacteria requires a liquid environment to grow and unless the bacteria is very motile they would in addition need moving water to be able to spread their colonies since most baciera are non motile.  That's why microbiologists use mostly liquid and  sometimes semi-solid media's like motility agar for propagation. Even tho bacteria have the ability to deal with differences in osmotic pressure, some microbes are better at this some are do not handle lower osmolarity all that well. All in all bacteria as an organism no matter of strain all require extremely high water activity rates compared to for example myc or mold, so the wetter the environment the more suitable it is for bacterial growth. Hope that helps!





Thanks. That is helpful. Most of my experience with bacterial colonies is on food in the refrigerator. Eventually they get big enough to see (and smell!) easily. I'll take a "bottom line" that maybe it isn't so bad if the extra liquid from inoculating a test plate softens the agar.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinesonoramo
Contaminant
Male User Gallery


Registered: 02/27/19
Posts: 544
Loc: California, baby! Flag
Last seen: 23 hours, 28 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27042587 - 11/16/20 07:30 PM (1 year, 2 months ago)

Quote:


BTW you have any LC projects going on right now?




I tried to answer by PM so the rest of the community doesn't get sidetracked, but your account doesn't accept PMs. So,...

Yes:

  • GT's (now fruiting and so done with it, but saving LC in the refrigerator)
  • Unknown cubes from deep in my spore-print collection but they are old friends :wink:
  • Cambodians, almost ready to inoculate
  • Quilla, very ready, will probably inoculate this week
  • Oyster culture that's previously "worked" from agar and cardboard, now ready with LC
  • Tarragon oyster from commercial syringe, trying to expand before inoculating


In all cases (other than the commercial syringe) it's taken some effort to go from spores to agar to LC, but I seem to get better at it as I go.

My current interest is growing oysters in larger quantities. I just can't consume cubes as fast as I could grow them now that I have a working LC (or four...).

I'm using 16ga dispensing (blunt) needles to sample from agar culture into squat 4oz mason jars. If a culture seems to be clean, then move up to a pint mason jar. Finally, inoculate oats with 20ml of LC, and I've seen full colonization in about 1.5 to 2 weeks. In all cases, lids with SHIP and Tyvek filters. Newer lids I've used two layers of Tyvek in case the broth splashes.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,147
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: sonoramo]
    #27042630 - 11/16/20 07:55 PM (1 year, 2 months ago)

My account doesn't accept PM's? You mean the automatic message said to "ask cultivation related questions in the public forum"? I guess it's aight to send me links or pics if you want but in general it's much more productive for everyone if we conduct research here instead of PM's :super:


Do you have pics of any of the LC's you have going right now?


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinesonoramo
Contaminant
Male User Gallery


Registered: 02/27/19
Posts: 544
Loc: California, baby! Flag
Last seen: 23 hours, 28 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27042744 - 11/16/20 09:05 PM (1 year, 2 months ago)

Quote:

Mateah said:
My account doesn't accept PM's? You mean the automatic message said to "ask cultivation related questions in the public forum"?...





More like a message that said "this user doesn't accept PM's."

Quote:


Do you have pics of any of the LC's you have going right now?




OK, here are a few samples of what's cooking.

Mexican strain from Quilla (thanks, CaptainFuture). This has pretty much filled the LC, and if I don't withdraw some of it soon it will form floating pads on the surface.


A well-used GT culture (from a local friend), used to inoculate 7 jars so far. I'm done with this because it's been so prodigious. It has formed substantial mycelial mats on the surface.


Commerical tarragon oyster culture from a syringe, expanding it to a pint jar. It's only been there for a few days. Much of what you see is sediment from the MEA powder.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: sonoramo]
    #27042807 - 11/16/20 09:49 PM (1 year, 2 months ago)

Following this.

I have had beautiful looking, clear LC's with strong myc growth that when put to agar are contaminated. I have been lucky with LC's with over 95% success rate, but this has happened more than once. My SOP now includes a mandatory agar test before using.

I would love a visual "test", but i am not sure its possible and/or accurate. There are a multitude of bacteria and molds and each would elicit a different visual outcome IMO.

On a side note: I notice nutrient LC's seem to have a higher affinity to contamination. When i use a simple 4% karo solution, I usually have no issues.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 795
Loc: Equestria? Mordor? Wester... Flag
Last seen: 1 day, 7 hours
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: maxmush]
    #27044391 - 11/17/20 08:43 PM (1 year, 2 months ago)

Mostly posting to subscribe, but for some reason my LC broths always come out of the PC as a really nice golden color. Munton's LME like most everyone uses :shrug:


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineLemgrub
Hypnerotomachia poliphili
 User Gallery

Registered: 04/03/18
Posts: 452
Last seen: 3 hours, 27 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: MLPismyOPSEC]
    #27044514 - 11/17/20 10:00 PM (1 year, 2 months ago)

These are both inner tissue LCs. Left one was made 11/14 the right was 11/13. I think I can tell which will be no good at this point. Also think I'll tone down the sugar content next time. They're 4% honey.



--------------------




Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblemushhead
I'm a teapot User Gallery


Registered: 08/22/14
Posts: 1,690
Loc: Dimension J-889
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Lemgrub]
    #27044547 - 11/17/20 10:30 PM (1 year, 2 months ago)


Right is B+ left is APE.
LME heat dissolved into distilled water with a 45min PC cycle.
Some sediment but no other microbes save mycelia in here.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,317
Loc: Year Zero
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
    #27046036 - 11/18/20 08:02 PM (1 year, 2 months ago)

Following, will post some pics in a bit


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineBrownBear
Warrior-Traveler
 User Gallery


Registered: 06/05/20
Posts: 1,523
Last seen: 15 hours, 33 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: D3_Myc]
    #27046219 - 11/18/20 09:19 PM (1 year, 2 months ago)

How would you rate these lc's?



Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineenteogenio
Stranger
Registered: 10/31/20
Posts: 1
Last seen: 11 months, 16 days
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear]
    #27046265 - 11/18/20 09:40 PM (1 year, 2 months ago)

What do you think:



*MS Srynge: GT
*Water/Honey (500ml/4ml PC:30min/15psi)
*Date: 19/10/20


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ


Registered: 06/06/20
Posts: 2,495
Loc: संसार
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: enteogenio]
    #27049841 - 11/21/20 05:47 AM (1 year, 1 month ago)



0.4% LME, PC'd for ~20 mins at 15 psi, noc'd with a pan cyan agar wedge.



0.4% LME, PC'd for ~40 mins at 15 psi, noc'd with a pan cyan needle poke biopsy from a pan cyan agar plate.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,702
Last seen: 7 days, 3 hours
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall]
    #27050583 - 11/21/20 04:59 PM (1 year, 1 month ago)

Sexy

:wellhello:


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ


Registered: 06/06/20
Posts: 2,495
Loc: संसार
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: verum subsequentis]
    #27051448 - 11/22/20 04:04 AM (1 year, 1 month ago)

Forgive me, but I need to check, is that a sarcastic sexy? Or is the LC looking ok? I gather it's near impossible to tell whether an LC is clean just by looking at it?

I'm going to yolo one of the smaller batches today.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,317
Loc: Year Zero
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall]
    #27051693 - 11/22/20 10:24 AM (1 year, 1 month ago)

Img dump of the cultures I have going atm. One at rest and one swirled



The tat spun for a few days before I let it settle. The dark spots are the individual tissue pieces


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ


Registered: 06/06/20
Posts: 2,495
Loc: संसार
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: D3_Myc]
    #27051725 - 11/22/20 10:36 AM (1 year, 1 month ago)

Are these clean/tested LCs?


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,317
Loc: Year Zero
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall]
    #27051742 - 11/22/20 10:49 AM (1 year, 1 month ago)

No, they are “I don’t even know what I’m looking at cultures and could use some interjections”


Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Next >  [ show all ]

Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains


Similar ThreadsPosterViewsRepliesLast post
* Visual diff between contam and Myc. for newbie? Pachanguero 1,968 4 06/18/05 08:34 AM
by drugsaregood
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 8,800 44 05/26/05 03:36 PM
by Aeolus1369
* Whats are some of more visual cube strains?
( 1 2 3 all )
Gr0wer 7,402 57 07/15/04 02:55 PM
by Ice House Shaman
* orange stuff in lc+annoying noob questions reax 107 9 01/06/22 02:44 PM
by Nef
* temperature drops as a cue for pinning TheChiaPetFarmer 2,388 13 08/19/04 11:02 AM
by deafpanda
* Is my LC done for? deva1 1,408 6 04/28/06 11:35 PM
by deva1
* Getting the Most LC into WBS Gatorade 945 5 04/21/06 06:48 PM
by skeletor
* LC question mikeownow 1,195 17 03/05/06 01:15 PM
by hyphae

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta, Tormato
4,292 topic views. 44 members, 233 guests and 42 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2022 Mind Media. Some rights reserved.

Generated in 0.044 seconds spending 0.014 seconds on 19 queries.