Home | Community | Message Board

Kratom Eye
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Next >  [ show all ]
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth * 3
    #27007467 - 10/28/20 02:35 AM (1 year, 2 months ago)

As the title suggests, this experiment aims at producing conclusive results about the topic.
So let's see if it's possible to notice the difference between a clean and a dirty broth.
I used two different recipes for these 5 broths that were sterilized in ~qt jars.


Three of them are made from 0.05% ELME and two are made from diluted rye grain water that had been mixed with 19 parts water.
1 ME broth was inoculated with a tiny opaque/grayish bacterial colony, 1 ME broth was inoculated with a yellow bacterial colony ~1mm in diameter and 1 ME broth was inoculated with a tiny piece of unsporulated mold myc that was still growing on the outer edge of a green sporulating colony. 1 of the GW broths was inoculated with a small wedge containing both cube myc and the opaque bacillus colony, and in the other GW jar was dropped a tiny agar wedge that had both cube and mold myc growing on it.


This PE germination plate contributed with the yellow bacteria and the mold and RW plate blessed us with the one opaque colony.




The broths



I swirled the LC's for 10 seconds each and plan on doing so once a day until test is completed.
Jars are incubating at 25C.
There will be daily updates for this experiment since I already noticed obvious activity in multiple jars.


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 1,245
Loc: Hyperspace Chicken Coop
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27007489 - 10/28/20 03:00 AM (1 year, 2 months ago)

Thanks for doing this.

:threadmonitor:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Baba Yaga] * 1
    #27007624 - 10/28/20 06:38 AM (1 year, 2 months ago)

And it seems that bacteria will fuck up a liquid broth in a matter of hours :shrug:

I placed a spore solution vial inbetween the light and the LC jar as to be able to gauge the anticipated turbidity.
But I guess I should have takes pics every 30min or so lol these photos were taken 8h efter inoculation and that's all folks for this experiment :rofl:





Soooo... If your LC hasn't gone turbid in the last few hours I'd say you can be fairly sure there's not bacterial activity in there. :bongload:


--------------------
Water Tub


Edited by Mateja (10/28/20 06:39 AM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,794
Loc: Milky way
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja] * 2
    #27007627 - 10/28/20 06:42 AM (1 year, 2 months ago)

Some bacteria will cause turbidity some wont. Some bacteria form biofilms because of their secretions. Those make a broth way more turbid than say most species of lactobacillus would and some lacto make no turbidity


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

BTC: 1G7roCpYmNp4VqnKySQeBkaFTc7JMzKLpL
ETH: 0x2091cE981af8129986C50AC0818e1aCA76076768
DOGE: DQNyGtUNHwYUrGECag1VRmMcf6QVeTY4V1


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: bodhisatta]
    #27007640 - 10/28/20 06:58 AM (1 year, 2 months ago)

I will continue this experiment for the sake of documenting different types of bacteria and mold growth in LC's to learn to decide easier upon future visual inspections. I can understand how some microbes due to secreting make the broth even more turbid. But are you saying that there are types of bacteria that consist of a perfectly transparent cell? And even when those cells are jammed in the broth in astronomical numbers should there really still be no visible turbidity?


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisibledfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27007813 - 10/28/20 10:01 AM (1 year, 2 months ago)

Good stuff. Now who has a spectrophotometer?

Would be interesting to see what happens when you add bacteria, either via agar like you did or a drop of your new bacteria broth, into a clean colonized or colonizing LC.

Inoculation size might matter in some situations- I'm guessing your additions were orders of magnitudes larger than the start of a typical contam. More of an issue in the case where myc might otherwise be able to fight it.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: dfwerydfhg]
    #27008660 - 10/28/20 05:22 PM (1 year, 2 months ago)

Quote:

dfwerydfhg said:
Good stuff. Now who has a spectrophotometer?



You have two of them on your face, kind of :crazy2:

Quote:


Would be interesting to see what happens when you add bacteria, either via agar like you did or a drop of your new bacteria broth, into a clean colonized or colonizing LC.



Yes I will be doing that as well, adding mold and bacterial colonies to clean LC's at various stages to see how growth is affected.


Quote:


Inoculation size might matter in some situations- I'm guessing your additions were orders of magnitudes larger than the start of a typical contam. More of an issue in the case where myc might otherwise be able to fight it.



Yes these bacterial colonies were gigantic in terms of numbers even tho they were tiny specks. Next round i will just dip the tip of the needle or gently swipe a bacterial colony on agar and then just gently touch the surface of the broth with the tip of the needle. Now that I think of it I actually know a couple of ways to dilute bacterial colonies so I end up with just a handful of cells, I might do that as well if dipping the tip of the needle proves too crude as well.


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineDanger-Dave 1
Stranger

Registered: 04/03/20
Posts: 9
Last seen: 10 months, 27 days
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27008677 - 10/28/20 05:34 PM (1 year, 2 months ago)

Yeah! Thank you for doing this experiment! this needed to be done!


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,702
Last seen: 11 days, 20 hours
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Danger-Dave 1]
    #27008740 - 10/28/20 06:10 PM (1 year, 2 months ago)

:havesomescience:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,881
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: verum subsequentis]
    #27008754 - 10/28/20 06:21 PM (1 year, 2 months ago)

Oh shit dude, was it my penis that gave you that nasty plate? I think I can even see some AIDS there too. Cool experiment tho.
:threadmonitor:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27008813 - 10/28/20 06:55 PM (1 year, 2 months ago)

I've seen much worse :lol:
and if I didn't have faith in your work I'd probably put the swabs to more than 2 plates. :shrug:
I'm pretty sure I found a handful of cube germ spots in this one plate that I transferred, I'll know in a day or two :typing:


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,881
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27008834 - 10/28/20 07:11 PM (1 year, 2 months ago)

I always assume a swab is dirty though. These fruits were all flopping big dicks bending over with the caps touching the surface, many of them with the gills facing upwards, so some nastiness is to be expected.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27008941 - 10/28/20 08:11 PM (1 year, 2 months ago)

At no point did I ever consider that the swabs you sent me were clean lol I just have faith that you're not sending me a catastrophe worth shit.
I also have faith in my ability to get dirty spores to germinate successfully on a grow medium :bongload:


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27015688 - 11/01/20 02:38 PM (1 year, 2 months ago)

This time I inoculated two new broths with 10ml of mold each and then I added
one drop of bacterial solution to one of them and I gently dipped the tip on the bacterial needle into the other broth.
The jar that got a drop of bacterial solution immediately colonized the broth within hours and completely stopped the mold in its tracks. The broth that was dabbed with the bacterial needle tip offered the mold a chance to gain some momentum for probably 24h or so but the day after inoculation I already noticed obvious turbidity. That's also why one of the broths has a larger mold colony than the other, as visible in the pics. When it comes to the bacterial activity in these broths I'd say both reached 'stationary phase' within 36h, the onset of turbidity was gradual but extremely fast and hasn't increased since. So far I'm observing maximum cell mass reached within a couple of days in 200-300ml broth.


Here is the 'drop' and the 'needle tip' inoculated on 31st




And just for reference, here are two homogenized mold LC's with crystal clear broths.
One is MEA 0.05% the other is boiled rye broth diluted with 19 parts water.
Lately I've been placing this Pan Cyan vial inbetween the broths and the light source as a means to detect turbidity. I don't have a spectrophotometer so the text on the spore solution vial is my tool for measurements. On the other hand I don't really have much need for precise measurements or estimations using devices since I only need to detect any turbidity whatsoever or the complete lack thereof.


Mold in MEA and rye water



The Pan Cyan vial is behind the bacterial LC's as well but the light diffusion inside the solution makes is virtually impossible to see, light scatters all over the place trying to shine through a solution crammed with ("invisible") cells.


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,881
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27015704 - 11/01/20 02:50 PM (1 year, 2 months ago)

That has been my experience, the few times I got bacteria in an LC it would go to shit fast from just a little needle poke. It got turbid very quickly and the cube myc reacted to the bacteria in a pretty obvious way, it would slow down its growth too. I for one am dying to see this sneaky bacteria that won't cause turbidity in the liquid that all people talk about, the one that only shows it's ugly face on agar, or even an LC that only has 'a little of bacteria' after being colonizing for more than one week.  :ninjalike:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27015767 - 11/01/20 03:31 PM (1 year, 2 months ago)

Practically every type of bacteria that's in our home/substrates divide every 20-30min on average in good conditions. They look wimpy as hell on agar but in liquids they talk a whole lot of smack, really fast as well and they back that shit up. Reinforcements are always right around the corner and you're already fucked
:itseveryone:


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/03/09
Posts: 6,973
Loc: illinois
Last seen: 1 hour, 14 minutes
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja] * 1
    #27015804 - 11/01/20 03:54 PM (1 year, 2 months ago)

:takingnotes:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,881
Loc: Flag
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: tripdawg420]
    #27015874 - 11/01/20 04:42 PM (1 year, 2 months ago)

I would like to see you try inoculating a healthy 100% cube LC with bacteria, that should be interesting too. Now that you're all in with this I'm sure you won't mind. :grin:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMateja
Antivaxxer
 User Gallery

Registered: 07/14/16
Posts: 7,149
Loc: Tinfoil land
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27015952 - 11/01/20 05:53 PM (1 year, 2 months ago)

Quote:

Josex said:
inoculating a healthy 100% cube LC with bacteria



No sweat man, I was gonna play the villain anyway :bongload:

Was thinking of squirting moderate/excessive amounts of bacterial solution into a fully or mostly colonized myc LC and then immediately use that to inoculate cakes and grain. Been thinking of doing this for a long time cause I'm curious to see how bacterial gangs go about handling their business on the turfs. I've never (I'm assuming no one has) seen bacteria in action on grain with visible visibility from the eyes only. I'd just pour a 50/50 solution myc/bac on top of rye to see if myc colony will be able to implement their policy and ideology or if they'd all be corrupted resulting in bad guys taking over and ruining the society. And then I'd do the same to cakes. This test will probably have varying results tho depending on the water activity of the grain and cake turf it encounters.


Okej kourrwa I have all the passwords and the keys are activate, I just have to mine some more Mycelium to put inside the warhead together with Bacillus so it's will be weaponize properly kourrwa!
:strangelove:


--------------------
Water Tub


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,702
Last seen: 11 days, 20 hours
Trusted Cultivator
Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Mateja]
    #27016056 - 11/01/20 07:00 PM (1 year, 2 months ago)

I think it'd be interesting to test an LC on agar and verify it is clean. Then inoculate something. Then spit in it, put it on a stir plate or swirl it and noc something else. Then noc a new jar every day and track the progress.

But aint nobody got time for that.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Visual diff between contam and Myc. for newbie? Pachanguero 1,970 4 06/18/05 08:34 AM
by drugsaregood
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 8,803 44 05/26/05 03:36 PM
by Aeolus1369
* Whats are some of more visual cube strains?
( 1 2 3 all )
Gr0wer 7,409 57 07/15/04 02:55 PM
by Ice House Shaman
* orange stuff in lc+annoying noob questions reax 107 9 01/06/22 02:44 PM
by Nef
* temperature drops as a cue for pinning TheChiaPetFarmer 2,397 13 08/19/04 11:02 AM
by deafpanda
* Is my LC done for? deva1 1,408 6 04/28/06 11:35 PM
by deva1
* Getting the Most LC into WBS Gatorade 945 5 04/21/06 06:48 PM
by skeletor
* LC question mikeownow 1,195 17 03/05/06 01:15 PM
by hyphae

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta, Tormato
4,309 topic views. 27 members, 196 guests and 47 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2022 Mind Media. Some rights reserved.

Generated in 0.04 seconds spending 0.013 seconds on 18 queries.