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FredsGotSlacks
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T3 Transfers, Need Advice
#27006763 - 10/27/20 05:16 PM (3 years, 3 months ago) |
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Hey Everyone I’d just like to thank everyone for all the posts and knowledge. Been reading for a while and finally within the last month or so, decided to take the plunge. So thanks to all the contributors and TC’s for all you put into the website.
Need some advice on a few T3 transfers. Wondering if they need more transfers or I’m transferring junk or I didn’t completely F things up and looking decent. Background: Variety is B+, Transferred 7 days ago on the 21st, LME Agar - 10g Agar and 10g LME to 500ml water, Pictures are front and backs. Originally from a MSS Thanks
1. Front

1. Back

2. Front

2. Back

3. Front

3. Back
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FredsGotSlacks
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Any help would be greatly appreciated
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majorlove
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Dont know exactly how b+ myc is supposed to look like but that dos not look ready to grain in my eyes. Does not look strong. If its mss you may want to isolate? It may contains many variations of strain aswell.
But im a TOTAL newbie and still learning, just working with cambodia myc on agar.
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FredsGotSlacks
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Re: T3 Transfers, Need Advice [Re: majorlove]
#27009928 - 10/29/20 09:15 AM (3 years, 2 months ago) |
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Thanks for the reply MajorLove, appreciate it. Yeah I was thinking a transfer or 2 more also, but it just isn’t a white as I thought it should be. I just can’t tell if it’s something else. It took 8 days to get that big, and from what I’ve read, if it was contam, it could grow to that size over night. So something tells me it’s not quite contam, but it’s not as white and fluffy as I’ve seen in a lot of pictures (if it’s Tomentose). Even went over Tmethyl’s “How it should and shouldn’t look” and it doesn’t quite look Ike it should, but doesn’t exactly look off. Just has me stumped.
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IdiotCircusBoy
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I'm a noob but according to, Mushroom_Cultivator
"Rhizomorphic mycelia run faster, form more primordia and in the final analysis yield more mushrooms than cottony mycelia"
In other words you want to isolate for the stringy stuff and not the fluffy stuff. Ultimate goal is to isolate for fast growing myc that will produce fruits that are potent.
-------------------- Just call me Idiot "People hasten to judge in order not to be judged themselves."
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Neowynd8

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All mold i think
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John_1098
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Re: T3 Transfers, Need Advice [Re: Neowynd8]
#27010163 - 10/29/20 11:18 AM (3 years, 2 months ago) |
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Looks like mycelium to me but hard to tell by picture, I'd put #2 onto grain to see what happens. I would take more transfers from 1 and 3.
Worst case scenario, its some kind of mold and you lose a couple jars. The middle of #1 front looks funny but again I cant tell even on my 55" 4k tv I use as a monitor. After you throw some slices into a couple grain jars (if you decide to) leave a bit in the plate so you can give it a good up close sniff test. I bet youll be able to tell right away if its good or not by the smell.
-------------------- Don't listen to anything I say, I'm likely 10 grams deep at this very moment
  
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John_1098
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Re: T3 Transfers, Need Advice [Re: John_1098]
#27010164 - 10/29/20 11:19 AM (3 years, 2 months ago) |
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any chance you have pics of the germination plates or earlier ones? I think youre good honestly. Ive had cultures look exactly like that and perform amazingly on grain.
Unless I cut back on the LME my cube cultures always come out tomentose. I use a couple grams less than the 10g / 500ml ratio. I use around 6 or 7 for 400ml water and get rhizo growth on almost every cube plate. Also I pour them pretty thin.
From what I understand its outdated into that rhizo stringy growth means the culture will be a better fruiter. Even consistant healthy growth is what you want to look for.
just my 2cents anyone correct me if Im wrong. Im no expert by any means but Ive got a lots of expirence growing cube myc in petri dishes
Edited by John_1098 (10/29/20 11:44 AM)
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FredsGotSlacks
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Re: T3 Transfers, Need Advice [Re: John_1098]
#27010177 - 10/29/20 11:30 AM (3 years, 2 months ago) |
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Thanks for chiming in IdiotCirusBoy, Neowynd8 and John_1098.
John, will have to wait for later tonight to get pics of early germination and previous transfers. The germ plates will be grown over but my T2 pasty’s should still be good. They aren’t on plates which will make for some crappy photos but maybe just bite the bullet and open them up for (hopefully) better pics.
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John_1098
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I edited my post with a better answer
-------------------- Don't listen to anything I say, I'm likely 10 grams deep at this very moment
  
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El Chupacabra
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Re: T3 Transfers, Need Advice [Re: John_1098]
#27010258 - 10/29/20 12:12 PM (3 years, 2 months ago) |
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10g LME with 500ml is a little nute rich. Mycelium tends to look more cottony and less rhizo on nutritious media. I'd try a lighter recipe for T4 plates and see if you get some more organized rhizo growth
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verum subsequentis
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2 percent is not too rich. I get rhizo on 2 percent all the time.
Quote:
IdiotCircusBoy said: I'm a noob but according to, Mushroom_Cultivator
"Rhizomorphic mycelia run faster, form more primordia and in the final analysis yield more mushrooms than cottony mycelia"
In other words you want to isolate for the stringy stuff and not the fluffy stuff. Ultimate goal is to isolate for fast growing myc that will produce fruits that are potent.
Ancient info. Disregard.
OP. The pics are a little hard to tell exactly but i don't see any problems. I'd advise one more transfer just to be safe. If everything remains fine, I'd then go to grain. Alternatively, You could try and get a few pics that really show nice detail and I'll chime back in. Sometimes having front light and taking an up close pic from an angle can get you a nice clear pic.
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pureshrooming
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Just looks a little weak to me but I've used plates that looked similar and they did fine. I would suggest the same as above, cut your nutes down and see how the transfers react. Try out 7g/500ml
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FredsGotSlacks
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Thanks so much everyone for chiming in. Really appreciate the all the help. The reason for the high nute is because it was left over LME Germination Agar to get things going and I was waiting for 500 dishes to arrive. Hopefully these pics are a bit better, if not I’ll try for some other ones.
Pic 1 - Revised

Pic 2 - Revised

Pic 3 - Revised

Also, here’s my T2 Pasty’s in case anyone is interested. These were created on Oct 15th.

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Neowynd8

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At least they look uniform lol...grain is cheap SEND IT!!! Hahaha
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IdiotCircusBoy
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Re: T3 Transfers, Need Advice [Re: John_1098]
#27010896 - 10/29/20 05:56 PM (3 years, 2 months ago) |
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Quote:
John_1098 said:
From what I understand its outdated into that rhizo stringy growth means the culture will be a better fruiter. Even consistant healthy growth is what you want to look for.
You're totally right John. I did a deep dive into Rhizomorphic vs Tomentose and that info is outdated. Now you just have to learn how to spell "consistent"
-------------------- Just call me Idiot "People hasten to judge in order not to be judged themselves."
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verum subsequentis
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Quote:
Ancient info. Disregard.
That's why i said this.
op. I think you're not growing cube myc. I've been wrong before but i have never seen cube myc look that fucked.
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D3_Myc
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Looks suspect as all hell to me. Very funky... I'd streak some new plates if it was me.
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FredsGotSlacks
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Re: T3 Transfers, Need Advice [Re: D3_Myc]
#27011490 - 10/30/20 04:43 AM (3 years, 2 months ago) |
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Thanks verum and D3monic for the confirmation Going to make some new plates with less nute and swab some soon. Going to keep at least 1 plate for research and to help practice my agar technique Thanks for all your help everyone
Edited by FredsGotSlacks (10/30/20 09:00 AM)
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plut0
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Thats mycelium alright....but I don't think it's cube mycelium
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John_1098
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Re: T3 Transfers, Need Advice [Re: plut0] 1
#27012350 - 10/30/20 03:39 PM (3 years, 2 months ago) |
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Yea I couldnt tell in the first pics but Id bet whatever is growing there smells like embalming fluid thats been pissed in by a dehydrated crackhead
-------------------- Don't listen to anything I say, I'm likely 10 grams deep at this very moment
  
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verum subsequentis
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Re: T3 Transfers, Need Advice [Re: John_1098] 1
#27012918 - 10/30/20 09:23 PM (3 years, 2 months ago) |
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Very descriptive. Excuse me while i hurl.
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plut0
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I bet any of those agar transfers would fruit a FIRE trich tub
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GrinchGrower
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Re: T3 Transfers, Need Advice [Re: plut0]
#27013128 - 10/31/20 12:15 AM (3 years, 2 months ago) |
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Quote:
plut0 said: I bet any of those agar transfers would fruit a FIRE trich tub
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