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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27003641 - 10/25/20 08:51 PM (3 years, 3 months ago)

Makes sense, thank you. Would you suggest that on your 2nd-3rd transfer, you might want to let it grow all the way to each edge of the plate and fully colonized the plate, maybe even wait for pins to occur.. To make sure no contams pop up?


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: NecroMyce] * 1
    #27003646 - 10/25/20 08:54 PM (3 years, 3 months ago)

Hell no. Contams are generally harder to see on an over colonized plate. Once you have a clean culture (don't think about number of transfer, just transfer until clean) let it grow out 3/4 of the plate and then put to grain. I like to put one plate to four masters but you can stretch it further for sure.


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Offlinefightingcherries
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27003753 - 10/25/20 10:12 PM (3 years, 3 months ago)

Have you noticed much variation from cloning a mushroom from spawn that was from a clone?  My master froze in a shitty fridge, so I had to clone when my tub fruited to try to get my culture back.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: fightingcherries]
    #27003794 - 10/25/20 10:40 PM (3 years, 3 months ago)

I've only cloned a clone once and it went wonderfully. Never cloned a clone of a clone though. I wouldn't advise against trying it.

You gotta watch out for shitty fridges. Specially ones with freezers in them.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27003800 - 10/25/20 10:42 PM (3 years, 3 months ago)

Also, girlfriends that accidentally leave the door open and cause everything to freeze.

:baseballfuck:


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OfflineGrinchGrower
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27003878 - 10/25/20 11:55 PM (3 years, 3 months ago)

Quote:

verum subsequentis said:
Also, girlfriends that accidentally leave the door open and cause everything to freeze.

:baseballfuck:




:tryingnottodie:

Seriously though, thanks for all the good info here for noob agar work verum! Got a couple spore to LME plates of PFC and AC just starting to show some growth that I am excited to work with soon.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: GrinchGrower]
    #27003883 - 10/26/20 12:05 AM (3 years, 3 months ago)

My pleasure.
:chesire:


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27003959 - 10/26/20 02:42 AM (3 years, 3 months ago)

I just made 60 plates, did about 5 transfers of each strain. Tossed on that went too long and had a bunch of contams. So I assume as transfers go on, I’ll have a bunch of masters and I also dropped some fresh fruit to plates. I’m excited to see what’ll come in the future. So say, they grow out to 3/4 the plate, we can put them in the fridge at normal fridge temps which I believe is 34-40 F. And this will stall growth for a while, can you put unused agar plates that are already prepped for storage in the fridge as well? What do you think shelf live is?


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OfflineBrotherDekatessera
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Re: Agar transfer, avoiding isolation [Re: Big_Dub]
    #27004383 - 10/26/20 10:50 AM (3 years, 3 months ago)

Quote:

Big_Dub said:
you always want to make tiny transfers. the point of transfering is to get clean healthy mycelium. less is more

look for nice spots, and take those. dont get caught up worrying up sectors. just grow some mushrooms





tbh this is usually the case, just stop worrying and grow more mushrooms.

this latest experiment was the first one where I went simple and easy with transfers, and honestly got the fastest healthiest myc I have seen in a long long time.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: BrotherDekatessera]
    #27004561 - 10/26/20 12:30 PM (3 years, 3 months ago)

Fresh plates don't need cold stored. They last a long as time sitting on a shelf. The slower they dry out the longer they last.


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27004741 - 10/26/20 02:22 PM (3 years, 3 months ago)

Quote:

verum subsequentis said:
Fresh plates don't need cold stored. They last a long as time sitting on a shelf. The slower they dry out the longer they last.



I’m talking about fresh plates that have not been transferred to, I made a shit load so I don’t have to do it again for a while, just curious what best storage options are.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27004832 - 10/26/20 03:01 PM (3 years, 3 months ago)

i know


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27004924 - 10/26/20 04:01 PM (3 years, 3 months ago)

Okay cool. Thank you so much bro! I made a bunch extra, wrapped them
Up and put them in plastic bags until further needed!


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27004932 - 10/26/20 04:03 PM (3 years, 3 months ago)

Just no that temperature fluctuations will lead to condensation in the plates and that is not good. If you don't have relatively stable temps I'd suggest putting them in an insulated box to slow down temp swings.


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27004940 - 10/26/20 04:06 PM (3 years, 3 months ago)

Mine on the shelf will fluctuate 2-6 degrees maybe. But In the fridge it’s stable, that’s why I was asking about cold storage, it’s stable, but not sure if it’ll kill the shelf life.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: NecroMyce]
    #27005071 - 10/26/20 05:21 PM (3 years, 3 months ago)

fridges are less stable than you might think. It depends on the quality of the fridge and a few other factors. You're room will be fine.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27005072 - 10/26/20 05:22 PM (3 years, 3 months ago)

I pour 50 to 60 plates at a time, wrap them or put them in ziploc bags and keep them at room temperature. Use as needed.


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InvisibleStipe-n CapMDiscord
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27005086 - 10/26/20 05:32 PM (3 years, 3 months ago)

Quote:

verum subsequentis said:
fridges are less stable than you might think. It depends on the quality of the fridge and a few other factors. You're room will be fine.




Definitely, I've lost a lot of decent cultures to iced up plates.


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Offlineverum subsequentis
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Re: Agar transfer, avoiding isolation [Re: Stipe-n Cap]
    #27005095 - 10/26/20 05:38 PM (3 years, 3 months ago)

Freezerless mini fridges are tits.


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InvisibleNecroMyce
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Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27005107 - 10/26/20 05:46 PM (3 years, 3 months ago)

Quote:

verum subsequentis said:
Freezerless mini fridges are tits.



That’s what I have :smile: but once you have your clean master culture, you store in the fridge to stall growth for long term storage?


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