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BrotherDekatessera
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My pictures etc.
#27004356 - 10/26/20 10:36 AM (3 years, 3 months ago) |
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these MSS dubtub experiments finally paid off. these three Costa Rica MSS colonized and started pinning in about 7 days
(read anywhere from 6 to 8 because my time awareness is retarded)


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BrotherDekatessera
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These Costa Rica bags from agar slurry LI are just about fully done, 4 days in.
This was the first "isolation" ..
went from MSS to agar, transferred the first couple of spots to germinate from a few dishes, two of those grew out looking much like what most people would call a "monoculture" .. never seen such clean, uniform growth on a first transfer so I went to grain, i.e. these bags. as you can see, the Costa Rica First round, second selection is a boss.

not doing bad at all for four days, but also not pushing as hard as the 1:2, the first round, first selection :
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BrotherDekatessera
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this PF Classic was a disaster.
MSS to 20 agar dishes. 14 contammed' which is about 13 more than my average rate.
5 did nothing.
transferred the only 4 healthy-ish cultures, 10 dishes, 5 contammed.
agar slurried the remaining 2 genetics (keeping a slice of course) and 1 of the bags contammed.
here is whats left.

not bad for four days in, but looking like a retard so far next to the Costa Rica. maybe just an unlucky syringe. I dont know, since Ive never run PF classic before.
is it usually this much of a pain in the ass??
Edited by BrotherDekatessera (10/26/20 10:51 AM)
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Pastywhyte
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Registered: 09/15/12
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It’s easier when you take control of things and stop shooting spores in sketchy bags of grain.
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BrotherDekatessera
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Quote:
Pastywhyte said: It’s easier when you take control of things and stop shooting spores in sketchy bags of grain.
I know that. My main "inoculation" method is agar slurry LI. the dubtub mss were just fuck around experiments because after diluting syringes for plate work, I wanted to play around with the remaining syringes. I have almost unlimited space / tubs to play with and hadnt gone mss to grain in a long time. Same reason I have a wine glass packed with sub and grain colonizing, just fuck around.
As for the PF, it didnt do well anywhere, at all, plate-bag-jar, etc. The MSS random squirt of costa rica is far outperforming the "isolated" PF in every meaningful metric
Edited by BrotherDekatessera (10/27/20 07:19 AM)
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Pastywhyte
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Registered: 09/15/12
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What are the meaningful metrics at the point you’re at?
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BrotherDekatessera
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Quote:
Pastywhyte said: What are the meaningful metrics at the point you’re at?
to me, meaningful metrics at this point in fucking with new spores would be
1. speed of growth on agar 2. speed of colonization in bags/jars 3. speed of colonization of substrate. 4. contam rate (i understand that in a controlled grow much contam is due to human error but there is also a balance of the myc's natural defense ability) 5.structure of the cultures.
1. no comparison 2. no comparison 3. no comparison 4. no comparison, the costa rica didnt have a single failure at any stage 5. the first grab I did from germinating spores on dishes, the costa rica was already close to perfect. the PF just absolutely flailed about the dish leaving uneven hell everywhere. (though in fairness I just checked some super slow dishes and the PF myc there seems a bit better.. not saying much though)
of course, the costa rica might pull a plot twist and not produce much overall, and the PF might blow totes out in the long run, I guess we will see, but for now I can only go by the observable factors at this stage.
I was especially impressed how the one random selection of costa rica went aerial within a day of being spawned and just overran the surface of the sub so fast I couldnt tell if it was webmold for a about a day, created a beautiful little casing of aerial mycelium, and it pinned super fast and its pushing hard the last 24 hours. Ive never seen a tote do that, the rest of the sub is not fully colonized, but it just went crazy on the surface from day one, at this point the rest of the sub is close, but it was curious to see it attack the surface so vigorously and jump start, Im not sure if its genetic or that tote just won the "atmospheric conditions" lottery. I snatched a sample off the surface and will be cloning from that tote for sure, which is what makes random dubtub fuckery interesting
what would you consider meaningful metrics to clock? I'd bet a few totes you have a lot more experience than me. On and off a few times I have a total of about 3 years in monotubs and dubtubs.
Edited by BrotherDekatessera (10/27/20 10:22 AM)
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Pastywhyte
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Registered: 09/15/12
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Well a shitty isolate or clone will consistently perform shitty. Usually has nothing to do with the name on the spore sample the genetics were obtained from. If your cultures are not performing get rid of them and find ones that do.
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BrotherDekatessera
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