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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
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Community Experiments and Projects Thread π¬ 8
#27003791 - 10/25/20 10:40 PM (3 years, 3 months ago) |
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Welcome To the Community Experiments and Projects Thread
This is a place where we can share experiments and projects we are currently working on and update in a singular post.
All are welcome to participate and the rules are simple.
- Title your post
- State your intentions:
be it an experimental procedure, design or testing out new recipes and concepts.
- Formulate a hypothesis:
A prediction of what will be found at the outcome of your project work.
- Share your experiments to test your hypothesis
- Continue to update your original post:
Utilize the edit button to update your OP with the results and conclusions of your experiments or project work.
Side conversations are acceptable and are to be expected but for the sake of keeping the thread clean, organized and searchable keep your experiments and project work contained within your original post.
- Have fun:
Keep in mind not everyone is a scientist or have backgrounds in mycology. Realize that some experiments may be flawed or lack proper controls. You can offer suggestions to the OP's to advance their work but try not to flame, harass or belittle them in regard to their attempted experiments.
- Experiments can be open to the public:
Other members of the community may opt in to help test your hypothesis with their own experiments and share their data with you.
I will post the links to your experiments below with your thread title so they are easily accessed by others.
I look forward to seeing everyone's work and sharing our progress with one another.
Community ProjectsThe Potential Negative Effects of Erythrosine (Red#3) on Mycelium. A Comparison of Growth on Assorted Dyed Agar MediumsInfluence of agar stiffness and nutrition on mcyelium
Edited by D3_Myc (11/02/20 09:24 PM)
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Community Experiments and Projects Thread [Re: D3_Myc] 5
#27003803 - 10/25/20 10:44 PM (3 years, 3 months ago) |
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Edited by D3_Myc (11/06/20 11:01 PM)
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ModularMind
M.P.F.



Registered: 02/09/10
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Re: Community Experiments and Projects Thread [Re: D3_Myc] 1
#27003854 - 10/25/20 11:28 PM (3 years, 3 months ago) |
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Doctor Mario
πππππ



Registered: 08/07/20
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Re: Community Experiments and Projects Thread [Re: ModularMind] 1
#27005487 - 10/26/20 10:02 PM (3 years, 3 months ago) |
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Hell yes, thank you for doing this.
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SingularFusion


Registered: 10/31/18
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Re: Community Experiments and Projects Thread [Re: Doctor Mario]
#27006097 - 10/27/20 11:14 AM (3 years, 3 months ago) |
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pureshrooming
feels like a stranger


Registered: 05/28/18
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Re: Community Experiments and Projects Thread [Re: SingularFusion]
#27006171 - 10/27/20 11:42 AM (3 years, 3 months ago) |
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Well this explains a lot...I randomly have lackluster plates for seemingly no reason and the only food coloring I've ever used is red. I'm gonna be getting some new colors today and see what happens on my end.
I would be interested to find out if the red#3 affects further transfers off of it or would the mycelium bounce back if transferred to say orange or black.
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dfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
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Re: Community Experiments and Projects Thread [Re: pureshrooming]
#27006775 - 10/27/20 05:24 PM (3 years, 3 months ago) |
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following
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mushboy
modboy



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Re: Community Experiments and Projects Thread [Re: dfwerydfhg] 1
#27006903 - 10/27/20 06:35 PM (3 years, 3 months ago) |
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Red never gave me issues


I only use a few drops tho.
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JHOVA
Post whore


Registered: 02/17/17
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Re: Community Experiments and Projects Thread [Re: mushboy]
#27006911 - 10/27/20 06:38 PM (3 years, 3 months ago) |
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i bet if you took a transfer from the red plate to a blue plate it would outpace the donor plate in a few days.
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mushboy
modboy



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Posts: 32,274
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Re: Community Experiments and Projects Thread [Re: JHOVA]
#27006921 - 10/27/20 06:42 PM (3 years, 3 months ago) |
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Lile, I've transfer between agar colors all the time. Without any thought or order or noticable effect
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Community Experiments and Projects Thread [Re: mushboy]
#27006981 - 10/27/20 07:10 PM (3 years, 3 months ago) |
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What brand you using? Maybe different dye used
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mushboy
modboy



Registered: 04/24/05
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Re: Community Experiments and Projects Thread [Re: D3_Myc]
#27006992 - 10/27/20 07:14 PM (3 years, 3 months ago) |
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McCormick. Basic shit in the grocers isle.
Isnt food coloring sugar? I use 2drops for 1000ml. Maybe making the color too deep is adding too much sugar?
Shit: google says most food coloring is made from oils like petroleum??
Quote:
Much red food coloring, known as carmine or cochineal, is made from a white insect that exudes a bright red color when it is crushed.
Edited by mushboy (10/27/20 07:19 PM)
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Community Experiments and Projects Thread [Re: mushboy]
#27007149 - 10/27/20 08:35 PM (3 years, 3 months ago) |
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I use red dye all the time. For a couple years now. I haven't noticed any issues but I only used one drop per 500ml. Plates will even turn back to normal agar color when it's fully colonized.
As for the brand, I can't remember what I have.Cheapest option online I'm sure.
Edited by Nobler Hino (10/27/20 08:40 PM)
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Community Experiments and Projects Thread [Re: pureshrooming]
#27007906 - 10/28/20 09:01 AM (3 years, 2 months ago) |
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Quote:
pureshrooming said: Well this explains a lot...I randomly have lackluster plates for seemingly no reason and the only food coloring I've ever used is red. I'm gonna be getting some new colors today and see what happens on my end.
I would be interested to find out if the red#3 affects further transfers off of it or would the mycelium bounce back if transferred to say orange or black.
I can try that after they have grown out a bit. I would like to test it against a few other brands as well. Perhaps the wilton gel has a higher concentration of erythrosin than say the non gel coloring. Looking at Mc Cormicks red dye looks like they use both Red 40 and Red #3 . They also have a natural dye that uses beet juice for the red coloring. Just depends on the kind you buy. In this case I used 1 drop per 100ml of the wilton neon gel
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Mr.ButterBurger
ope sorry

Registered: 09/02/20
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Re: Community Experiments and Projects Thread [Re: D3_Myc]
#27008721 - 10/28/20 03:58 PM (3 years, 2 months ago) |
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This is a great experiment! I am interested if other dyes show similar antifungal properties.
I just made PDA with some of this colored agar I haven't used before.

It contains Red #40 and so far is doing well. Actually outperforming my MEA formula...
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Community Experiments and Projects Thread [Re: mushboy]
#27008782 - 10/28/20 04:35 PM (3 years, 2 months ago) |
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Quote:
mushboy said: McCormick. Basic shit in the grocers isle.
Isnt food coloring sugar? I use 2drops for 1000ml. Maybe making the color too deep is adding too much sugar?
Shit: google says most food coloring is made from oils like petroleum??
Quote:
Much red food coloring, known as carmine or cochineal, is made from a white insect that exudes a bright red color when it is crushed.

Itβs amazing some of the stuff the food and drug industry gets away with. I remember watching a βhow itβs madeβ type deal on how cod fish sperm is used as a binder in lipstick. They spent a while interviewing this poor dude whoβs sole industry role is hovering over a conveyor belt extracting fish sperm all day... makes one appreciate a few of their life choices. Unless they are into that sort of thing lol
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pureshrooming
feels like a stranger


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Re: Community Experiments and Projects Thread [Re: D3_Myc]
#27009024 - 10/28/20 06:55 PM (3 years, 2 months ago) |
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I just picked up an assortment of colors in gel and regular so I'll be whipping some plates up tonight. Honestly tho that black charcoal seems like the best option to not have to worry about any random ingredients.
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Doctor Mario
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Re: Community Experiments and Projects Thread [Re: pureshrooming]
#27011074 - 10/29/20 08:05 PM (3 years, 2 months ago) |
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I knew I wasn't trippin. I also used q drop of the red food coloring per 100mL water. Its like the shit just wouldn't expand and when it finally did, it grew underneath the agar and moved very slowly probably because it had to fight to get through the agar.
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MycFunk3D
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Re: Community Experiments and Projects Thread [Re: Doctor Mario]
#27012577 - 10/30/20 06:12 PM (3 years, 2 months ago) |
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Great idea love to see what comes of this thread
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
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Re: Community Experiments and Projects Thread [Re: MycFunk3D]
#27014901 - 10/31/20 11:47 PM (3 years, 2 months ago) |
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I've updated the coloring experiment and have poured some additional plates to add to it. Anyone else got any fun stuff they are working on they would like to share?
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