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InvisibleD3_Myc
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Community Experiments and Projects Thread πŸ”¬ * 8
    #27003791 - 10/25/20 10:40 PM (3 years, 3 months ago)

Welcome To the Community Experiments and Projects Thread


This is a place where we can share experiments and projects we are currently working on and update in a singular post.

All are welcome to participate and the rules are simple.


  • Title your post

  • State your intentions: be it an experimental procedure, design or testing out new recipes and concepts.

  • Formulate a hypothesis: A prediction of what will be found at the outcome of your project work.

  • Share your experiments to test your hypothesis

  • Continue to update your original post: Utilize the edit button to update your OP with the results and conclusions of your experiments or project work.

    Side conversations are acceptable and are to be expected but for the sake of keeping the thread clean, organized and searchable keep your experiments and project work contained within your original post.

  • Have fun: Keep in mind not everyone is a scientist or have backgrounds in mycology. Realize that some experiments may be flawed or lack proper controls. You can offer suggestions to the OP's to advance their work but try not to flame, harass or belittle them in regard to their attempted experiments.

  • Experiments can be open to the public: Other members of the community may opt in to help test your hypothesis with their own experiments and share their data with you.


I will post the links to your experiments below with your thread title so they are easily accessed by others.

I look forward to seeing everyone's work and sharing our progress with one another.

Community Projects


The Potential Negative Effects of Erythrosine (Red#3) on Mycelium. A Comparison of Growth on Assorted Dyed Agar Mediums

Influence of agar stiffness and nutrition on mcyelium


Edited by D3_Myc (11/02/20 09:24 PM)


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InvisibleD3_Myc
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Re: Community Experiments and Projects Thread [Re: D3_Myc] * 5
    #27003803 - 10/25/20 10:44 PM (3 years, 3 months ago)

The Potential Negative Effects of Erythrosine (Red#3) on Mycelium. A Comparison of Growth on Assorted Dyed Agar Mediums

Background: Anecdotal reports suggest that mycelia grown on agar containing red#3 a artificial  dye called Erythrosine may cause irregular, sporadic or stunted growth of mycelia. Erythrosine is an artificial red (cherry-pink) food coloring made from coal tar. It is an organic compound containing iodine and sodium. Limited research suggests that Erythrosine may have natural fungicidal properties.

The intent of this experiment will be to test out growth patterns of mycelium on agar dyed with various colors of food coloring to determine if any have benefits over the others or perhaps hinder growth.

Hypothesis: The agar plates containing erythrosin (red#3) or in this case Wilton neon gel magenta food coloring will have a negative impact on mycelium growth.

Experiment:

All agar recipes consist of 20g agar agar, 12.5g Light malt extract and 1000ml of distilled water. Dyes selected are Wilton Neon Gel "Magenta", "Teal" and organic charcoal powder. ( I realize a control plate with no coloring would have been beneficial to the experiment but time did not allot having some poured in time. Hopes is that the charcoal powder (black plates) are on a color media that shouldn't be providing any additional nutrients in the form of corn sugars or other ingredients that are found in the gel food coloring.

Sample #1



Transfers where taken on 10-23 from equally healthy looking growth using a custom agar punch tool keeping transferred samples uniform in size and shape.





Sample #2



Again transfers taken on 10-23



All test subjects are stored in a manor that they receive similar light and rotated every two days to ensure equal lighting and heating conditions are obtained.



Update 10-26


Sample #1



Sample #2



Update 10-27-20


Fairly easy to tell there’s there’s a difference in growth rate between the red plates and other dishes.

Sample 1



Sample 2




Update 10-29-20

I think it's fairly evident that there's stunted maybe even halted growth on the plates containing Erythrosine (Red #3) Compared to the other plates.

Sample 1



Sample 2




I don't see any noticeable differences between the blue or black plates in growth does anyone else really?

For my next samples I will pour the same nute levels but no dye, purple (which contains both red #40 and red #3 ) orange, blue and black and do a comparison.

Update 10-31 Happy Halloween!! πŸ‘» πŸŽƒ


This is my final update with this batch of samples. I’ll let you draw your own conclusions based on the photographic evidence provided



For my next round I’m expanding to include

  • Purple

  • orange

  • No coloring


Stay tuned

Unofficial final final pic.. lol




Edited by D3_Myc (11/06/20 11:01 PM)


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InvisibleModularMind
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Re: Community Experiments and Projects Thread [Re: D3_Myc] * 1
    #27003854 - 10/25/20 11:28 PM (3 years, 3 months ago)

:havesomescience:

:superscience:


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InvisibleDoctor Mario
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Re: Community Experiments and Projects Thread [Re: ModularMind] * 1
    #27005487 - 10/26/20 10:02 PM (3 years, 3 months ago)

Hell yes, thank you for doing this.


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OfflineSingularFusion
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Re: Community Experiments and Projects Thread [Re: Doctor Mario]
    #27006097 - 10/27/20 11:14 AM (3 years, 3 months ago)

:takingnotes:


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Offlinepureshrooming
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Re: Community Experiments and Projects Thread [Re: SingularFusion]
    #27006171 - 10/27/20 11:42 AM (3 years, 3 months ago)

Well this explains a lot...I randomly have lackluster plates for seemingly no reason and the only food coloring I've ever used is red. I'm gonna be getting some new colors today and see what happens on my end.

I would be interested to find out if the red#3 affects further transfers off of it or would the mycelium bounce back if transferred to say orange or black.


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Invisibledfwerydfhg
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Re: Community Experiments and Projects Thread [Re: pureshrooming]
    #27006775 - 10/27/20 05:24 PM (3 years, 3 months ago)

following


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InvisiblemushboyMDiscord
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Re: Community Experiments and Projects Thread [Re: dfwerydfhg] * 1
    #27006903 - 10/27/20 06:35 PM (3 years, 3 months ago)

Red never gave me issues:shrug:




I only use a few drops tho.


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InvisibleJHOVA
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Re: Community Experiments and Projects Thread [Re: mushboy]
    #27006911 - 10/27/20 06:38 PM (3 years, 3 months ago)

i bet if you took a transfer from the red plate to a blue plate it would outpace the donor plate in a few days.


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InvisiblemushboyMDiscord
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Re: Community Experiments and Projects Thread [Re: JHOVA]
    #27006921 - 10/27/20 06:42 PM (3 years, 3 months ago)

Lile, I've transfer between agar colors all the time. Without any thought or order or noticable effect:shrug:


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InvisibleD3_Myc
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Re: Community Experiments and Projects Thread [Re: mushboy]
    #27006981 - 10/27/20 07:10 PM (3 years, 3 months ago)

What brand you using? Maybe different dye used


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InvisiblemushboyMDiscord
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Re: Community Experiments and Projects Thread [Re: D3_Myc]
    #27006992 - 10/27/20 07:14 PM (3 years, 3 months ago)

McCormick. Basic shit in the grocers isle.

Isnt food coloring sugar? I use 2drops for 1000ml. Maybe making the color too deep is adding too much sugar?:strokebeard2:

Shit: google says most food coloring is made from oils like petroleum??

Quote:


Much red food coloring, known as carmine or cochineal, is made from a white insect that exudes a bright red color when it is crushed.





:justdontknow:


Edited by mushboy (10/27/20 07:19 PM)


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InvisibleNobler Hino
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Re: Community Experiments and Projects Thread [Re: mushboy]
    #27007149 - 10/27/20 08:35 PM (3 years, 3 months ago)

I use red dye all the time. For a couple years now. I haven't noticed any issues but I only used one drop per 500ml. Plates will even turn back to normal agar color when it's fully colonized.

As for the brand, I can't remember what I have.Cheapest option online I'm sure.


Edited by Nobler Hino (10/27/20 08:40 PM)


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InvisibleD3_Myc
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Re: Community Experiments and Projects Thread [Re: pureshrooming]
    #27007906 - 10/28/20 09:01 AM (3 years, 2 months ago)

Quote:

pureshrooming said:
Well this explains a lot...I randomly have lackluster plates for seemingly no reason and the only food coloring I've ever used is red. I'm gonna be getting some new colors today and see what happens on my end.

I would be interested to find out if the red#3 affects further transfers off of it or would the mycelium bounce back if transferred to say orange or black.




I can try that after they have grown out a bit. I would like to test it against a few other brands as well. Perhaps the wilton gel has a higher concentration of erythrosin than say the non gel coloring. Looking at Mc Cormicks red dye looks like they use both Red 40 and Red #3 . They also have a natural dye that uses beet juice for the red coloring. Just depends on the kind you buy. In this case I used 1 drop per 100ml of the wilton neon gel


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OfflineMr.ButterBurger
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Re: Community Experiments and Projects Thread [Re: D3_Myc]
    #27008721 - 10/28/20 03:58 PM (3 years, 2 months ago)

This is a great experiment! I am interested if other dyes show similar antifungal properties.

I just made PDA with some of this colored agar I haven't used before.


It contains Red #40 and so far is doing well. Actually outperforming my MEA formula...


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InvisibleD3_Myc
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Re: Community Experiments and Projects Thread [Re: mushboy]
    #27008782 - 10/28/20 04:35 PM (3 years, 2 months ago)

Quote:

mushboy said:
McCormick. Basic shit in the grocers isle.

Isnt food coloring sugar? I use 2drops for 1000ml. Maybe making the color too deep is adding too much sugar?:strokebeard2:

Shit: google says most food coloring is made from oils like petroleum??

Quote:


Much red food coloring, known as carmine or cochineal, is made from a white insect that exudes a bright red color when it is crushed.





:justdontknow:




It’s amazing some of the stuff the food and drug industry gets away with. I remember watching a β€œhow it’s made” type deal on how cod fish sperm is used as a binder in lipstick. They spent a while interviewing this poor dude who’s sole industry role is hovering over a conveyor belt extracting fish sperm all day... makes one appreciate a few of their life choices. Unless they are into that sort of thing lol


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Offlinepureshrooming
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Re: Community Experiments and Projects Thread [Re: D3_Myc]
    #27009024 - 10/28/20 06:55 PM (3 years, 2 months ago)

I just picked up an assortment of colors in gel and regular so I'll be whipping some plates up tonight. Honestly tho that black charcoal seems like the best option to not have to worry about any random ingredients.


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InvisibleDoctor Mario
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Re: Community Experiments and Projects Thread [Re: pureshrooming]
    #27011074 - 10/29/20 08:05 PM (3 years, 2 months ago)

I knew I wasn't trippin. I also used q drop of the red food coloring per 100mL water. Its like the shit just wouldn't expand and when it finally did, it grew underneath the agar and moved very slowly probably because it had to fight to get through the agar.


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OfflineMycFunk3D
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Re: Community Experiments and Projects Thread [Re: Doctor Mario]
    #27012577 - 10/30/20 06:12 PM (3 years, 2 months ago)

Great idea love to see what comes of this thread


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InvisibleD3_Myc
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Re: Community Experiments and Projects Thread [Re: MycFunk3D]
    #27014901 - 10/31/20 11:47 PM (3 years, 2 months ago)

I've updated the coloring experiment and have poured some additional plates to add to it. Anyone else got any fun stuff they are working on they would like to share?


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