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Offlinethe man
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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27028570 - 11/08/20 01:01 PM (3 years, 2 months ago)

hmm ya might not be the best idea. if glycerin or sugars or alcohol would affect growth negatively or too much iodine of course kills myc ie use in surgery and brewing to kill/clean.  does someone reccomend adding iodine?  i suspect just one spray would be enough for supplementation if that was the idea?

cheers


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: the man]
    #27028676 - 11/08/20 01:59 PM (3 years, 2 months ago)

I haven't seen anyone recommend it personally, I'm just trying it mostly as a contamination barrier. I've used it in a cloning process (alongside bleach) before and it worked, so I'm seeing if it is a viable option. Similar to bleach, Iodine/iodide dilute doesn't kill Mushroom mycelium. Maybe it will act as a stressor like the aspirin does in the above article and make them produce more Ganoderic Acid.


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Offlinethe man
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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27029373 - 11/08/20 11:08 PM (3 years, 2 months ago)

mushrooms create wonderful natural barriers some say the best stuff for protection. :wink: happy myc doesnt need extra stuff :smile: of course clean area doesnt hurt but obv causing more issues then nything. plus u dont want to od on iodine and u dont want to eat too much bleachy salts


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: the man]
    #27029520 - 11/09/20 03:56 AM (3 years, 2 months ago)

I drink J.Crows in my water fairly often, and more than what I put in the water sprayer. My thyroid is working just fine, I just had it checked at my last doctors visit. Thanks for your concern :smile: I'm not sure what you are getting at with the reference to bleach, could you elaborate your concerns better? (are you suggesting the amount that was used it cloning and rinsed off is going to make it to the fruits?)

Did you know Lugol's Solution is commonly used to purify (Drinking) water?

:themoreyouknow:


Edited by teknix (11/09/20 05:23 AM)


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: the man]
    #27029531 - 11/09/20 04:11 AM (3 years, 2 months ago)

Also, thanks for bringing it up; here is a link to some risks and benefits that can be associated to Lugol's solution. It should go without saying, but don't drink bleach, straight iodine or peroxide! :lol: J. Crows (2%), which I am referring, is a Lugol's formulation, which isn't the same as Tincture Iodine. (Tincture Iodine is labeled as "External Use Only")

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693970/


Table 1
Examples of usage of Lugol’s solution and other iodine preparations

Graves’ disease
Disinfectant
Sterilisation
Foot ulcers
Dental procedures
Histopathology
SchillerΒ΄s test
Water purification
Radiation emergency
Iodine deficiency/ cretinism
Protection from goiter

And this article also elaborates on the confusion of using measurements in drops versus ml and the dangers associated with it.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693970/

You can also use bleach for water purification, but I think it would taste nasty, and I kinda like the way iodine tastes:

Purifying by adding liquid chlorine bleach

Treat water by adding liquid household bleach, such as Clorox or Purex.

Household bleach is typically between 5.25 percent and 8.25 percent chlorine. Read the label.

Avoid using bleaches that contain perfumes, dyes and other additives. Be sure to read the label.

Cloudy water should be filtered before adding bleach.

Place the water in a clean container. Add the amount of bleach according to the table below.

Mix thoroughly and let stand for at least 60 minutes before drinking.

(Mixing table in link below)

https://www.doh.wa.gov/Emergencies/BePreparedBeSafe/SevereWeatherandNaturalDisasters/WaterPurification


Edited by teknix (11/09/20 05:51 AM)


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Invisiblenosf3r4tu


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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27031331 - 11/10/20 09:55 AM (3 years, 2 months ago)

Hey. I hope your grow is doing well.
I just found some Ganoderma Lucidum in two places today but some where dried and moldy. Still I did find a peace of an antler to be free of mold but it's dry.
Could you share a link or some info about how I should go about cloning that one?


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: nosf3r4tu]
    #27031495 - 11/10/20 11:47 AM (3 years, 2 months ago)

It's going ok, just a bit slow in comparison to the established strains I generally work with.

You want to get the soft white tissue to work with (from inside the antler), or if there isn't any soft tissue just get the whitest tissue.

If you find the method in the OP doesn't work and you are getting contams from the sample you can also add 2 drops of lugols iodine to 250 ml of water and then move it from the bleach dunk (5 min) to the paper towel Then rinse it, and then move it into the iodine dunk (1 min) then to the paper towel and then to the water to rinse it and then dry it on the paper towel and put it on the dish.

You could probably use hydrogen peroxide as well, but I'm not sure on what would be an effective dilute and time. You could probably put it in pure 3% peroxide for a minute but I would probably start with a 50% dilute of 3% peroxide for 1 minute if I was going to try it. (If I was using bleach first, if I wasn't using bleach first and just H2O2 dilute I would do 5 minutes)

Oh, and if you are trying to do it open air like I did, it's kinda tricky, you have to open the dish by just lifting up the lid straight up with just enough room to put the specimen on the agar, holding the lid directly above the dish and put the specimen in the dish with forceps/tweezers fairly quickly and then put the top on the dish right away. ( I personally just open a part of the dish farthest away from me at an angle, but it's harder to see what you are doing that way) The if you want to put another specimen in the same dish just rotate it a bit and do the same thing. It's really only tricky at first though. And make sure you are wearing gloves and a mask at the least. The gloves I was using are dish gloves that go up to my forearm. But I had also scrubbed the air with an air purifier prior to doing it.


Edited by teknix (11/10/20 12:25 PM)


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Invisiblenosf3r4tu


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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27032776 - 11/11/20 04:56 AM (3 years, 2 months ago)

Thx.
I already cloned them and I followed the same procedure as for the previous ones I did. I think they'll do fine. The inside of the antlers weren't completely dry. Just a bit dehydrated.


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Offlinethe man
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Re: Wild Reishi Clone Grow. [Re: nosf3r4tu]
    #27033190 - 11/11/20 10:43 AM (3 years, 2 months ago)

I thought u said u were spraying your fruited bags with iodine solution and previously sprayed them on the regular with bleach solution...


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27035150 - 11/12/20 12:13 PM (3 years, 2 months ago)

Quote:

teknix said:






The blue in the mutant block was from dunking it in bleach when it got contaminated, but I haven't personally ever used bleach in my water sprayer. I never processed those after dunking them in bleach, I just left them to their own accord. That block is completely dry and like 3-4 years old now. I just kept it around because it was weird, hehe.


Edited by teknix (11/12/20 12:19 PM)


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27142449 - 01/11/21 05:31 PM (3 years, 17 days ago)

The clone bags stalled out and it looks like it was due to a hyphomycetes contam, and it is because I failed to obtain a pure culture. Using the bleach method. The resilience to bleach extends to lower fungus, so it didn't kill them either. t.t



On a brighter note, the Lion's Mane is doing pretty well in vitro on the unsupplemented fuel pellets!



I got a decent conk and lots of spores from the one of the ones in the humidity tent.



and antlers from the other:



And all the reishi growing in vitro in bags look pretty sad :frown: I plan on letting the first flush grow in vitro, then I want to try dunking them in water with iodine and instant coffee, lol :smile:



The shiitake tubs are browning:




I also made some bigger reishi block I supplemented with instant coffee this time:



And that's about it.


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Invisibleteknix
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Re: Wild Reishi Clone Grow. [Re: teknix] * 1
    #27252155 - 03/14/21 07:17 AM (2 years, 10 months ago)

My best conk yet! (2 antlers from 2 different bags are making this 1 conk, lol.) This is from a couple bags I supplemented with Instant Coffee and water with J.Crows dilute:



:laugh:

Massive Shiitake caps:





Ended up with 2 quarts dried reishi and 1 quart dried Lion's mane from the invitro Reishi and Lion's mane:




Edited by teknix (03/14/21 10:31 AM)


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InvisibleGenericHero
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Re: Wild Reishi Clone Grow. [Re: teknix]
    #27254856 - 03/15/21 10:27 PM (2 years, 10 months ago)

Good reading. Thanks y'all for making my evening less boring.


--------------------
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