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OfflineSphongler
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Extracting psilocybin crystals from moldy mushrooms
    #27002751 - 10/25/20 01:19 PM (3 years, 3 months ago)

Hello,

This may be a very dumb question but thought I would ask and find out!
I just started my first batch of psilocybin cubensis in a mono tub about a month ago. Something came up and I had leave them to be attended by a friend who does not have knowledge of mushroom cultivation. The first growth went fine and he harvested them. This was a test run so I got a relatively small yield.

I told them how to properly harvest them with a sterile technique but I guess it went over their head and they harvest the following flushes reusing gloves, not sterilizing tools and the such. Not their fault, just an awkward situation needing to do this over distance. They face timed me, but with the blue red grow lights I was using and bad image quality I could not see the contamination. The following flushes were moldy, with a small amount on mold in the top of the cap.

As the title of my post suggests, I was wondering if these specimens would be suitable to extract crystals from? This is something I wanted to try. Since it is a specific compound being extracted from the fungal matter would it be contaminated from the mold? I would lean towards just throwing them out but I thought it wouldn’t hurt to ask the pros for my own personal knowledge.

Thanks!


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OfflineDnDRnD
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Sphongler]
    #27002778 - 10/25/20 01:40 PM (3 years, 3 months ago)

Dont have much experience doing extracts besides making tea but I do know most purer psilocin/psilocybin extracts tend to break down relatively quickly from oxygen exposure and light exposure.

I more actually wanted to chime in to mention that harvesting mushrooms is never sterile, especially because they are never fruited in a sterile environment to begin with, if your substrate contaminates after the first or second flush it is almost always an indicator of bad spawn that was contaminated in the very beginning

When healthy mycelium has 100% colonized a substrate you could literally spit or sneeze on it without it becoming contaminated (I do NOT recommend spitting on your substrate it's just an example of how resistant to contam 100% percent colonized substrate is)

Hope that makes harvesting easier in the future since you dont have to worry about sterilizing more tools or anything!  :thumbup:


--------------------
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Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

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https://www.shroomery.org/forums/showflat.php/Number/26009662

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OfflineInnerWisdom
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: DnDRnD]
    #27002791 - 10/25/20 01:47 PM (3 years, 3 months ago)

Sounds like a hassle and possibly a waste of time.
Just grow more.


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OfflineInnerWisdom
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Sphongler]
    #27002795 - 10/25/20 01:48 PM (3 years, 3 months ago)

First time I hear of sterile harvesting too. The shrooms aren't growing in a sterile environment so why bother? Totally unnecessary


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OfflineBig_Dub
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: InnerWisdom]
    #27002973 - 10/25/20 03:17 PM (3 years, 3 months ago)

no such thing as sterile harvesting technques, bc you are not growing in sterile conditions

if you are asking this type of question, you dont understand enough about chemistry to perform a rxn safely


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split_by_nine said:
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do the right thing


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OfflineSphongler
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: DnDRnD]
    #27003001 - 10/25/20 03:44 PM (3 years, 3 months ago)

Thanks! That’s really good information for a noobie. Ya I thought it should be more resilient once they started fruiting as well, my friend is going to be relieved to hear it wasn't their fault. They definitely felt worse about it than I did haha.

I used rye berries to grow the mycelium. I was busy with work so I had left them for months and there was no contamination I could see. My substrate on the other hand was coconut coir and I wasn’t fully confident I was able to sterilize it as fully. Do you think it was more likely the substrate or the rye berry spawn?


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OfflineBig_Dub
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Sphongler]
    #27003004 - 10/25/20 03:46 PM (3 years, 3 months ago)

you dont sterilize the coir/substrate.

you sterilize your grain that you add the mycelium too.


--------------------
split_by_nine said:
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do the right thing


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OfflineSphongler
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: InnerWisdom]
    #27003017 - 10/25/20 03:56 PM (3 years, 3 months ago)

Hahaha ok l, thanks for putting it bluntly. I have been trying to practice sterility from a-z and I haven’t read anywhere about there being no such thing as sterile harvesting technique. This is great to know as it will save time and I will be able to narrow down where the contamination possibly happened. Thought my first try was going a bit too smoothly hahaha. Do you think the first flush is safe for consumption? Or should I just toss everything and start new?
Thanks again!


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OfflineSphongler
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Big_Dub]
    #27003042 - 10/25/20 04:13 PM (3 years, 3 months ago)

Ok interesting. Obviously I need to take a step back and learn more. I followed another tutorial and a guy put his coconut coir in a pressure cooker to sterilize it.
I didn’t see any sign of contamination from the agar work, cloning (live in Cali so I didn’t buy an lc), lc I made and in my grain spawn jars. Only saw the characteristic white mycelium. Can competing molds hide out for that long without growing? Or maybe my inexperienced eye didn’t detect contamination.
Thanks for the help!


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OfflineSphongler
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Big_Dub]
    #27003046 - 10/25/20 04:15 PM (3 years, 3 months ago)

No I haven’t done any research into this extraction technique yet so your right I don’t know enough. Just a hypothetical question for the future if there was a point in keeping these mushrooms.


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OfflineDnDRnD
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Sphongler]
    #27003078 - 10/25/20 04:33 PM (3 years, 3 months ago)

Coco coir is pasturized not sterilized

Sterilizing coco coir opens it up to contamination although coco coir itself is relatively contam resistant anyways but the "Bucket TEK" is the most used coco coir prep method

How did you make the LC? Bacterial colonies can be harder to see and if it's bad enough can weaken the mycelium enough to open it to contamination from competing molds quicker

If the rye berries were to wet that can make them more prone to bacterial contamination as well

If the mushrooms have mold on them then they arnt safe to eat although mold has a hard time growing on FRESH mushrooms, you should post pics for better judgement though it may be bruising your seeing and not mold on the mushrooms themselves but without pics it's hard for us to tell


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat


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OfflineSphongler
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: DnDRnD]
    #27003302 - 10/25/20 06:17 PM (3 years, 3 months ago)

Oh really! Thats great info! I should have followed the bucket tek on here but went with the instapot out of inexperience. As well this was probably the area I did the least amount of research so that may be the issue. Thanks again for the help! Much appreciated

Made 4 jars of liquid culture. Used 300ml of water and 3tsp of honey for each one.
I pressure cooked them in the insta pot for 20 mins. Using an instapot may be an issue, I couldn’t find a clear answer if it is suitable for this kind of work. I listened to a handful of people that swear by it including one professional grower. I left the pressure cooker until it reached
atmospheric pressure. I then sterilized the jars with alcohol %71 and put them into a sterilized glove box. Went for a more overkill method with sterilization just to try to be on the safe side. Everything around my work area I sprayed with alcohol, left the room to sit, no air flow, vacuumed the floor prior, had a shower, put on clean clothes, used latex gloves and a mask.
Pressure cooked all my instruments that are wrapped in tin foil for an hour (this is an area that might have an issue, couldn’t find a clear answer if you should wrap instruments or they need to touch the steam directly). Sterilized wrapped instruments with alcohol and added them to the glove box. Agar dish was wiped down with alcohol and added to glove box. Cutting out a wedge of agar, I put them into each liquid culture jar then I numbered them 1-4 for reference.

I used the rye berry recipe from fresh cap, posted the link.
https://blog.freshcapmushrooms.com/learn/how-to-make-perfect-grain-spawn-for-growing-mushrooms-at-home/

I also numbered the spawn jars 1-4 and inoculated them accorded to the LC numbers. I used the same sterilization techniques as I did before. Used a different syringe for each jar. Once the mycelium grew I didn’t see any contamination. This was a small batch just to test out my first try and so save materials if something went off. As it did haha. I added the small jars of inoculated rye berries to half a brick of coconut coir. When I had to leave, the mono tub looked fine with white mycelium growing on top. I may probably should have put a casing layer on top but I heard it may be useless so decided against it. The image with the cap has some grey looking mold towards the center, may be hard to see in the pic. As well the first flush had much different caps, more the type I am used to seeing with more of a golden color and wrinkled cap.











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OfflineDnDRnD
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: Sphongler]
    #27003341 - 10/25/20 06:36 PM (3 years, 3 months ago)

Quote:

Sphongler said:
Oh really! Thats great info! I should have followed the bucket tek on here but went with the instapot out of inexperience. As well this was probably the area I did the least amount of research so that may be the issue. Thanks again for the help! Much appreciated

Made 4 jars of liquid culture. Used 300ml of water and 3tsp of honey for each one.
I pressure cooked them in the insta pot for 20 mins. Using an instapot may be an issue, I couldn’t find a clear answer if it is suitable for this kind of work. I listened to a handful of people that swear by it including one professional grower. I left the pressure cooker until it reached
atmospheric pressure. I then sanitized the jars with alcohol %71 and put them into a sanitized glove box. Went for a more overkill method with sterilization just to try to be on the safe side. Everything around my work area I sprayed with alcohol, left the room to sit, no air flow, vacuumed the floor prior, had a shower, put on clean clothes, used latex gloves and a mask.
Pressure cooked all my instruments that are wrapped in tin foil for an hour (this is an area that might have an issue, couldn’t find a clear answer if you should wrap instruments or they need to touch the steam directly). sanitizedwrapped instruments with alcohol and added them to the glove box. Agar dish was wiped down with alcohol and added to glove box. Cutting out a wedge of agar, I put them into each liquid culture jar then I numbered them 1-4 for reference.

I used the rye berry recipe from fresh cap, posted the link.
https://blog.freshcapmushrooms.com/learn/how-to-make-perfect-grain-spawn-for-growing-mushrooms-at-home/

I also numbered the spawn jars 1-4 and inoculated them accorded to the LC numbers. I used the same sterilization techniques as I did before. Used a different syringe for each jar. Once the mycelium grew I didn’t see any contamination. This was a small batch just to test out my first try and so save materials if something went off. As it did haha. I added the small jars of inoculated rye berries to half a brick of coconut coir. When I had to leave, the mono tub looked fine with white mycelium growing on top. I may probably should have put a casing layer on top but I heard it may be useless so decided against it. The image with the cap has some grey looking mold towards the center, may be hard to see in the pic. As well the first flush had much different caps, more the type I am used to seeing with more of a golden color and wrinkled cap.














- alcohol only sanitizes it doesnt sterilize, not a big issue alot of people make that mistake but it's good to remember the difference between sanitary and sterile

- when sterilizing tools in a PC they're always wrapped in foil or else when you take them out of the PC they're automatically exposed to non sterile air so definitely always wrap them in foil

So since theres black mold in the tub definitely dont eat any mushrooms that have been in contact with the black mold

When in doubt throw it out is what I go by

In that pic with the colonizing rye berries you can see where its avoiding growing over some of the grains, that can be from over/under hydration or it could be because there was bacterial colonies beginning to grow there


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat


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OfflineSphongler
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Re: Extracting psilocybin crystals from moldy mushrooms [Re: DnDRnD]
    #27003430 - 10/25/20 07:14 PM (3 years, 3 months ago)

Ah ok. Ya I got to get that in my head, sanitize/sterilize. Good to know about wrapping equipment too, I have read that items need to be in contact with steam to actually kill contaminants on the tools but it’s counter intuitive due to what you said. Also I thought the rye berries looked dry to me and I have in my notes to cook them a bit longer next time.

Would you say the most likely point of contamination was the rye berries or the coconut coir since I pressure cooked it? Of course this is from the limited information you have from an amateur. I’ll try from LC another time and  work on educating myself further. If an issue happens again, I’ll  start from my agar cultures.

Thank you so much for the help, it’s really appreciated! You’re awesome!


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