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Offlinepsycho_nauticus
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RW and PESA shoeboxes
    #27002475 - 10/25/20 10:01 AM (3 years, 3 months ago)

Input is appreciated, please leave me a reply, comment, or whatever you think. Thank you!

I have two RustyWhyte shoeboxes with a 1:1 to 1:2 spawn sub ratio. I made two separate shoeboxes this time with only one myco quart in each beacause my last attempt I doubled the spawn and only came out with a massive blob fest that I had to get rid of.

My PESA shoebox, since it is a little bit bigger, I used both quarts I had into one shoebox.

All were spawned Friday October 23rd and are pictured 48 hours later as of this morning.

RW was a very slow colonizer for me when I put it A2G. I didn't even knock up my PESA jars until a week after my RW, and it was with an old MS syringe I had from a year ago I was messing around with no less, and it caught up and colonized in one week compared to the RW. So it doesn't surprise me that the RW is moving at a very slow rate since being spawned, which you can see down below compared to the PESA.

I have two shoeboxes of RustyWhyte, but one is only pictured since they look the same anyway.

RustyWhyte - spawned Friday October 23rd - 48 hours later. Seems to be a very slow colonizer again, I just hope it doesn't mess up somehow again or if I did something wrong myself. Compare this with the PESA after it and you'll see what I mean.



PESA - also spawned Friday October 23rd - 48 hours later. Now this is the one I put two quarts in, and it is just ripping through the coir and substrate, and has already overtaken RW. You can also see rhizomorphs also breaking the surface also.



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Invisiblefilthyknees
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27004497 - 10/26/20 11:48 AM (3 years, 3 months ago)

:thumbup: did you case?


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: filthyknees]
    #27005270 - 10/26/20 07:53 PM (3 years, 3 months ago)

I put a top layer on all of them. Is that what you mean?


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Invisiblealaskappalachian
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27005472 - 10/26/20 09:45 PM (3 years, 3 months ago)

RW is reliably slow for me as well.  Worth the wait,  but slow AF.  First tub I ever grew was a 16qt 1:2 and it was like 27 days until I got pins.  Was about to give up.  Took a while to isolate something that finished more quickly and my best culture averages 18 days.  Worth the wait though.  My favorite "regular cube" to consume, hands down. I actually get my best BE with RW @ 1:3 and they definitely like that top layer.  :thumbup:


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Invisiblefilthyknees
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Re: RW and PESA shoeboxes [Re: alaskappalachian]
    #27005827 - 10/27/20 07:38 AM (3 years, 3 months ago)

Yes


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: filthyknees]
    #27005848 - 10/27/20 08:00 AM (3 years, 3 months ago)

Then yes I did. I put a top layer on. I was getting confused because I thought casing meant some other type of mix that goes on.


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InvisiblePastywhyteMDiscord
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27005986 - 10/27/20 10:04 AM (3 years, 3 months ago)

I’ve been consistently getting pins in the RW tubs I’ve been running since starting back up in about 10-14 days after spawning. Longer than that would have me questioning the health of my spawn and the vigor of the culture. Put spores to 10 plates. Make 2 transfers from the best of the best. Pitch the rest. Then make 2 or more transfers from what looks good. If nothing looks really good by the 3rd transfer I’d toss it all and start back with spores.

I find people get hung up on spore plates, like if they make enough transfers something good will emerge. Yes it can take 5 or 6 to narrow the good growth but, if you only see sluggish slow growth from the spores and T1 plates it’s doubtful that something amazing will pop up 2 more transfers down the road. If you’re on T2 and nothing stands out, start over.


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: filthyknees]
    #27006686 - 10/27/20 04:29 PM (3 years, 3 months ago)

So should I leave these lids snapped and latched on until they grow and hit the lid? Or do I need to unsnap the lids beforehand? I've heard many different things that these shoeboxes still have sufficient FAE with the lids closed, etc. so will knotting and pinning still occur with the lids shut? Or do I need to unsnap the lids when the sub reaches 100% colonization?


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Edited by psycho_nauticus (10/27/20 04:36 PM)


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InvisiblePastywhyteMDiscord
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27006690 - 10/27/20 04:35 PM (3 years, 3 months ago)

I’m not sure I can answer that question. Personally I put it into fruiting conditions immediately. But I just run monos, shoeboxes are weird to me. Some people do awesome with em no question, just not something I’m well versed in. Also I’d be more concerned with the headroom, are you planning on having a second shoebox go overtop? RW can get pretty tall if they get enough water.


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: Pastywhyte]
    #27006696 - 10/27/20 04:40 PM (3 years, 3 months ago)

No I don't want to dub tub it, I didn't know they get that tall. I was hoping they would grow to about average height where the lid is... I guess I will have to use a grocery bag or something...


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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27006730 - 10/27/20 04:56 PM (3 years, 3 months ago)

I used to see stuff like this all the time



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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: Pastywhyte]
    #27008825 - 10/28/20 05:08 PM (3 years, 3 months ago)

That's what I'm trying to learn also, what my boxes and substrates are telling me and how to read them. They're almost 100 percent colonized, but I haven't opened any lids yet, so I need to check for beads on the surface, but I don't want to do that and pull the lid off until it's fully done colonizing. They've lightened up a little on the condensation on the sides, but there's still a lot on the lids. The corners of all the tubs are dry, and I'm assuming that's because that's where the FAE is coming in from in the shoeboxes.

I can't tell or see any beads of moisture on the surfaces yet since I haven't popped the lids... But here are some updated pictures, 5 days since spawning. Also, it looks like my all clear shoebox with the PESA is retaining more condensation all over the sides sides compared to my shoeboxes with the grey lids.

What do you guys see or conclude so far? Any advice and input is appreciated please!!!

RW



PESA You can see it's a very rhizomorphic surface through the top!)



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Edited by psycho_nauticus (10/28/20 05:22 PM)


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27013397 - 10/31/20 07:32 AM (3 years, 3 months ago)

Do I need to introduce more FAE to initiate knotting?


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InvisiblePastywhyteMDiscord
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27013427 - 10/31/20 08:26 AM (3 years, 3 months ago)

Maybe. I introduce fruiting conditions the day I spawn.


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: Pastywhyte]
    #27013514 - 10/31/20 09:47 AM (3 years, 3 months ago)

Is it a characteristic of RW to look like random splatter when knotting?


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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27013516 - 10/31/20 09:48 AM (3 years, 3 months ago)

It’s prone to blobbing. Pics would help .


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: Pastywhyte]
    #27015321 - 11/01/20 08:43 AM (3 years, 3 months ago)

Pictures below.

I think PESA is going to be the real winner and star here, it's knotting up phenomenally on the surface everywhere, and I think it is going to have a bad ass pin set if there's that many knots covering the entire surface. I have a feeling it's going to be a canopy shoebox... And this was the shoebox I was worried about that I had compressed the substrate too much...

The damn RW boxes aren't really performing how I thought it would be. Some knotting going on on the sides and a few here and there on the surface. Doesn't seem like I'll be getting a whole lot from these... Very disappointing that there's not too much happening with these with all the work and energy going into it and with my second attempt at these. My first attempt I did too high of a ratio of spawn of 2:1 and all I got was blobs. This time I decided to do the regular ratio of one myco quart in each shoebox, somewhere between 1:1 and 1:2.



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InvisiblePastywhyteMDiscord
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27015350 - 11/01/20 08:58 AM (3 years, 3 months ago)

Man your spawn is still too high IMO. I’m doing like 1:3.5 or 1:4 with my RW right now. Make better use of your spawn and see better yields.


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Offlinepsycho_nauticus
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Re: RW and PESA shoeboxes [Re: Pastywhyte]
    #27015356 - 11/01/20 09:02 AM (3 years, 3 months ago)

Well shit, that sucks... You're the first to tell me that high of a sub ratio... I was just told don't over spawn. It would be pretty hard to get a 1:4 in shoebox to fit nicely without the sub almost being to the top of the shoebox... So do these not look right to you?


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Invisiblemind.at.large
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Re: RW and PESA shoeboxes [Re: psycho_nauticus]
    #27015365 - 11/01/20 09:13 AM (3 years, 3 months ago)

Quote:

psycho_nauticus said:
It would be pretty hard to get a 1:4 in shoebox to fit nicely without the sub almost being to the top of the shoebox...



What about splitting a jar between two shoeboxes? :shrug:


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