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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 24 days
Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27028991 - 11/08/20 05:39 PM (3 years, 2 months ago)

I'm using whole oats.

Tried eats prep and bods prep both a couple times now. Oats were coming out of the PC in one big clump that i'm unable to break up.

Tried to get them completely dry on the outside the last two runs and they're definitely a lot less clumpy/ move more freely, not perfect though.

Going to build a strainer like yours in the coming weeks and use box fans beneath like how lotkid does.


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Agar transfer, avoiding isolation [Re: Mr. Mushie]
    #27029044 - 11/08/20 06:20 PM (3 years, 2 months ago)

Yeah. Just hydrate till they are nice and plump and then dry thoroughly. Lack of proper drying is what gets a lot of noobs with oats. I prefer wheat, rye and wbs but oats can work fine.


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Offlinemimir
Professional Noob
I'm a teapot
Registered: 01/21/19
Posts: 212
Loc: Europe
Last seen: 13 days, 7 hours
Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27177885 - 01/30/21 11:56 AM (2 years, 11 months ago)

Figured this isn't too old to resurrect, but wouldn't a solution possibly be to keep cloning at every grow? If as soon as the mycelium is subcultured on agar it starts to sector and lose genotypes left and right, wouldn't cloning its offspring manage to keep everything together every time? Maybe this could be done once sectoring on agar starts to appear, not necessarily with every single grow.


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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
Last seen: 5 days, 13 hours
Re: Agar transfer, avoiding isolation [Re: mimir]
    #27177897 - 01/30/21 12:03 PM (2 years, 11 months ago)

Yup, but many TC's say that even clones are made up of multiple strains, however i think with a clone you are guaranteed to have a fruiting strain everytime since it came from a mushroom. But from multispore there are so many strains that you'd have to transfer many times in order to get a true isolate. I don't think the average grower is in risk of having that happen to them.


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Offlinemimir
Professional Noob
I'm a teapot
Registered: 01/21/19
Posts: 212
Loc: Europe
Last seen: 13 days, 7 hours
Re: Agar transfer, avoiding isolation [Re: Phony Phone]
    #27177907 - 01/30/21 12:17 PM (2 years, 11 months ago)

After reading Masters and slaves hunting clones I guess this is more of a theoretical discussion at this point, as keeping a slant + master plate will avoid any excessive subculturing.

Quote:

Yup, but many TC's say that even clones are made up of multiple strains



I never implied this wasn't the case, I think you're confusing with my other post you replied to


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Agar transfer, avoiding isolation [Re: mimir]
    #27179497 - 01/31/21 02:19 PM (2 years, 11 months ago)

Cloning again and again will lead to an old and weak culture. Cell division = age. It's why we die eventually (Not that that's all there is to it or that i fully understand life and death). When you start again from spores, you are starting a new baby culture. As it grows across the agar plate/ grain jar/ substrate, it is dividing cells over and over and over.

This is precisely why we slant killer cultures and put them in the fridge. At near freezing temps the culture basically quits growing. By doing this you keep your culture at the age it was when slanted (basically). It's like having a sixteen year old you on tap at any moment.


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Offlinemimir
Professional Noob
I'm a teapot
Registered: 01/21/19
Posts: 212
Loc: Europe
Last seen: 13 days, 7 hours
Re: Agar transfer, avoiding isolation [Re: verum subsequentis]
    #27179794 - 01/31/21 06:28 PM (2 years, 11 months ago)

I am assuming the problem of the culture getting old doesn't happen that easily when isolating on agar. You are still expanding the mycelium but the amount of cell divisions that it takes to get to the cell you are biopsying from the mushroom is much larger than that following even hundreds of transfers. Am I correct in this assumption?


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Agar transfer, avoiding isolation [Re: mimir]
    #27181646 - 02/01/21 07:50 PM (2 years, 11 months ago)

Yes you are.


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