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zakzwijn
Stranger
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Looks like trich, can confirm? 1
#27001373 - 10/24/20 02:41 PM (3 years, 3 months ago) |
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Hi fellow shroomers, what do you think this is? I fear it's trich This is still during colonization CO2 buildup phase.

Edited by zakzwijn (10/24/20 02:46 PM)
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bodhisatta 
Smurf real estate agent


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Re: Looks like trich, can confirm? [Re: zakzwijn] 1
#27001383 - 10/24/20 02:45 PM (3 years, 3 months ago) |
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Its definitely contamination.
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El_Flying_Mohawk
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Re: Looks like trich, can confirm? [Re: bodhisatta]
#27001427 - 10/24/20 03:13 PM (3 years, 3 months ago) |
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Sure looks like trich that's about to go green
-------------------- Forever noob
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: Looks like trich, can confirm? [Re: zakzwijn]
#27001439 - 10/24/20 03:19 PM (3 years, 3 months ago) |
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Im afraid so
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zakzwijn
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Yh I already was afraid and even think I'm seeing some smear of green already but my brain tried to tell me that wasn't the case. I'll isolate the tub, cut out the bad part and see if I can salvage something.
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zakzwijn
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Re: Looks like trich, can confirm? [Re: zakzwijn]
#27002106 - 10/25/20 02:10 AM (3 years, 3 months ago) |
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I am speculating about what could be the cause. I am following this method for making substrate:
it tells me to leave the substrate for 24 hours to get it to field capacity. I didn't do this in the first two monotube I made and these turned out fine. Only when I started to leave the substrate for 24 hours I started seeing problems so it could be I gave any fungi present in the coco peat a head start?
What do you think? I'm thinking of letting it cool just for a bit instead so I don't kill the spawn, then transfer straight to monotub and mix. This is the method I'm seeing in spitball's monotub tek and I think it's better.
Gotta all learn from experience and try things out, I guess.
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Forrester
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Re: Looks like trich, can confirm? [Re: zakzwijn]
#27002177 - 10/25/20 04:01 AM (3 years, 3 months ago) |
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Sorry I don't have 8min to watch that video, but if it's going green that early it's probably your spawn that isn't clean.
What's your process for making spawn?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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zakzwijn
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Re: Looks like trich, can confirm? [Re: Forrester]
#27003939 - 10/26/20 01:45 AM (3 years, 3 months ago) |
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My procedures are good and I succeeded making sterile spawn numerous times. Do you know the 'Lets grow mushrooms' vid from the bearded guy? I am doing it like that. You can find those vids on youtube. I am using a flow hood which I built myself, this one:
https://www.shroomery.org/forums/showflat.php/Number/26723926
I inoculated half of the jars with spore syringes I bought and some with home made agar plates. I suspect the agar cultures I made weren't clean. I didn't make agar cultures from spores but from a piece of colonized fungi. Now I also think I was stupid and only put the lid on and didn't seal the agar plates with micropore tape so I suspect some contams crept in all while the colonized agar was LOOKING fine.
I threw away one jar before which was going slightly green and remember another jar which was looking fine, but now I think it could have been contaminated as well but not in the green stage yet... so that could have been the culprit 
Oh well, per aspera, ad astra.
I have four monotubs in total, two of which were contaminated. I have two other monotubs which seem to be doing fine.
Edited by zakzwijn (10/26/20 01:51 AM)
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