Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals Bridgetown Botanicals   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2  [ show all ]
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Moldy basement SAB work (now with video)
    #27000953 - 10/24/20 08:32 AM (3 years, 3 months ago)

Sucks!

Any tips for making this easier? Here is a pic of my setup:



And looking at this pic closely and critiquing my damn self I just realized there is a giant gaping chasm in the middle of the table that is probably causing a giant air current.....

But aside from that massive flaw that I'm going to rectify right now (which may be the root of all my problems) What precautions would you take when working in a moldy basement? Is it okay to be working right off the table? I clean the surfaces in and around the SAB whenever I work, wear gloves to avoid skin flakeage, iso my gloves a couple times while working, flame the scalpel outside the box, keep my deli containers upside down when not working and sideways while working.

Here are the no pours I'm using:



They have a tiny ass hole on the side covered in 3 layers of micropore.

While I'm here, I have a second question. I have had some unopened containers contam on me as well. Not all, but enough to raise an eyebrow. Is this because of my moldy basement? I pour the agar, PC for 45 - 60 mins, let the PC depressurize on its own. Thinking about adding another layer or 2 of micropore. Recipe is 10g agar, 10g LME, 500ml water.

Thanks all


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Edited by McDominator (10/25/20 10:06 AM)


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Moldy basement SAB work [Re: McDominator]
    #27001045 - 10/24/20 09:43 AM (3 years, 3 months ago)

Is there another area you can work in that isnt so moldy?


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: eatyualive]
    #27001075 - 10/24/20 10:27 AM (3 years, 3 months ago)

I wish I could say yes, but I don't have a lot of space and share that space with 2 monster dogs, a kid, and my wife.


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: McDominator]
    #27002410 - 10/25/20 09:21 AM (3 years, 3 months ago)

It's not the best video, I screwed up a couple times, and I know that plate is fubar. And the damn water on the agar. I don't have high hopes for these plates! I just wanted to give an idea of what my process is to see if you guys think it looks okay or if there are any changes I should make. Thanks!





--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Edited by McDominator (10/25/20 09:23 AM)


Extras: Filter Print Post Top
OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester... Flag
Last seen: 10 days, 10 hours
Re: Moldy basement SAB work [Re: McDominator]
    #27003094 - 10/25/20 04:37 PM (3 years, 3 months ago)

You know you dun goofed on the second transfer @ 3:06. Other than that, i don't notice anything major. I feel you move your cups too quick, that's most likely where contams are coming in. Moving too fast stirs up the air. The moldy basement doesn't have much to do with contams, because the SAB works very well to keep that shit out.


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: MLPismyOPSEC]
    #27003117 - 10/25/20 04:48 PM (3 years, 3 months ago)

Oooh. Moving the cups too quick eh? That's an easy thing to fix. In my mindset of trying to keep things open for less time I'm trying to be quick about things. Guess that didn't translate well to the whole process 😅 Relieved to hear my basement may not be a problem after all.

Yea, and that second plate was being a dick. :grin:

Thanks!


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Extras: Filter Print Post Top
OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester... Flag
Last seen: 10 days, 10 hours
Re: Moldy basement SAB work [Re: McDominator]
    #27003292 - 10/25/20 06:15 PM (3 years, 3 months ago)

Fuck that second plate! :lol: I always think of the front of the SAB as a big curtain. If there's no motion in the air to disturb the curtain, the air in the SAB stays perfectly* still, and everything falls straight down. If there's nothing above your plates, there's nothing to fall onto them. It is good thinking to not have your plates open long, but time isn't an issue. I leave them sit open in the SAB for minutes at a time.


Extras: Filter Print Post Top
InvisibleIdiotCircusBoy
Human Person
Male User Gallery


Registered: 09/22/20
Posts: 294
Loc: Flag
Re: Moldy basement SAB work [Re: MLPismyOPSEC]
    #27003357 - 10/25/20 06:40 PM (3 years, 3 months ago)

Is the background noise a washing machine cycling?  It almost sounds like a HVAC fan kicks on at like 1:33.  Can you run a dehumidifier for a day or so and spray down everything in the basement that looks suspicious with mold and mildew cleaner.


--------------------
Just call me Idiot


"People hasten to judge in order not to be judged themselves."


Extras: Filter Print Post Top
Offlinediegosf
scientific dreamer
Male


Registered: 06/30/20
Posts: 93
Last seen: 2 months, 10 days
Re: Moldy basement SAB work [Re: McDominator] * 1
    #27003433 - 10/25/20 07:15 PM (3 years, 3 months ago)

Hi!
Nice to see your work on video.

I also have the same problem of you, with the little pool of water remaining on the plates, so i used to do what you did, let it turned down for a while and then let it drop on the floor of the SAB.
But i started to notice a lot of contams poking on the edges of the agar, days after i open them in the SAB and did the transfers..

So i figure it out that it should be probably because of the water remaining on the very little space between the lid and the cup, that was bringing contams inside, through that line of water..
I tested when i keep the plates down, dont turning up again, and they remain clean.
My plates are kinda different than your though, there is actually a space where the lid is curved enough to hold contams in there..
Probably when i let the water touch the lid, it gathers the bacteria and spores of mold, and when i close and turn right again, they fall through the sides..


Dont know if its your case, but in the video there is a time when you let some drops of water in the lid, and automatically i thought about what used to happen to me.


--------------------


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Moldy basement SAB work [Re: diegosf]
    #27003725 - 10/25/20 09:40 PM (3 years, 3 months ago)

I would recommend Moving a tad more slowly and put a rack of some sort in there to do your work on. The floor of the sab is where all the nasties land. Flipping those bitches over and setting them on the filthy ass floor with the lids cracked and sliding them around is just begging for it. If you don't want a rack, at least put one dish on top of another while working with it.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 58 minutes
Trusted Cultivator
Re: Moldy basement SAB work [Re: verum subsequentis] * 1
    #27003814 - 10/25/20 10:54 PM (3 years, 3 months ago)

Yeah I’m with verum. Having the semi open lids on the ground sliding around is probably the problem.

I’d bet either keeping them right side up or the way you did them but keeping them on top of another container would help a lot.

When you have them upside down and press on the bottom to slide them back it seems like the pressure from your fingers would push air out and then suck in all kinds of junk stirred up from moving them when you took your fingers off.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Moldy basement SAB work [Re: McDominator] * 1
    #27003840 - 10/25/20 11:15 PM (3 years, 3 months ago)

You can put a wet towel under some cookie cooling racks to trap the falling contaminate spores during your still period. They won’t be wafted airborne by slight hand movements as they would from a solid non-porous surface.


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Moldy basement SAB work [Re: ModularMind] * 2
    #27003853 - 10/25/20 11:27 PM (3 years, 3 months ago)

Jesus. I don't miss sabs at all. Totally forgot about the ol wet towel.


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Moldy basement SAB work [Re: verum subsequentis]
    #27003857 - 10/25/20 11:32 PM (3 years, 3 months ago)

Always feels counterintuitive and non-conducive to sterile procedure but yeah.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 58 minutes
Trusted Cultivator
Re: Moldy basement SAB work [Re: ModularMind]
    #27004044 - 10/26/20 05:54 AM (3 years, 3 months ago)

I just had to move my SAB before reading this, here’s my set up that works fine.



The rack is crucial. I still keep a couple empty plates stacked on it that I put the ones I’m working on up on too.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,274
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Moldy basement SAB work [Re: A.k.a] * 1
    #27004099 - 10/26/20 07:14 AM (3 years, 3 months ago)

Quote:

verum subsequentis said:
Jesus. I don't miss sabs at all. Totally forgot about the ol wet towel.




i always worked on a hard surface:shrug: towels are obnoxious af



Quote:

A.k.a said:






eww redbull:tongue:


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: mushboy]
    #27004116 - 10/26/20 07:29 AM (3 years, 3 months ago)

Awesome, thank you guys. Another simple thing to implement! I have a rack I just found that I never use for anything else.

I have about a 70% contam rate right now, but everything you are saying sounds pretty logical as to why that may be the case! :grin:

And I did think about buying or building a flow hood... that might be something I go for in the future.


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,274
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Moldy basement SAB work [Re: McDominator] * 1
    #27004119 - 10/26/20 07:34 AM (3 years, 3 months ago)

i dont know if any one mentioned it. too lazy to check but i wouldnt recommend using the lid as the floor either.

think the game operation. every time you make contact with the box it vibrates. vibrating box is how shit falls into your work. dont touch the box. the lid is attached to the box. loose the lid.


Extras: Filter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 12 hours
Trusted Cultivator
Re: Moldy basement SAB work [Re: mushboy]
    #27004124 - 10/26/20 07:40 AM (3 years, 3 months ago)

idk about a wet towel, but I use a towel as a way to kinda brace the SAB and I like feeling like there is a good "seal" around the bottom


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Filter Print Post Top
Offlineredbee
prole spore threat
Female

Registered: 03/30/20
Posts: 175
Last seen: 1 year, 7 months
Re: Moldy basement SAB work [Re: diegosf] * 1
    #27004174 - 10/26/20 08:44 AM (3 years, 3 months ago)

Quote:

diegosf said:
Probably when i let the water touch the lid, it gathers the bacteria and spores of mold, and when i close and turn right again, they fall through the sides..




Quoted for truth. I'm using similar containers as you, McDominator—same style, but mine are slightly smaller. I've been struggling with contams, but improving. I first tried using these upside-down like pastyplates, and like you do in your video. But I found that I pulled in contams through the condensation water touching the lid and then falling back onto the agar, like diegosf described.

I've switched to using these more like petri dishes, and my contam rate has gone down. I don't EVER flip them upside-down. I try to avoid lifting them off the surface of my rack when I'm transferring. I'll prep a stack of transfers, and crack the lids on each before I flame my tools, then treat them like petri dishes—I lift the lids, make the transfers, set the lids down gently, then re-seal them all once I've finished with a set of transfers. I find that this method also involves way less movement, and I feel like I'm able to keep from stirring the air too much by just lifting lids as opposed to flipping whole plates.

The only time I'll flip them upside-down is if the surface is absolutely covered in water. I'll empty those out quickly, then flip them back right-side up, and do my transfers that way. I then watch those particular plates like a hawk for any contam, for the next few days.

Anyway, thanks a bunch for posting these videos, this has been hugely helpful to see the collective feedback! Keep us posted about how things go, I'm curious to hear what methods you wind up adopting. :toast:


--------------------
:sporedrop:  redbee's LAGM 2021  :sporedrop:


Extras: Filter Print Post Top
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
Re: Moldy basement SAB work [Re: redbee]
    #27004372 - 10/26/20 10:43 AM (3 years, 3 months ago)

I don’t know if anyone’s mentioned this but I used to work for/run crew on a mold/flooded rehabilation crew for homes and we would go and spray. Bleach and alchohol only harm the mold they will make it look like it went away but it will recover with a vengeance. They sell microban online in gallons as medic lean and you can get the gallon and a pump sprayer and dilute it with water and spray the whole basement and that WILL kill any mold/spores on the all the surfaces. Just the surfaces but it’s better than nothing. Periodically I’ll take the pump scraper and just spray surfaces in my grow room for updated cleaning.

Just a thought. Sterile tek is key though. You can use a sab in dirty room and still be alright but it’s good to keep it as clean as you can to limit the accidental mishap


--------------------


To spend just one moment in eternity


Edited by Celestialexplorer1 (10/26/20 10:44 AM)


Extras: Filter Print Post Top
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
Re: Moldy basement SAB work [Re: redbee]
    #27004374 - 10/26/20 10:44 AM (3 years, 3 months ago)

I don’t know if anyone’s mentioned this but I used to work for/run crew on a mold/flooded rehabilation crew for homes and we would go and spray. Bleach and alchohol only harm the mold they will make it look like it went away but it will recover with a vengeance. They sell microban online in gallons as medic lean and you can get the gallon and a pump sprayer and dilute it with water and spray the whole basement and that WILL kill any mold/spores on the all the surfaces. Just the surfaces but it’s better than nothing. Periodically I’ll take the pump scraper and just spray surfaces in my grow room for updated cleaning.

Just a thought. Sterile tek is key though. You can use a sab in dirty room and still be alright but it’s good to keep it as clean as you can to limit the accidental mishap


--------------------


To spend just one moment in eternity


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: redbee]
    #27019063 - 11/03/20 09:57 AM (3 years, 2 months ago)

Quote:

redbee said:
Quote:

diegosf said:
Probably when i let the water touch the lid, it gathers the bacteria and spores of mold, and when i close and turn right again, they fall through the sides..




Quoted for truth. I'm using similar containers as you, McDominator—same style, but mine are slightly smaller. I've been struggling with contams, but improving. I first tried using these upside-down like pastyplates, and like you do in your video. But I found that I pulled in contams through the condensation water touching the lid and then falling back onto the agar, like diegosf described.

I've switched to using these more like petri dishes, and my contam rate has gone down. I don't EVER flip them upside-down. I try to avoid lifting them off the surface of my rack when I'm transferring. I'll prep a stack of transfers, and crack the lids on each before I flame my tools, then treat them like petri dishes—I lift the lids, make the transfers, set the lids down gently, then re-seal them all once I've finished with a set of transfers. I find that this method also involves way less movement, and I feel like I'm able to keep from stirring the air too much by just lifting lids as opposed to flipping whole plates.

The only time I'll flip them upside-down is if the surface is absolutely covered in water. I'll empty those out quickly, then flip them back right-side up, and do my transfers that way. I then watch those particular plates like a hawk for any contam, for the next few days.

Anyway, thanks a bunch for posting these videos, this has been hugely helpful to see the collective feedback! Keep us posted about how things go, I'm curious to hear what methods you wind up adopting. :toast:




So I made myself a bigger SAB, in preparation for eventual G2G transfers and got myself a rack. Recently did transfers without tipping them upside down. Definitely moved more slowly, but I think my hand went over a dish at one point which is an issue I didn't have with them upside down, need to practice. The other new issue I ran into was where to put the damn lids while working and worrying about getting contams on the lids. Always something. Upside down makes that part much easier, but apparently also contam prone. I made a video of my first time trying this new method and decided not to post it because I have a lot of work to do on the technique that I can already see.

I made 2 plates with a culture syringe (king oyster) and one of them has a few spots of mold while the other is squeaky clean. A syringe drop is much easier than cutting and transferring a wedge and I still botched one :rolleyes: So I made 4 transfers out of that dish and we will have to see how those turn out in time.


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: McDominator]
    #27059988 - 11/27/20 11:00 AM (3 years, 2 months ago)

Small update for all who are interested. I made 6 transfers, kept all dishes upside down on raised racks (like 5 inches high). 6 out of 7 dishes became contaminated. (including the source). So now I've stopped going upside down and we will see what happens! Work in progress!


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Extras: Filter Print Post Top
OfflineProfessor X
School for the Gifted
 User Gallery


Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
Re: Moldy basement SAB work [Re: McDominator] * 1
    #27060103 - 11/27/20 12:23 PM (3 years, 2 months ago)

I do all my transfers in front of a flow hood 6 feet from a rat cage with 95+% success rate. It's all about technique. Slow deliberate movements, careful positioning and lots of 70% iso. SABs work awesome in bad conditions. Used one in a carpet room for years. Practice makes perfect.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: Professor X]
    #27083483 - 12/11/20 06:16 AM (3 years, 1 month ago)

Finally a good update!

I made 3 transfers to the deli cups, all 3 failed on me. I have close to a 100% failure rate with those after doing close to 100 transfers.

A few days later I made 3 transfers to the Ziploc twist tops..... No contams. Cleanest growth I've had yet. 3 for 3 going strong.

More experimenting needs to be done, but so far the deli containers are going in the trash. My guess is it has something to do with how the covers come off and snap on. Too much movement happening because they get stuck. With the twist caps, there's no snapping or popping going on whenever I maneuver the lids.



Finally feeling a bit hopeful here after months!


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Edited by McDominator (12/11/20 06:17 AM)


Extras: Filter Print Post Top
OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 2 months
Re: Moldy basement SAB work [Re: McDominator]
    #27092613 - 12/16/20 05:26 PM (3 years, 1 month ago)

Guys, seriously. Went from about a 90% failure rate to a 90% success rate by switching containers. Just wanted to inform you all of what worked in case anyone comes across this. It's probably some sort of user error that was corrected by changing containers. Maybe I don't maneuver the lids of the deli containers as well as others. But this worked, and I'm so stoked. Never felt confident with agar before, but I feel like I'm finally getting there!


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Moldy basement SAB work [Re: McDominator]
    #27094464 - 12/17/20 03:51 PM (3 years, 1 month ago)

Congrats


Extras: Filter Print Post Top
OfflineTheBoJim
Strangest
 User Gallery

Registered: 07/23/20
Posts: 449
Last seen: 10 months, 8 days
Re: Moldy basement SAB work [Re: McDominator]
    #27094946 - 12/17/20 08:49 PM (3 years, 1 month ago)

Quote:

McDominator said:
Guys, seriously. Went from about a 90% failure rate to a 90% success rate by switching containers. Just wanted to inform you all of what worked in case anyone comes across this. It's probably some sort of user error that was corrected by changing containers. Maybe I don't maneuver the lids of the deli containers as well as others. But this worked, and I'm so stoked. Never felt confident with agar before, but I feel like I'm finally getting there!




good to hear! and you learned some better techniques along the way. why not go for disposable plates?


--------------------


Edited by TheBoJim (12/17/20 08:50 PM)


Extras: Filter Print Post Top
Offlinenektar61S
Into SporePlay
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 18 hours
Re: Moldy basement SAB work (now with video) [Re: McDominator]
    #27194681 - 02/09/21 01:50 AM (2 years, 11 months ago)

What about making a little room inside the basement...using a wood frame and  plastic of some kind.

They did this on Breaking Bad for that mobile army field hospital that Gus had set up in Mexico. Then Walt did the same thing when they were cooking inside the houses that were tented for bug spraying.

Just make one that's much smaller. Enough for just your chair and a small desk, big enough for your SAB and no more.


--------------------
-NEW? Start here.


Extras: Filter Print Post Top
OfflineDigDug
Cultivator


Registered: 05/05/20
Posts: 201
Last seen: 7 months, 6 days
Re: Moldy basement SAB work [Re: nektar61]
    #27194784 - 02/09/21 04:53 AM (2 years, 11 months ago)

Your aseptic technique looks pretty good.  I agree with the others.  Either do your work on a rack, or soak a paper towel in isopropyl and do your work on there.  You'll cut down contams a lot this way.  Nice job.


Extras: Filter Print Post Top
Offlinenektar61S
Into SporePlay
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 18 hours
Re: Moldy basement SAB work (now with video) [Re: McDominator]
    #27195131 - 02/09/21 09:37 AM (2 years, 11 months ago)

Quote:

McDominator said:
Sucks!

Any tips for making this easier? Here is a pic of my setup:








I'd tape up the big crack above your right arm hole. at least on the outside, probably with duct tape. Or clear packing tape.

What are the wires and rods on top of your SAB around the weight?


--------------------
-NEW? Start here.


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Moldy basement SAB work (now with video) [Re: nektar61] * 1
    #27195424 - 02/09/21 12:10 PM (2 years, 11 months ago)

Lightning


Extras: Filter Print Post Top
Offlinenektar61S
Into SporePlay
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 18 hours
Re: Moldy basement SAB work (now with video) [Re: ModularMind]
    #27195992 - 02/09/21 05:46 PM (2 years, 11 months ago)

Quote:

ModularMind said:
Lightning




Ah. I'll try that. Duh. Why did I never think of that. That would help a lot. Thank you.

I thought it was something electrostatic, that would suck any airborne particles to the inside top of the SAB.

Wonder if that would work? It probably pull your spores up there too, and maybe make a tiny unwanted breeze.

I know you've been around here more than a decade longer than me, but I have a thought: I wonder if you can you make this work with a smaller weight on top? I am guessing that is contributing to the crack above the right hand hole, or will eventually.


--------------------
-NEW? Start here.


Extras: Filter Print Post Top
InvisibleModularMind
M.P.F.
Male User Gallery


Registered: 02/09/10
Posts: 7,902
Re: Moldy basement SAB work (now with video) [Re: nektar61]
    #27196828 - 02/10/21 06:26 AM (2 years, 11 months ago)

Now that you’ve quoted it, I realize I typo’d lighting.
I presume the weight is an effort to keep the box still if bumped. Not sure.


Extras: Filter Print Post Top
OfflineProfessor X
School for the Gifted
 User Gallery


Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
Re: Moldy basement SAB work [Re: ModularMind]
    #27197086 - 02/10/21 10:15 AM (2 years, 11 months ago)

I see two things, use a wire rack to Jack your plates up off the floor of the SAB, I use a small dish rack with the legs cut off. The other thing I see is you are turning over the recipient plates too much. Don't worry about the donor plate but keep the recipient upside down the entire time. Any plates I turned over like yours I would set to the side and Mark when I used a SAB, most of them failed. One last thing, I watched you sanitize then immediately put your hands over the plates, not good.

I would also make some "sleeves" I use dishwashing glove cuffs cut away from the hands, 2 sets to cover my forearms and iso everytime you move out of the box. Get a torch that stays lit too.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Bridgetown Botanicals Bridgetown Botanicals   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Microban plastic jars coconutjamushies 954 6 05/18/07 02:00 PM
by btronson
* microban Tomandjerry58 1,469 6 06/06/06 07:38 PM
by RogerRabbit
* impeller, evaporative, microban, filters, wicks, fans BUSTING A FCKING BLOODVESSEL HERE poof 1,326 6 08/24/07 05:25 PM
by poof
* 4 moldy jars boomerman 548 2 07/03/07 12:02 PM
by boomerman
* microban filter relativexistance 505 1 02/20/04 06:28 AM
by Prisoner#1
* moving into a moldy house :( not2sure 802 3 08/19/04 09:10 PM
by Fucknuckle
* Transfer to kowanite
( 1 2 3 all )
shroomben2003 3,159 45 08/01/07 02:44 AM
by UnderNose
* Moldy shrooms - will they grow? *DELETED* LAURinfamous 3,173 19 10/22/08 12:30 AM
by tripchip

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
999 topic views. 21 members, 183 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.013 seconds on 14 queries.