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OfflineSold Out Online
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AGAR: When to cut?
    #27000942 - 10/24/20 08:24 AM (4 years, 2 months ago)

This is a blue oyster biopsy that's about one week old. It has a nasty green roommate that become a bit of a nuisance, always borrowing things and never returning them.

My blue oyster is looking forward to having a home of its own one day, but he's not sure if he's strong enough to make it on his own yet. Should he wait a little while longer, grow bigger and stronger, and then move out? Or should he get out now before he himself becomes infected by little green goblin seeds?


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Invisiblealaskappalachian
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Re: AGAR: When to cut? [Re: Sold Out Online]
    #27001023 - 10/24/20 09:23 AM (4 years, 2 months ago)

Yeah you want to make transfers as soon as you have myc to grab.  Open the lid slowly and try not to disturb the dish much and grab a couple transfers asap.  The idea is to snag growth as quickly as you can to avoid contamination getting a foothold (and sporulating,  as it has in your dish).  Take a couple transfers and snag myc from them as soon as you get enough growth from them to take.  Hopefully you'll be able to transfer away from that.  Should be able to if you're careful. :thumbup:


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THE 49TH MYCOJOURNAL
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Re: AGAR: When to cut? [Re: alaskappalachian]
    #27001040 - 10/24/20 09:37 AM (4 years, 2 months ago)

I was hoping that was the answer, because I'm eager to try my first transfers!

From what I've read it seems like it shouldn't take much mycelium in a new plate to grow into a big, strong, strapping lad ready to knock up some grains and fill my belly again.

Cash money. Thanks for the fast response.


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Threads that have been useful to me:
Clean Spawn checklist for new growers

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Invisiblealaskappalachian
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Re: AGAR: When to cut? [Re: Sold Out Online]
    #27001095 - 10/24/20 10:49 AM (4 years, 2 months ago)

Absolutely.  Best of luck.  Nothing like fresh oysters.  Also- right you are on only needing a small transfer wedge.  Ideal = smaller.  Less surface area means less potential contaminants riding along. People take virtually invisible biopsies for LCs with needles so don't worry about going too small with your transfers.


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THE 49TH MYCOJOURNAL
Oyster Buckets

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OfflineShakedown Street
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Re: AGAR: When to cut? [Re: alaskappalachian]
    #27001564 - 10/24/20 04:52 PM (4 years, 2 months ago)

Wouldn't it be just as good to cut out the mold spot including several millimeters beyond where mold is visible ?

Edited by Shakedown Street (10/24/20 05:49 PM)

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Re: AGAR: When to cut? [Re: Shakedown Street]
    #27001572 - 10/24/20 04:59 PM (4 years, 2 months ago)

There’s an “always transfer AWAY from contamination” rule. I’m just paraphrasing what I’ve read in Paul Stamet’s Growing Gourmet and Medicinal Mushrooms, but I think it’s because it’s easier to get a clean piece out and into another dish than it is to know that you’ve actually cut all the contamination out.

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Offlineverum subsequentis
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Re: AGAR: When to cut? [Re: Sold Out Online]
    #27001581 - 10/24/20 05:07 PM (4 years, 2 months ago)

Mold spores are lighter than you think. It is best to leave them secured to the surface and held in place by gravity while taking a small transfer in a still air box. Picking up and moving the mold will just up your chances of failing.

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Re: AGAR: When to cut? [Re: verum subsequentis]
    #27001596 - 10/24/20 05:16 PM (4 years, 2 months ago)

verum, do mold spores eventually fall the the ground if there is no air flow in a room and nothing to lift or move them
Quote:

verum subsequentis said:
Mold spores are lighter than you think. It is best to leave them secured to the surface and held in place by gravity while taking a small transfer in a still air box. Picking up and moving the mold will just up your chances of failing.




verum, do mold spores eventually fall the the ground/floor if there is no air flow in a room and nothing to lift or move them?

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Offlineverum subsequentis
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Re: AGAR: When to cut? [Re: smalltalk_canceled]
    #27001610 - 10/24/20 05:25 PM (4 years, 2 months ago)

If they don't stick to something on the way down. Remember though, your concept of moving air is way different than a mold spore. Even slight temperature fluctuations cause movement of air. Not on a scale that can be noticed by us but enough to move spores around.

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Re: AGAR: When to cut? [Re: verum subsequentis]
    #27001623 - 10/24/20 05:36 PM (4 years, 2 months ago)

very informative. thank you. but you mentioning temperature makes me more curious

so stable temperature, no air movement - are mold spores remaining still?
i guess what im really wondering, is when are they still?

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Re: AGAR: When to cut? [Re: smalltalk_canceled]
    #27001646 - 10/24/20 05:52 PM (4 years, 2 months ago)

Very informative, cdnpsychonaught and verum subsequentis.  Now I am starting to understand.  Thank you.

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Re: AGAR: When to cut? [Re: Shakedown Street] * 1
    #27001880 - 10/24/20 08:55 PM (4 years, 2 months ago)

They are still anytime no external force is moving them. They do not move themselves.

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