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OfflineGrenik
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Lions Mane Agar Plates
    #27000533 - 10/23/20 09:33 PM (3 years, 3 months ago)

Looking at other posts here these do not look like contams, but they are not growing. These are the original plates from two liquid syringes (a Bears Head and a Lion's Mane). I marked one area I think I could get a transfer from. The rest...? I am growing on MEA.



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OfflineForresterM
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27000784 - 10/24/20 03:49 AM (3 years, 3 months ago)

I would try less nutritious agar.

That does look like mostly fruit on the americanum plate.  You could probably still transfer from it, it's all mycelium whether fruit or not, but what are you transferring to?  Would help to know what your goal is here.

They all do look clean to me though.

Hericium species are probably one of my favorites to watch grow as well hunt for.  I was lucky enough to find a TON of wild H. americanum this year, and a few H. coralloides as well, too bad I didn't have my setup to clone anything!


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OfflineGrenik
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Re: Lions Mane Agar Plates [Re: Forrester]
    #27001315 - 10/24/20 02:00 PM (3 years, 3 months ago)

Quote:

Forrester said:
Would help to know what your goal is here.





I am looking to grow them indoors in bags. So this was my first transfer from liquid. I am hoping to grow out a couple P1 or P2 plates and then make plates to put to grain and then bags.

Agar is Malt or Potato. Malt is 20 grams agar and 20 grams malt to 1300 mL of water. Pour 50 plates with a little left over.


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OfflineSyncrip
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27001341 - 10/24/20 02:22 PM (3 years, 3 months ago)

I have a Bear's Head plate going right now that was also straight from a liquid culture syringe. Here's some pictures for reference, they're growing on MYA (20g agar, 20g dry malt extract, 2g nutritional yeast, 1L water):



The picture on the left is the culture at 27 days old and on the right is a bit earlier at 23 days. The Bears head is probably the slowest growing of the gourmet cultures I have at the moment, and it definitely seems to like growing upwards rather than spreading outwards (in my hands anyways). Wish I had some earlier pictures saved but it didn't look too dissimilar to the picture you've posted.


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OfflineSHROOMSISAY01M
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27001345 - 10/24/20 02:24 PM (3 years, 3 months ago)

Is your agar even gelling with 1300 ml water? And It does not sound like you have enough malt extract either.

Here are the amounts I use...

1000 ml or 1000 g Distilled Water
22 grams of light malt extract
28 grams of agar

My agar was weak so I had to adjust it to 28 grams.


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OfflineGrenik
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Re: Lions Mane Agar Plates [Re: SHROOMSISAY01]
    #27001366 - 10/24/20 02:37 PM (3 years, 3 months ago)

I do not have any problems with it gelling. I boil on the stove before putting in jars and PC, so I lose some water. I probably have closer to 1250 mL when I pour. I think the "standard" is 20g agar to 1L water.

I use different suppliers for plates and one ships in 25 count sleeves and the other in 20 count. So I use the same amount of agar and malt for both, but add more water if I am going to pour 50 plates instead of 40. I have not noticed a difference when cutting wedges for agar-to-agar transfers.


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OfflineGrenik
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Re: Lions Mane Agar Plates [Re: Syncrip]
    #27001370 - 10/24/20 02:39 PM (3 years, 3 months ago)

Quote:

Syncrip said:
I have a Bear's Head plate going right now that was also straight from a liquid culture syringe. Here's some pictures for reference, they're growing on MYA (20g agar, 20g dry malt extract, 2g nutritional yeast, 1L water):



The picture on the left is the culture at 27 days old and on the right is a bit earlier at 23 days. The Bears head is probably the slowest growing of the gourmet cultures I have at the moment, and it definitely seems to like growing upwards rather than spreading outwards (in my hands anyways). Wish I had some earlier pictures saved but it didn't look too dissimilar to the picture you've posted.




What are your plans for the plates? Take them directly to grain or try to transfer and keep a master plate?


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OfflineSyncrip
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27001462 - 10/24/20 03:35 PM (3 years, 3 months ago)

I mostly just wanted to make sure I had a clean culture for when I eventually make up some slants and bank the variety in the fridge for later. I may have impulsively ordered more cultures than I know what to do with recently... so my main priority right now is just getting everything cleaned and stored so I don't lose any of them.

I'll probably make a transfer and keep an agar plate going as well just for fun though. It's been growing slow enough that it's not going to be much extra work or cost.

I've been going agar -> LC -> grains for my grows so far.


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OfflineForresterM
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Re: Lions Mane Agar Plates [Re: Grenik] * 1
    #27001493 - 10/24/20 04:02 PM (3 years, 3 months ago)

Quote:

Grenik said:
I am looking to grow them indoors in bags. So this was my first transfer from liquid. I am hoping to grow out a couple P1 or P2 plates and then make plates to put to grain and then bags.





If it were me I'd probably just work from the LC as it appears to be clean.  Sometimes it can be hard to get hericium not to fruit on plates... but those are good to transfer or slant or whatever you wanna do with em.


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Invisibleteknix
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27002760 - 10/25/20 01:28 PM (3 years, 3 months ago)

Was the LC clear? It could have added nutrients from that vector as well.


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Invisibleteknix
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Re: Lions Mane Agar Plates [Re: teknix]
    #27002807 - 10/25/20 01:55 PM (3 years, 3 months ago)



Mine kinda did the same thing, not as bad, but I had used activated carbon in this dish too, if that makes any difference.


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OfflineKuda1988
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Re: Lions Mane Agar Plates [Re: Syncrip]
    #27911573 - 08/20/22 05:02 PM (1 year, 5 months ago)

Quote:

Syncrip said:
I mostly just wanted to make sure I had a clean culture for when I eventually make up some slants and bank the variety in the fridge for later. I may have impulsively ordered more cultures than I know what to do with recently... so my main priority right now is just getting everything cleaned and stored so I don't lose any of them.

I'll probably make a transfer and keep an agar plate going as well just for fun though. It's been growing slow enough that it's not going to be much extra work or cost.

I've been going agar -> LC -> grains for my grows so far.




I’m a newb to lions mane , currently gathering agar from SS, transferring till I get a couple nice slants.

Do you have to make a LC or can I just drop agar wedges in sterilized grain bags ?


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OfflineForresterM
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27911982 - 08/21/22 01:15 AM (1 year, 5 months ago)

You can just do wedges to grain, that's probably what most do.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineBismillah
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Re: Lions Mane Agar Plates [Re: Forrester]
    #27943822 - 09/10/22 02:16 PM (1 year, 4 months ago)

I cloned a lion's mane 17 days ago. Does this look good?


I have two more transfers that look the same. Dog food agar.

Should I transfer to grain now, or can the plates wait a bit? I'm not quite ready to start growing yet. Fridge perhaps?


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OfflineForresterM
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Re: Lions Mane Agar Plates [Re: Grenik] * 1
    #27943923 - 09/10/22 03:37 PM (1 year, 4 months ago)

Looks about right, fridge should be ok to slow em down a bit and maybe keep from fruiting


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineEyeof
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Re: Lions Mane Agar Plates [Re: Forrester]
    #27945341 - 09/11/22 01:16 PM (1 year, 4 months ago)

From that clone pic, which sector would you grab samples from or try to spawn (to grain??)


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OfflineForresterM
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Re: Lions Mane Agar Plates [Re: Grenik]
    #27946176 - 09/12/22 12:06 AM (1 year, 4 months ago)

Since it's a clone not spores, it doesn't really matter, transfer from wherever it looks cleanest it should all be the same genetics


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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