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OfflineAlexjones
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Registered: 07/29/20
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Agar to brf question
    #26999974 - 10/23/20 03:47 PM (3 years, 3 months ago)

So I have a few agar plates going, I was reading about if you use a wedge to inoculate a brf jar you need to have a lid with a special kind of GE hole on it? The lids I have now have either 4 or 2 holes I had previously used to inoculate with a syringe, would it be a problem if I used these or should I get new lids?


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OfflineOne of Us
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Re: Agar to brf question [Re: Alexjones]
    #27000003 - 10/23/20 04:06 PM (3 years, 3 months ago)

I think they would work fine with a 2-3 layers of micropore tape over the holes. I'm assuming you won't be using a verm layer, right? And no PC?


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OfflineAlexjones
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Re: Agar to brf question [Re: One of Us]
    #27000040 - 10/23/20 04:32 PM (3 years, 3 months ago)

Yeah no Verm layer and yes I’m gonna pressure cook them at 15 psi to sterilize before inoculation with the agar wedges. I would do oats but it seems like it takes a lot of work to prep the grains and everything


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OfflineDnDRnD
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Re: Agar to brf question [Re: Alexjones] * 1
    #27000048 - 10/23/20 04:38 PM (3 years, 3 months ago)

If you already have clean agar then grains is always the way to go, oats are very easy to prep and theres even a few no prep teks out there

For agar to BRF though you can fill a syringe with distilled water and sterilize it in the PC then just poke the agar puck with the syringe and squirt a small drop of water out to dislodge the agar into the BRF

Check out Josex's stick and poke method for a more detailed description but it's basically the same thing except in his method you go from mushroom to LC or agar to LC


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OfflineOne of Us
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Re: Agar to brf question [Re: Alexjones]
    #27000055 - 10/23/20 04:41 PM (3 years, 3 months ago)

Oats are super easy. I just boil them for 40-45mins and pour into a strainer. Then I just leave em there overnight, then they're fine to jar up and pc anytime that day. I wouldn't leave them out more than 24 hours though.


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Invisiblealaskappalachian
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Re: Agar to brf question [Re: Alexjones]
    #27000062 - 10/23/20 04:45 PM (3 years, 3 months ago)

Takes less work to do oats than brf in volume really (as mentioned while I was bungling  around typing this),  but I'm not trying to steer you away from brf.  Plus you can only go so big with brf/verm or it takes forever.  You could  a. Just drop a wedge in and use mpg tape on the holes as mentioned above,  or b. take that old syringe,  and flush it with rapidly boiling water a few times,  retaining the final draw), and either squirt it into the agar dish (if it's a prepour) or a sterile jar with a big wedge (if it's a petri) and make a LI to noc up brf jars like usual.  You'll get way, way faster growth with that method and you can do more jars with less contamination vectors.  Still faster to do oats but that'll keep it in brf context for ya.


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OfflineAlexjones
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Re: Agar to brf question [Re: alaskappalachian]
    #27000081 - 10/23/20 04:57 PM (3 years, 3 months ago)

Guess I will hold off on it until I get some oats and full pint jars, I got a clone I’m working on but the plate I transferred it from had some bacteria on it hopefully I can get that cleaned up


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Offlinetimothywyllie
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Re: Agar to brf question [Re: One of Us]
    #27604107 - 01/02/22 04:10 PM (2 years, 26 days ago)

Quote:

One of Us said:
Oats are super easy. I just boil them for 40-45mins and pour into a strainer. Then I just leave em there overnight, then they're fine to jar up and pc anytime that day. I wouldn't leave them out more than 24 hours though.




Does boiling them that long not result in a lot of burst grains?


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OfflineForresterM
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Re: Agar to brf question [Re: timothywyllie]
    #27604141 - 01/02/22 04:38 PM (2 years, 26 days ago)

Quote:

Alexjones said:
Guess I will hold off on it until I get some oats and full pint jars, I got a clone I’m working on but the plate I transferred it from had some bacteria on it hopefully I can get that cleaned up




Might as well go with quart jars if you're making grain spawn anyway.  Same amount of work, twice the spawn.


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Invisiblefungusul
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Registered: 07/16/20
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Re: Agar to brf question [Re: Forrester]
    #27605210 - 01/03/22 01:47 PM (2 years, 25 days ago)

For agar to BRF, you can convert your PF-TEK lids to Dawgy style lids.

Just add 2 layers of micropore tape on existing holes(1/8") and use a heavy synthetic felt 4mm+(polypropylene or polyester stiff felt). To prevent any FAE issues, you might need to enlarge existing holes  to be at least 1/8".

The same lid setup can be used for grains.


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