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Offlinejaydeezy817
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Is this contamination
    #26999323 - 10/23/20 08:53 AM (4 years, 2 months ago)

Im ao bad at agar. Is this contamination or what. Thanks

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OfflineFoTwenty
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Re: Is this contamination [Re: jaydeezy817]
    #26999326 - 10/23/20 08:55 AM (4 years, 2 months ago)

Yes


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Offlinetiptrippy
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Re: Is this contamination [Re: FoTwenty]
    #26999386 - 10/23/20 09:41 AM (4 years, 2 months ago)

Yes it is contamination but that is ok. That is the whole point of using agar. You can take a small slice of healthy mycelium from that dish and transfer it to another dish. Keep doing so until you get only clean and healthy growth.

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InvisibleNichrome
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Re: Is this contamination [Re: tiptrippy]
    #26999432 - 10/23/20 10:24 AM (4 years, 2 months ago)

That's bad. Looks like E. Coli. I clean up a lot of cultures infected with a lot of different things and personally I'd throw those strait in the trash and start over. Sorry. Luckily spores are cheap or free.


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InvisibleRoger Clemency
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Re: Is this contamination [Re: FoTwenty]
    #26999518 - 10/23/20 11:09 AM (4 years, 2 months ago)

I’ve never used those little ketchup cups but the times I’ve tried No Pour using little Tupperware containers I hated it. With Petri dishes it’s so simple to just lift the lid off one handed and take a wedge without hovering your hand over top of the plate since they have short walls.

I can’t say how the bacteria got there for sure but the less smooth things go the better the chance of baddies getting in


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Heaven starts right where hell ends

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Offlinejaydeezy817
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Re: Is this contamination [Re: Roger Clemency]
    #26999935 - 10/23/20 03:23 PM (4 years, 2 months ago)

Damn thanks guys very informative. Agar has been kicking my ass for years. Should i completely sterilize in the pc for 2 hours at 15 psi? Because i only did it for 30 minutes

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OfflineFoTwenty
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Re: Is this contamination [Re: jaydeezy817]
    #27000016 - 10/23/20 04:14 PM (4 years, 2 months ago)

How are you cooling down the pc?
SAB?
Temperature difference creates a vacuum when cooling down. Needs to be clean air. Or put a SFD on the lid of the agar bottle.
I Assume that you don’t Screw it tight...


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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