Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Offlinedebord
Stranger
 User Gallery
Registered: 01/26/18
Posts: 97
Last seen: 10 months, 22 days
1st Time Agar - Help!
    #26999182 - 10/23/20 06:41 AM (3 years, 3 months ago)

Hello.

I bought some Agar plates online (this could be the problem!).
They were Mueller Hinton Agar, my thinking is perhaps these are wrong and there are no nutrients, or not the right ones, in there?

It's been a week and nothing seems to be happening.
My current thinking is start again and get some new clean agar mycellium...
I've probably messed them up by getting them out of the ziplock bag there were in too! I used cling film over them because I couldn't work out how to make the parafilm work. This is all deeply embarrassing







Any help or advice would be great


Extras: Filter Print Post Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
 Unread Journal


Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: 1st Time Agar - Help! [Re: debord]
    #26999268 - 10/23/20 08:04 AM (3 years, 3 months ago)

Plates don't take up much space and are fun to watch develop. Definetly start up some new plates, you can't have too many imo. But hang on to these. Someone with more experience will weigh in prehaps.

What's up with the dark colour on the wedges? Were they like that from the start?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


Extras: Filter Print Post Top
Offlinedebord
Stranger
 User Gallery
Registered: 01/26/18
Posts: 97
Last seen: 10 months, 22 days
Re: 1st Time Agar - Help! [Re: coversall]
    #26999316 - 10/23/20 08:49 AM (3 years, 3 months ago)

Thanks for the reply.

The wedges were pale but now they have gone that dark colour, which is also ominous!


Extras: Filter Print Post Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
 Unread Journal


Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: 1st Time Agar - Help! [Re: debord]
    #26999325 - 10/23/20 08:55 AM (3 years, 3 months ago)

Yeah that doesn't seem super promising to me. But like I said, isolate them from clean plates, watch them and take notes. Have you read the various agar guide threads?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


Extras: Filter Print Post Top
OfflineSingularFusion
I'm a teapot User Gallery

Registered: 10/31/18
Posts: 1,208
Last seen: 7 hours, 52 minutes
Re: 1st Time Agar - Help! [Re: coversall]
    #26999338 - 10/23/20 09:03 AM (3 years, 3 months ago)

yeah that agar is definitely wrong for mush, it's blood based from what I could read, from wiki:

''Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing. It is also used to isolate and maintain Neisseria and Moraxella species. It typically contains: • 2.0 g beef extract • 17.5 g casein hydrolysate • 1.5 g starch • 17.0 g agar • 1 liter of distilled water. • pH adjusted to neutral at 25 °C. Five percent sheep blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus species.''

but if you make some of your own plates you could maybe save something from there with some transfers if that is all you have

otherwise start again with your own plates and spores, simple MEA or PDA works great in my experience

good luck


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Extras: Filter Print Post Top
Offlinedebord
Stranger
 User Gallery
Registered: 01/26/18
Posts: 97
Last seen: 10 months, 22 days
Re: 1st Time Agar - Help! [Re: SingularFusion]
    #27005797 - 10/27/20 07:10 AM (3 years, 3 months ago)

Thank you for the advice, very helpful.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* my 1st agar petri lepiota 1,387 13 06/09/05 03:54 PM
by Hotnuts
* What to do with 1st agar plate fromthemoon 1,382 3 09/07/06 03:20 PM
by monstermitch
* buying agar - help sinisha 3,416 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,156 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,135 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 4,589 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,650 4 03/01/06 09:08 AM
by Dem_Bones

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
193 topic views. 28 members, 274 guests and 70 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.