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debord
Stranger

Registered: 01/26/18
Posts: 97
Last seen: 10 months, 22 days
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1st Time Agar - Help!
#26999182 - 10/23/20 06:41 AM (3 years, 3 months ago) |
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Hello.
I bought some Agar plates online (this could be the problem!). They were Mueller Hinton Agar, my thinking is perhaps these are wrong and there are no nutrients, or not the right ones, in there?
It's been a week and nothing seems to be happening. My current thinking is start again and get some new clean agar mycellium... I've probably messed them up by getting them out of the ziplock bag there were in too! I used cling film over them because I couldn't work out how to make the parafilm work. This is all deeply embarrassing



Any help or advice would be great
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: 1st Time Agar - Help! [Re: debord]
#26999268 - 10/23/20 08:04 AM (3 years, 3 months ago) |
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Plates don't take up much space and are fun to watch develop. Definetly start up some new plates, you can't have too many imo. But hang on to these. Someone with more experience will weigh in prehaps.
What's up with the dark colour on the wedges? Were they like that from the start?
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debord
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Registered: 01/26/18
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Last seen: 10 months, 22 days
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Re: 1st Time Agar - Help! [Re: coversall]
#26999316 - 10/23/20 08:49 AM (3 years, 3 months ago) |
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Thanks for the reply.
The wedges were pale but now they have gone that dark colour, which is also ominous!
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: 1st Time Agar - Help! [Re: debord]
#26999325 - 10/23/20 08:55 AM (3 years, 3 months ago) |
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Yeah that doesn't seem super promising to me. But like I said, isolate them from clean plates, watch them and take notes. Have you read the various agar guide threads?
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SingularFusion


Registered: 10/31/18
Posts: 1,208
Last seen: 7 hours, 52 minutes
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Re: 1st Time Agar - Help! [Re: coversall]
#26999338 - 10/23/20 09:03 AM (3 years, 3 months ago) |
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yeah that agar is definitely wrong for mush, it's blood based from what I could read, from wiki:
''Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing. It is also used to isolate and maintain Neisseria and Moraxella species. It typically contains: • 2.0 g beef extract • 17.5 g casein hydrolysate • 1.5 g starch • 17.0 g agar • 1 liter of distilled water. • pH adjusted to neutral at 25 °C. Five percent sheep blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus species.''
but if you make some of your own plates you could maybe save something from there with some transfers if that is all you have
otherwise start again with your own plates and spores, simple MEA or PDA works great in my experience
good luck
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debord
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Registered: 01/26/18
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Last seen: 10 months, 22 days
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Thank you for the advice, very helpful.
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