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MikeLitoris
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World’s Biggest BRF Cake? 3
#26996717 - 10/21/20 04:55 PM (3 years, 3 months ago) |
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 Made a large BRF cake that I’m planning to inoculating with liquid culture. Pint jar for reference.
8 cups of vermiculite 4 cups of BRF 4 Cups of water
I have a 10 inch long needle that should get the LC to the edges of the jar.
How much LC do you guys think I should use? I should be able to get a pretty thick LC by putting the long needle directly into the mycelium pile at the bottom of the LC.
-------------------- Weapons not food, not homes, not shoes Not need, just feed the war cannibal animal I walk tha corner to tha rubble that used to be a library Line up to tha mind cemetery now What we don't know keeps tha contracts alive an movin' They don't gotta burn tha books they just remove 'em While arms warehouses fill as quick as tha cells Rally round tha family, pockets full of shells
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edgar1337
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Re: World’s Biggest BRF Cake? [Re: MikeLitoris]
#26996906 - 10/21/20 07:31 PM (3 years, 3 months ago) |
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I've been told by experts on here that less is more - you don't need much spore liquid for it to take. The most common is 1cc per jar, some recommend using more for larger jars. I'm not sure how experienced you are, but that BRF jar looks strange. I don't see a dry verm. layer on top, but I guess its not necessary with gas exchange filters.
Definitely an unorthodox jar. I'm not experienced enough to give advise, but if it was my jar, I'd put 1cc/ml total.
-------------------- "Mistakes are, after all, the foundations of truth, and if a man does not know what a thing is, it is at least an increase in knowledge if he knows what it is not." -Carl Jung "The current state of knowledge is a moment in history, changing just as rapidly as the state of knowledge in the past has ever changed and, in many instances, more rapidly." -Jean Piaget
Edited by edgar1337 (10/21/20 07:32 PM)
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BLINKfan420
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Re: World’s Biggest BRF Cake? [Re: edgar1337]
#26996910 - 10/21/20 07:33 PM (3 years, 3 months ago) |
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Oh boy, may the mushrooms gods smile upon you and not let that thing stall. 😬🤞
BLiNK
--------------------
  All my pictures are drawn from imagination. I don't even know what a mushroom is.
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tripdawg420
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Re: World’s Biggest BRF Cake? [Re: BLINKfan420]
#26996922 - 10/21/20 07:47 PM (3 years, 3 months ago) |
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MikeLitoris
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Re: World’s Biggest BRF Cake? [Re: BLINKfan420]
#26997017 - 10/21/20 08:55 PM (3 years, 3 months ago) |
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Quote:
BLINKfan420 said: Oh boy, may the mushrooms gods smile upon you and not let that thing stall. 😬🤞
BLiNK
I’m going to inoculate with a very dense LC and hope that works out. If not, I have some grains that are colonizing too. This isn’t my only experiment I have going right now.
-------------------- Weapons not food, not homes, not shoes Not need, just feed the war cannibal animal I walk tha corner to tha rubble that used to be a library Line up to tha mind cemetery now What we don't know keeps tha contracts alive an movin' They don't gotta burn tha books they just remove 'em While arms warehouses fill as quick as tha cells Rally round tha family, pockets full of shells
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MikeLitoris
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Re: World’s Biggest BRF Cake? [Re: edgar1337]
#26997025 - 10/21/20 08:58 PM (3 years, 3 months ago) |
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Quote:
edgar1337 said: I've been told by experts on here that less is more - you don't need much spore liquid for it to take. The most common is 1cc per jar, some recommend using more for larger jars. I'm not sure how experienced you are, but that BRF jar looks strange. I don't see a dry verm. layer on top, but I guess its not necessary with gas exchange filters.
Definitely an unorthodox jar. I'm not experienced enough to give advise, but if it was my jar, I'd put 1cc/ml total.
It’s a storage jar that is rated to 400F. You can put it in the oven. I picked that jar because it has a wide mouth and I can get the cake out when it’s time to dunk and roll.
I didn’t put a dry vermouth layer because it has the filter disc in place. I’ll keep everyone posted On the progress of this experiment.
-------------------- Weapons not food, not homes, not shoes Not need, just feed the war cannibal animal I walk tha corner to tha rubble that used to be a library Line up to tha mind cemetery now What we don't know keeps tha contracts alive an movin' They don't gotta burn tha books they just remove 'em While arms warehouses fill as quick as tha cells Rally round tha family, pockets full of shells
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adhoc


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Re: World’s Biggest BRF Cake? [Re: MikeLitoris] 3
#26998364 - 10/22/20 05:21 PM (3 years, 3 months ago) |
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Quote:
MikeLitoris said: I didn’t put a dry vermouth layer
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Wall.E
Bacteria's Bitch



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Posts: 2,860
Loc: Fungal Void
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Re: World’s Biggest BRF Cake? [Re: adhoc]
#26998480 - 10/22/20 06:38 PM (3 years, 3 months ago) |
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Fuck it, shoot 10 ml into it why not
-------------------- Life’s shit, but I’m loving it
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edgar1337
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Re: World’s Biggest BRF Cake? [Re: MikeLitoris]
#26998868 - 10/22/20 11:22 PM (3 years, 3 months ago) |
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Quote:
MikeLitoris said:
Quote:
edgar1337 said: I've been told by experts on here that less is more - you don't need much spore liquid for it to take. The most common is 1cc per jar, some recommend using more for larger jars. I'm not sure how experienced you are, but that BRF jar looks strange. I don't see a dry verm. layer on top, but I guess its not necessary with gas exchange filters.
Definitely an unorthodox jar. I'm not experienced enough to give advise, but if it was my jar, I'd put 1cc/ml total.
It’s a storage jar that is rated to 400F. You can put it in the oven. I picked that jar because it has a wide mouth and I can get the cake out when it’s time to dunk and roll.
I didn’t put a dry vermouth layer because it has the filter disc in place. I’ll keep everyone posted On the progress of this experiment.
It looks pretty fancy, but probably too big to fit in anything but the larger pressure cookers. Its neat that you can put it in the oven. But honestly, I'm not sure if that will sterilize it. I've been advised on here that PC/boiling water sterilizes by steam, one person called it fractional sterilization.
I've never seen a tek that uses oven sterilizing, but there is a first for everything. Hopefully you have a big enough pot for that monster. Good luck! The largest I've tried is 16oz/1 pint jar, and I was advised on here to always use more smaller jars vs less larger ones. If something goes wrong, you lose the whole thing. An anomaly/error on smaller jars will kill one jar of many. One guy explained that it was like a "race" between the mycellium and other potential competing bacteria/contaminations. The longer it takes to colonize, the higher the chance it will die from contams.
In my experience using more spore liquid results in faster colonization (but its tricky to avoid too much moisture), so you might want to use extra and hope it will outpace anything else that could be in there. If it turns out oven sterilization works, that could be a big breakthrough in lowering the threshold for equipment needed to cultivate.
Edited by edgar1337 (10/22/20 11:25 PM)
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
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Re: World’s Biggest BRF Cake? [Re: Wall.E]
#26999251 - 10/23/20 07:47 AM (3 years, 3 months ago) |
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Quote:
Myc_Hunt said: Fuck it, shoot 10 ml into it why not
My first BRF cake I didn't really know how much liquid I was supposed to use, so, BAM 14ml into one 360ml cake!
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MikeLitoris
Stranger


Registered: 07/16/20
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Loc: Minnesota
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Re: World’s Biggest BRF Cake? [Re: edgar1337]
#27000675 - 10/24/20 12:00 AM (3 years, 3 months ago) |
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Quote:
edgar1337 said:
Quote:
MikeLitoris said:
Quote:
edgar1337 said: I've been told by experts on here that less is more - you don't need much spore liquid for it to take. The most common is 1cc per jar, some recommend using more for larger jars. I'm not sure how experienced you are, but that BRF jar looks strange. I don't see a dry verm. layer on top, but I guess its not necessary with gas exchange filters.
Definitely an unorthodox jar. I'm not experienced enough to give advise, but if it was my jar, I'd put 1cc/ml total.
It’s a storage jar that is rated to 400F. You can put it in the oven. I picked that jar because it has a wide mouth and I can get the cake out when it’s time to dunk and roll.
I didn’t put a dry vermouth layer because it has the filter disc in place. I’ll keep everyone posted On the progress of this experiment.
It looks pretty fancy, but probably too big to fit in anything but the larger pressure cookers. Its neat that you can put it in the oven. But honestly, I'm not sure if that will sterilize it. I've been advised on here that PC/boiling water sterilizes by steam, one person called it fractional sterilization.
I've never seen a tek that uses oven sterilizing, but there is a first for everything. Hopefully you have a big enough pot for that monster. Good luck! The largest I've tried is 16oz/1 pint jar, and I was advised on here to always use more smaller jars vs less larger ones. If something goes wrong, you lose the whole thing. An anomaly/error on smaller jars will kill one jar of many. One guy explained that it was like a "race" between the mycellium and other potential competing bacteria/contaminations. The longer it takes to colonize, the higher the chance it will die from contams.
In my experience using more spore liquid results in faster colonization (but its tricky to avoid too much moisture), so you might want to use extra and hope it will outpace anything else that could be in there. If it turns out oven sterilization works, that could be a big breakthrough in lowering the threshold for equipment needed to cultivate.
I put it in my Pressure Cooker and steamed it for 90 minutes.
-------------------- Weapons not food, not homes, not shoes Not need, just feed the war cannibal animal I walk tha corner to tha rubble that used to be a library Line up to tha mind cemetery now What we don't know keeps tha contracts alive an movin' They don't gotta burn tha books they just remove 'em While arms warehouses fill as quick as tha cells Rally round tha family, pockets full of shells
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MikeLitoris
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Re: World’s Biggest BRF Cake? [Re: adhoc]
#27000678 - 10/24/20 12:03 AM (3 years, 3 months ago) |
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There’s a synthetic filter disc on the lid and 4 self-healing injection ports. 🤷🏽♂️
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Rikazi
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Re: World’s Biggest BRF Cake? [Re: MikeLitoris]
#27000694 - 10/24/20 12:19 AM (3 years, 3 months ago) |
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Large BRF jars do not do well. The colonization is super slow and your chance of contamination is greater. I tried pint and a half jars with Brf a long time ago and it was a disaster.
If you want to go big... just start doing grains and monotubs/minimonos
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MikeLitoris
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Re: World’s Biggest BRF Cake? [Re: Rikazi]
#27000890 - 10/24/20 07:29 AM (3 years, 3 months ago) |
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Quote:
Rikazi said: Large BRF jars do not do well. The colonization is super slow and your chance of contamination is greater. I tried pint and a half jars with Brf a long time ago and it was a disaster.
If you want to go big... just start doing grains and monotubs/minimonos
I have grains going too. I’m just messing around with different TEK.
Anyway, I ordered 10 inch long needles from Amazon and they sent me a pair of women’s socks ffs.
-------------------- Weapons not food, not homes, not shoes Not need, just feed the war cannibal animal I walk tha corner to tha rubble that used to be a library Line up to tha mind cemetery now What we don't know keeps tha contracts alive an movin' They don't gotta burn tha books they just remove 'em While arms warehouses fill as quick as tha cells Rally round tha family, pockets full of shells
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PBJ710
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Re: World’s Biggest BRF Cake? [Re: Rikazi]
#27000894 - 10/24/20 07:32 AM (3 years, 3 months ago) |
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I would be concerned about the middle reaching temp in an oven as well. At 15psi, I would want to PC it for 4 hours - with just dry heat, it might take excessively long to reach temp in the middle.
I don't think colonization will be slow if he's using a decent LC (like the OP is doing). He'll be innoculating them through the SHIPs, so contam vectors are actually probably lower than standard PFTek. The SHIPs seal up as soon as you pull the syringe out vs. covering the hole after the inoculation...
Onward with the science! Lets see how it turns out.
Edited by PBJ710 (10/24/20 07:39 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
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Re: World’s Biggest BRF Cake? [Re: PBJ710]
#27000960 - 10/24/20 08:35 AM (3 years, 3 months ago) |
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I would use as much LC as possible, try and get it to run to the bottom of the jar if possible. You are going to be fighting time and gravity on this one so you need to get the bottom colonized ASAP or the whole thing will likely be compacted in a week.
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Mateja


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Re: World’s Biggest BRF Cake? [Re: Pastywhyte]
#27001016 - 10/24/20 09:20 AM (3 years, 3 months ago) |
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Quote:
MikeLitoris said:
 Made a large BRF cake that I’m planning to inoculating with liquid culture. Pint jar for reference.
8 cups of vermiculite 4 cups of BRF 4 Cups of water
I have a 10 inch long needle that should get the LC to the edges of the jar.
How much LC do you guys think I should use? I should be able to get a pretty thick LC by putting the long needle directly into the mycelium pile at the bottom of the LC.
I'd say which ever amount of LC you chose to inject make sure to spread it around as much as you can. Try to shower as much of the BRF mix as you can, when I do cakes I start injecting as the needle is tilted 45degrees in one direction along the glass and I inject continuously as I swipe the needle all the way to the opposite side 45degree angle (hope that makes sense).
Quote:
Pastywhyte said I would use as much LC as possible
Yeah 4 tiny specs of inoculation in a BRF substrate of that size will undoubtedly not colonize properly at all. But then again I personally don't feel that a BRF/Verm mix is optimal terrain for colonizing myc. I've rarely been able to get 1/2 pint BRF cakes to colonize in under a month with LI and LC. But 2 parts hydrated coir and 1 part oatmeal is so far the best/fastest (most airy) substrate I've made. This is 9 day colonization using 5ml of LC.
-------------------- Cakes inside Water Tub
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MikeLitoris
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Re: World’s Biggest BRF Cake? [Re: Pastywhyte] 1
#27005752 - 10/27/20 06:15 AM (3 years, 3 months ago) |
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Quote:
Pastywhyte said: I would use as much LC as possible, try and get it to run to the bottom of the jar if possible. You are going to be fighting time and gravity on this one so you need to get the bottom colonized ASAP or the whole thing will likely be compacted in a week.
I inoculated it with 60cc of very concentrated LC and a 10 inch blunt tip needle. I was able to get all the way to the bottom of the jar and injected LC at the very bottom and slowly as I pulled the needle out of each injection port.
It is now in my incubator at 78° F.
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One of Us
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Re: World’s Biggest BRF Cake? [Re: MikeLitoris]
#27005763 - 10/27/20 06:38 AM (3 years, 3 months ago) |
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MikeLitoris
Stranger


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Re: World’s Biggest BRF Cake? [Re: One of Us] 2
#27008335 - 10/28/20 12:58 PM (3 years, 2 months ago) |
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Day # 2 after inoculating
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