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mov369


Registered: 03/12/20
Posts: 25
Loc: Earth
Last seen: 2 months, 18 days
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Selecting for better fruits
#26995078 - 10/20/20 06:03 PM (3 years, 3 months ago) |
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Hi,
I have a question about selecting for a faster colonising/better fruiting strain.
How do you guys do it? Do you make a decision based on the thread thickness and definition on the agar petri dishes? Do you just take sporeprints of mushrooms you think look sexy?
I have quite a few petri dishes, some of them are 3rd transfers (I was selecting for girthier, thicker and more unified threads on the periphery of myc growth). Am I doing it right? Will this selection lead to any good?
From what I understand ideal is a single strain isolate (as if completely uniform circular growth with girthy threads) that is then cloned onto many petris and used to inoculate spawn. Please correct me if I'm wrong.
Thank you!
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 7 hours
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Re: Selecting for better fruits [Re: mov369]
#26995114 - 10/20/20 06:25 PM (3 years, 3 months ago) |
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Other more experienced posters will probably fill out more, but I know that we select for colonization speed and perceived or tested potency of the fruits.
For instance, if a grain transfer has especially fast growth compared to others, this helps qualify it for a grain master. Transfers from this may produce a row of fast colonizing jars.
If the same jars fruit well and the fruits have average to high perceived active content, looks good, have interesting traits or come up in a big healthy cluster, we have reason to clone it.
The clone is then taken to agar, perhaps cleaned up, and then used to knock up new jars. You may also end up choosing a new grain maste from those, fruit from those, clone again etc.
It seems also that the Shroomery "Meta" today has become "clone, dont isolate on dishes". As in that its considered not very efficient to spend time on genetic isolation vs just cloning good fruits from exellent clusters.
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (10/20/20 06:29 PM)
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mov369


Registered: 03/12/20
Posts: 25
Loc: Earth
Last seen: 2 months, 18 days
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Thanks! I'll have to look into cloning then. Judging by that horse-cock of a mushroom you got in your sig you know what you're talking about
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Big_Dub
I'm just some guy



Registered: 01/12/11
Posts: 2,640
Loc: Los Angeles
Last seen: 42 minutes, 33 seconds
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Re: Selecting for better fruits [Re: mov369]
#26995137 - 10/20/20 06:41 PM (3 years, 3 months ago) |
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you want to start cloning nice looking fruits using agar.
-------------------- split_by_nine said: click me you fuck do the right thing
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 13 days, 24 minutes
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Re: Selecting for better fruits [Re: Big_Dub]
#26995151 - 10/20/20 06:52 PM (3 years, 3 months ago) |
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Selecting for better fruits [Re: Inthepit]
#26995208 - 10/20/20 07:17 PM (3 years, 3 months ago) |
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Just clone nice fruits. When I dont get a nice fruit from a culture after a few tries, I find its quicker to just go back to the print and start a new culture from spore than to keep fucking around with what I got. YMMV
Getting a clone culture going is key to making everything smooth and consistent. I like to only fuck with spores like once a year lol.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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