Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post Pages: 1
Offlinepitriot
Stranger
 User Gallery

Registered: 06/16/19
Posts: 455
Last seen: 3 months, 4 days
Using LC ?
    #26994967 - 10/20/20 04:58 PM (3 years, 3 months ago)

I was trying to suck up some LC and it jammed the needle. It was an 18g needle. I tried a couple with same results.
I am not sure if this is the reason people talk about breaking up the culture in the bottle or do I need a larger needle?
If the former should I get a magnetic stirrer, would that solve this kind of problem?
If the latter what size needle would be appropriate?


Extras: Filter Print Post Top
Offlinetrippleblack
Stranger
 User Gallery
Registered: 12/01/19
Posts: 355
Last seen: 22 hours, 19 minutes
Re: Using LC ? [Re: pitriot]
    #26995011 - 10/20/20 05:21 PM (3 years, 3 months ago)

just push plunger back in while needle is in liquid culture jar to clear needle and suck the liquid back up again..

this happens time to time..


Extras: Filter Print Post Top
OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 21 minutes
Trusted Cultivator
Re: Using LC ? [Re: pitriot]
    #26995019 - 10/20/20 05:25 PM (3 years, 3 months ago)

Dispensing needles are what you want if you use needles for lc. You can also just pour some from one vessel into another and skip the needles. That takes some practice.

Check these out.
https://www.amazon.com/Dispense-All-Dispensing-Needle-Stainless/dp/B07995JTHN

They come in lots of sizes.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


Extras: Filter Print Post Top
Offlinepitriot
Stranger
 User Gallery

Registered: 06/16/19
Posts: 455
Last seen: 3 months, 4 days
Re: Using LC ? [Re: Nichrome]
    #26995331 - 10/20/20 08:20 PM (3 years, 3 months ago)

I like these dispensing needles. I like that they are stainless steel and easy to sterilize. But I'm wondering if 8g is not too large for self healing ports? Maybe to get 12g or 14g?


Extras: Filter Print Post Top
Offlinepitriot
Stranger
 User Gallery

Registered: 06/16/19
Posts: 455
Last seen: 3 months, 4 days
Re: Using LC ? [Re: trippleblack]
    #26995334 - 10/20/20 08:21 PM (3 years, 3 months ago)

I had done this multiple times and didn't succeed. It is especially difficult working as I am in a SAB.


Extras: Filter Print Post Top
Offlinenmd_myco
Obsessed Noob
 User Gallery

Registered: 09/05/20
Posts: 198
Loc: PNW
Last seen: 1 year, 4 months
Re: Using LC ? [Re: pitriot]
    #26995400 - 10/20/20 08:54 PM (3 years, 3 months ago)

LC in an SAB. That's gangster.


Extras: Filter Print Post Top
OfflineNichrome
I'm a torso!
I'm a teapot User Gallery


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 21 minutes
Trusted Cultivator
Re: Using LC ? [Re: pitriot]
    #26995469 - 10/20/20 09:56 PM (3 years, 3 months ago)

Quote:

pitriot said:
I like these dispensing needles. I like that they are stainless steel and easy to sterilize. But I'm wondering if 8g is not too large for self healing ports? Maybe to get 12g or 14g?




Oh yeah way too big for ports. If you're comfortable with your space just pop the lids open quickly and squirt some right in the jar. Lots of folks don't use ports. You can do LC in an SAB just fine.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


Extras: Filter Print Post Top
Jump to top Pages: 1


Similar ThreadsPosterViewsRepliesLast post
* Best Recipe for LC using spore needle to Knock up! Rylmonkey 850 7 09/16/05 02:58 PM
by HippieChick
* LC advice needed, ive got pure raw honey, what can i do with this? TheLegend 1,762 3 01/03/06 08:19 AM
by _OttO_
* E-Z long needles GnuBobo 854 8 06/28/11 10:41 AM
by S33D
* Sterile LC Transfers infinite420 1,137 8 09/10/06 12:37 PM
by
* small problem with a karo lc luckytriple6 1,438 11 08/26/05 09:34 PM
by Prisoner#1
* larger needle for core biopsy. will this work? Primate 2,351 12 10/31/06 12:31 PM
by Oatman2000
* can't break up LC
( 1 2 all )
etam 6,225 20 09/26/17 01:57 PM
by Roadkill
* Trouble getting LC into syringe. BLUESRX 972 7 12/13/06 09:37 AM
by Oatman2000

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
113 topic views. 31 members, 292 guests and 80 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.