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Fungus Amoungus
Stranger
Registered: 10/13/20
Posts: 2
Last seen: 3 years, 2 months
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Sterilization/Reuse of Multispore Syringe
#26994417 - 10/20/20 11:52 AM (3 years, 3 months ago) |
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Hello, I've begun my first PF Tek grow (cronicr's container method) by inoculating multiple plastic containers with my 2 MS syringes. There were still 3-4 CCs left in each syringe when I was done, but I believe I improperly stored these and perhaps introduced contaminants.
For some reason when finished, I decided to put the syringe cap back on, but then take both needle & cap off, and re-placed the original plastic cross on the syringe (I also did this outside of my SAB, silly me).
Did I inadvertently introduce more contaminants to the syringes (which contain inherently unclean spores already from what I've read) for when I decide to reuse these the next time? I did not retract the plunger at all, potentially introducing filthy air into the syringe, but I imagine the plastic cap area that connects to the needle will be contaminated.
If I've screwed up these syringes I suppose I could always try my hand at agar for the first time (don't have any materials for it, though) to get a clean sample of mycelium, otherwise if I'm overthinking this, I will continue to use these to inoculate BRF PF tek style containers until the syringes run out.
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fraki58
Mycology Learner


Registered: 11/20/19
Posts: 245
Last seen: 5 days, 10 hours
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Re: Sterilization/Reuse of Multispore Syringe [Re: Fungus Amoungus]
#26994454 - 10/20/20 12:11 PM (3 years, 3 months ago) |
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I don't know if you screwed them(more than they prolly were, cause as you know, they aren't clean to begin with), but, with what's left of them i highy recommend you start learning how to do agar work! This way you can clean up your spores and make sure the inocculus you are using is clean, or at least looks like it. I started doing agar as my first step in this hobby and i thank for it, once you get some spore plates and transfers under your belt you'll start to flow with it. Oh and also, Good luck with those cakes!
-------------------- If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best
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PNDub
Traveler

Registered: 05/19/10
Posts: 52
Last seen: 3 years, 1 month
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Re: Sterilization/Reuse of Multispore Syringe [Re: Fungus Amoungus]
#26994464 - 10/20/20 12:16 PM (3 years, 3 months ago) |
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When in doubt, put it on agar and transfer it out!! Haha but seriously I don’t think there is any way to know for sure if you’ve contaminated the syringes. If you didn’t suck any unclean air back in I would assume you can just wipe with iso and flame sterilize between jars but as you already noted, there is a risk involved in using MSS without growing out on agar (perhaps even more so now).
Another idea is to take a clone of any fruits you may get from your first attempt. Agar is cheap and once you’ve had some practice, pretty simple.
Edit: check this https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954
Edited by PNDub (10/20/20 12:24 PM)
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Fungus Amoungus
Stranger

Registered: 10/13/20
Posts: 2
Last seen: 3 years, 2 months
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Re: Sterilization/Reuse of Multispore Syringe [Re: PNDub]
#26994921 - 10/20/20 04:28 PM (3 years, 3 months ago) |
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Thanks for the replies.
PNDub I've actually already started reading some of that! I'll dive some more into it since it's a great resource for starting agar.
Based on the relatively good success of MSS for PF Tek stories I've read despite its inherently less than ideal media conditions, I'd prefer to stick with BRF syringe inoculation, barring that I pushed the chance of contamination over the edge with how I handled my syringes (but it sounds like I shouldn't be too worried).
I think I'm planning on doing a hybrid of standard SGFC and a mini monotub (from Whyte's Tek), based on how many of my containers end up colonizing 100%
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PNDub
Traveler

Registered: 05/19/10
Posts: 52
Last seen: 3 years, 1 month
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Re: Sterilization/Reuse of Multispore Syringe [Re: Fungus Amoungus]
#26994948 - 10/20/20 04:49 PM (3 years, 3 months ago) |
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PF Tek is super fun for me and IME you can get plenty of fruits. I did like 48 jars on my last attempt and got 3 flushes before a single cake contaminated. And I’m fucking clumsy and using a tiny ass still air box haha! Good luck! Cool name btw.
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