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Offlinepitriot
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Registered: 06/16/19
Posts: 455
Last seen: 3 months, 23 days
Swabs
    #26993773 - 10/19/20 10:33 PM (3 years, 3 months ago)

How do swabs work and how to use them, can I use them to inoculate grain or LC or just agar? Why do some strains come in swabs while others in MSS?


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Invisiblealaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,688
Loc: The 49th Dimension
Re: Swabs [Re: pitriot]
    #26993795 - 10/19/20 10:53 PM (3 years, 3 months ago)

Swabs are typically used to gather spores from varieties and species that don't deposit spores well (or at all).  They are used to introduce spores to agar (not LC or grains).  They come in boxes of long, sterilized medical swabs or you can make them by wrapping regular qtips in foil and putting in a jar to PC.  They're pretty darn simple: swab gills and store,  then swipe your agar plate to introduce spores.  :thumbup:


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Offlinepitriot
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Re: Swabs [Re: alaskappalachian]
    #26994102 - 10/20/20 08:07 AM (3 years, 3 months ago)

How many agar plates is one swab good for?


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OfflineCamera93
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Registered: 08/15/18
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Re: Swabs [Re: pitriot]
    #26994115 - 10/20/20 08:13 AM (3 years, 3 months ago)

how many plates could one swab swab if one swab could swab plates

it'd swab all the plates that one swab could swab if one swab could swab plates


pry a good many, but it seems unless they are fresh a lot of people turn to burying the swab in agar to get it germinate, or cut pieces of the swab off. This will dramatically reduce the number of plates one swab could swab


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineThe Mycologist
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Re: Swabs [Re: Camera93]
    #26994131 - 10/20/20 08:29 AM (3 years, 3 months ago)

Yea, only two or three plates if you rip the swab up.

I think that its the best way to insure germination.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Invisiblealaskappalachian
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Registered: 10/22/19
Posts: 1,688
Loc: The 49th Dimension
Re: Swabs [Re: The Mycologist]
    #26994213 - 10/20/20 09:30 AM (3 years, 3 months ago)

I go for two plates when I swab and call it good.  Don't see a reason to do more.  Just a waste.  Unless you're using really old spores and hard AF agar,  your spores are going to germ just fine and you can use your plates for xfers instead.  Only time I've done more in recent memory was the other night actually when I swiped an angulospora swab and I buried the tip on the third.  Swiping more plates with spores that won't germinate wouldn't likely help anyway because swiping more plates with shit spores will just leave you with more dud dishes as opposed to less dud dishes.  Can't even recall last time spores wouldn't germinate.  Two and done is my rule personally and that's just in case of a heavily contaminated swab giving me trouble with bacteria or mold that expresses early,  complicating a clean first transfer; and even those circumstances are rare IME.


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