Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Making spore syringes from swabs (I’m using an oven bag**optional) * 8
    #26993868 - 10/20/20 12:45 AM (3 years, 3 months ago)

**I’ll be updating the OP as things progress

INTRODUCTION


So I’ve done this a couple times successfully now (A 3rd time (PE) is in the qt colonizing stage and the 4th time (Texas orange cock) was done 2 days ago and awaiting germination).. I use an oven bag instead of a flow hood or SAB because you can pc an oven bag and I’m pretty anal about my sterile technique, but the process would be the same for a SAB or flowhood.  **the pics of the oven bag construction and procedure will be at the end of this post

The swabs I use are the presterilized kind.. I think I paid $17 for a 100 of them



PROCEDURE AND EXECUTION


One thing I’ve found is that you want your swabs to sit and dry out (in the little package they come in—after you take the spores) for at least a week.. I believe this helps cut back on bacteria because it dries the bacteria out, killing it (non-Endospore producing bacteria anyway).. any swabs that I used right after swabbing didn’t fare as well as ones that sat.

The 1st time I did it, I made a melmak syringe from 1 swab by taking a jelly jar of water (Used about 20mls of water—just enough for the fiber tip to be submerged.. worked ok but I wished I had used more like 30mls) and mashing the swab tip against the jar wall/bottom while submerged.  With the syringe, I made 6, 1/2 pint pf cakes... I was expecting to lose most of the jars due to always hearing that if you make syringes from swabs, they’ll contaminate.  I figured at least one would make it long enough that I could take a leading edge if myc from the colonizing cake (birth early) and clone it. 

4 of the 6 pf cakes germinated and grew out enough that I took a small piece of leading edge myc from a pf cake (birthed it early and not fully colonized) and cloned it to jelly jars of grain for a GLC.  Here’s what those look like.. (I used my Oven bag cloning method)



This was right at the end of my growing season so I only grew out one tub and it reverted (or whatever weirdness melmak was doing around that time—there were a few members that reported non-melmak phenos from known melmak swabs) so I didn’t get that melmak pheno I was looking for but it did tell me that swabs can be made into spore syringes successfully which is a win in my book.  Here’s how that tub turned out (did non-melmak things)



The second time I did it (10/04/20) I made a PE/AA+ swab (swabbed both PE and AA+ to the same swab—I had a mature tub of both varieties and went from one cap to the other.  This is in hopes of creating a cross between the two varieties) into 1 syringe.  This time, when I made the syringe I used two swabs instead of 1... that way I could rub the 2 swabs tips together while under water, further incorporating the varieties’ spores to get a cross and so I didn’t have to mash the tip against the jar wall.

The other thing that I did differently (what I think is the most important part to pull this off) the 2nd time and on, was to do a prewash on the swabs before putting the tips into the water I was going to use for the syringe (I may or may not have gotten the idea from reading Josex’s poke method—can’t remember if I saw his thread before or after I attempted it lol.. either way, josex is a genius and I probably got the idea from him)... so in the oven bag (or SAB or flow hood—which ever you use) I had two jelly jars of sterilized water instead of just the one...

The first one I just dipped the swab tips into and lightly rubbed the tip together to get off any contamination that might have landed on the swab and then went to the other jelly jar that had 30mls of sterilized water and vigorously rubbed the tips together to try and knock the spores off into the water... the second jar is the jar that I pulled from to make my syringes.  (for the 3rd time, I put a little piece of rubber that has tiny nubs on it on the bottom of the second jar of water so that I could rake the swab tips across it to knock the spores off into the water easier.. it worked well)



So far this has proven to be very effective.. with the syringes, I made 4 pf cakes and inoculated a jelly jar of grain so that I could make a GLC to knock up a couple qts to do a test run and see if I get any crosses.  I didn’t expect too much but all four of cakes finished colonizing and they looked great while doing so.. they were super fast and looked really clean and healthy.  They colonized in 14-17 days



With the cakes, I birthed 3 of them into a makeshift FC and left one for experimenting with some invitro ideas



All the cakes fruited (what I believe to be crosses if PE and AA+ at every inoc point) (except the jar one.. still waiting to see what that does fruiting wise)

.  


The 3 cakes dried down to 16.1 grams

I actually fruited that last cake and it put out a monster pea+ fruit that I cloned

.


The GLC grains colonized great too and I used them to knock up 2 qts which also grew just fine and were made into a mini mono which fruited great (cased half of it to hedge my bets lol)

.


I actually put the grains I used for GLC to CV after I let them recolonize after stealing a lot of their myc.. they flushed out already.. dried down to 2.5gm.. the perfect tester dose for me.  Update: I tested out 2 grams of pea+ tea and they’re on par with PE.. maybe not as potent as straight PEs but definitely better than regular cubes.. if PE is a 10, these are a 8.6.. i’d put regular cubes at a 5-6

. . .


The 3rd time is still in colonizing stage, but I made a PE swab into SS and went straight to rye jelly jars.. I made three of them and only put about 1-2 drops of SS to each.  The idea with that being that it reduces the amount of competing sub strains of cubes but also, if there is a nasty floating around in the SS, you reduce the chance that it ends up in all 3 of the jelly jars by reducing the amount of SS each jelly jar gets.  All 3 jars fully colonized without issue... one was used to knock up 4 qts.. they all looked like this 2 days after a shake



Currently 5 days after shake (don’t mind the condensation.. it was pretty chilly in the room this morning—it goes away by afternoon)



Probably give them another day before I spawn them. 

So.. so far, I’m either having extremely good luck or making SS from a swab isn’t as risky as everyone thinks... especially if sterile technique is taken seriously when making the swab and making the SS.  If you think about it, it’s more sterile than making a traditional print (cap sitting on foil—Traditional spore prints are exposed to open air for hours).. the swab comes commercially sterilized and if you do it right, the swab is only exposed to open air for about 30 seconds or even less.. that coupled with the prewash, makes this a viable way to store spores and make them into SS, imo/e. Obviously, swab to agar is a safer way to go about getting myc rolling and I’m not here to sway anyone away from going that route, but as long as you’re smart about it with sterility and go into it thinking you might lose some (make multiple substrates and use as little SS as needed), swab to SS is definitely possible and don’t be afraid to give it a whirl... I’ve found it to be just as reliable as making a syringe from print, if not more so.

And I’m not saying that because my subs didn’t mold out, they’re 100% clean.. I’m positive there’s at least some amount of bacteria just like when making a traditional print into SS.. but for my uses, I’m only trying to get to a first flush so that I can clone.. I don’t need it to go past that, so having a slightly bacterial culture because I made swabs into SS, isn’t a problem for me.  Again, not trying to sway anyone from using agar.. it comes down to me not trusting SAB’s.. I had a horrible experience many years ago where I lost like 12 monos when I was doing g2g in a SAB.. it was right before they were ready to pin too.. it was the WORST.. that’s why I developed the oven bag methods, GLC and my lid designs and since then, it’s been smooth sailing.. I lose very few jars and even less substrates.

MY OVEN BAG CONSTRUCTION AND PROCEDURE.


Let’s get started.. first thing I do is vacuum seal the swab(s).. I ISO the outside of the swab package and the inside of the vacuum pouch and vacuum seal it up... Then I carefully bend the pouch to break the swab sticks about 3” above the swab.. this makes it easier to handle the swabs when I’m dipping them in the water.. better leverage




Then set it aside.  On to making the bag..



Now, a lot of you probably know I love oven bags.. fracking looooOoOoove them.. they’re so versatile.  They can be pc’d.. attachments can be added to them easily.. they’re cheap.. and they’re malleable so I can work with the items placed inside easily with my hands from outside.  I also developed a syringe barrel poly-filled air filter that I hook up to a fish tank bubbler so I can inflate the bag so it’s easier to work with the items inside the bag

The idea is a really simple one... all the items that I need to do either a cloning or spore print to syringe or swab to syringe are secured inside the bag and then pc’d.. once it’s cooled, the swab or print or clone material is transferred to the inside of the bag quickly and carefully (via H2o2 with clone material—and I’m actually working on a method where H2o2 is used to transfer the stem into the bag and once it’s inside the bag, it’s torn open and tissue is taken, so the H2o2 never touches the actual cloned tissue making it 100% clean) through a small opening that was left when the bag was constructed and the bag is sealed back up.. then the process of making the SS is done from outside the bag while the items are secured inside. 

I’m not delusional and don’t think everyone is going to run and make this thing but maybe it’ll give some ppl ideas they didn’t have before... this is long and tedious (trust me I’m using a phone lol) but really it takes like 5 mins to set up a bag once you have all the parts and everything but the bag and zip ties are reusable

This is actually a combination of My oven bag clone tek and the little known IDCAB :lol:  unlike the IDCAB, I’ve actually used this successfully, as I’ve said, 2 times and plan on using it many more.

ITEMS YOU'LL NEED


-  turkey sized oven bags



- Two 2”, 3/4” Dia, pc-able tubing stuffed tight with poly fil and an empty syringe barrel also stuffed tight with poly fil.



-  4” small zip ties



-  tape (I use blue painters tape)

-  empty syringe(s) with plunger (Sometimes I use 1 but you’ll get more SS with 2, obviously)

-  small metal scissors

-  two jelly jars with lids

-  small plate

-  tyvek

BAG ASSEMBLY

1.  Lay your bag out flat and cut just enough off of the sealed corners that the tube of poly will fit through


2.  Attach the tubes with 2 zip ties so that the zip tie heads are opposite each other (this is so if there’s a gap at the zip tie head, having two of them closes each other’s gap—all attachments to bags are done this way)

Do that on both sides


I do this so that steam can enter the bag easily when it’s sealed and the bag doesn’t explode in the pc

3. Cut a small hole and Take your syringe barrel stuffed with poly and attach it to the bag in the middle between the tubing vents... make sure you attach it so that that needle port is on the outside (this is where I plug the hosing from the fish tank bubbler to inflate the bag)


4.  Take some tyvek and make a little pouch that the 2 empty syringes and scissors go into.. this is just to keep those items from poking the bag and keeps them nice and together
.

5.  Take your 2 jelly jars (4oz) and fill them with 30ml of water each... in one of the jars, I use a piece of rubber with nubs on it in one of the jars so that I can rake the swab tips across it to dislodge the spores from the swab.. this is the jar that I’m going to pull the SS from.  The other jar is for the prewash.


6.  Screw the lids on loosely and put them on the plate with your tyvek pouch on top of the jars (or under one of them like in the pic) and place the plate into the oven bag and push it towards the back


7.  Now I seal most of the open bag end by folding the edge over about a 1/2 inch and tape it closed so that all the tape is touching and it’s flat.. I’m making it air tight.  But make sure you leave just enough open so that you can slide the vacuum sealed pouch of swab(s) into the bag


8.  Take that little section that is untapped and roll it up and fold it over (do that a couple times) and zip tie it somewhat loosely.. this just secured the open part so it doesn’t get exposed to air before we’re ready to use it after pc’ing.  Before you do that make sure you push as much air out of the bag as possible


9.  Now I just ball it up as small as I can and make a foul ball (big piece on the bottom that it sits in and a big piece over that) similar to this


10. Pc this for 45 mins (You can probably do less if you want but that’s what I do) if you’re just doing swabs or prints @ 15psi and 1.5 hours if you’re doing a clone to grain and need to sterilize the Jelly jar of grain in the bag.

Now the fun part..

SWAB TO SS (spore solution)


**some pictures might look different because they were taken during a different swab to SS session and since it was only me, I wasn’t able to get some pics of the whole process.. just gonna have to use your imagination at times

1.  Once the pc is cool, take your vacuum sealed swabs and Iso the whole thing.. then take a piece of paper towel and soak it with H2o2.. do the same with another piece and sandwich the vacuum pouch between them.. do this in the area where the transfer into the bag is going to take place.  The idea with this is that it will keep the outside of the pouch clean while you’re setting up the rest of the bag for the transfer


2.  Take the ball out of the Pc and unball it.. lay the bag out so the part you left opening for the transfer is right by your H2o2 pouch sandwich.

3.  Cut the zip tie closing off the transfer opening and unfold and unroll it, keeping the bag as flat as possible... were trying to keep out as much air from inside the bag as possible.

4. Very quickly, slide the pouch into the bag.. again, keeping the opening as flat as possible.

5. After the pouch is in the bag, reroll and fold the opening back up and zip tie it closed.  Here’s the bag after the pouch is inside and the zip tie is on


6. Once the pouch is sealed back up, you need to attach the fish tank bubbler hose to the syringe needle port and turn it on to inflate the bag.. takes about 10 minutes to get the bag to a manageable size
. . .

This is so the bag doesn’t collapse on your work. 

7.  Once the bag is inflated enough, unplug the bubbler.

I don’t have a lot of pics of the next processes because it was only me and I only have two hands lol, but maybe I’ll update later with pics if I have a helper

8.  Take the scissors out of the tyvek pouch (carefully... don’t want to cut the bag) and put your fingers through it from outside the bag.. takes a bit of practice because you need to have slack In the bag in between the scissor holes so when you open the scissors, the bag doesn’t stop you from doing so.


9.  Carefully cut the vacuum sealed pouch (cut the swab pouch at the same time) off around the swab sticks a little above where the break is.. so about right where I’ve drawn this line


10.  Once you’ve cut around the sticks, you can pull that short piece off that has the swab tips in it and the stick ends will be sticking (no pun intended) out.  Put the long pice aside, it’s no longer needed

11.  Now unscrew and remove the lids on your jelly jars.

12.  Pull the swabs out of the cut pouch and take one in each hand (if you have two.. and make sure you hold onto the sticks tightly.. don’t want them to poke through the bag) and dip them in the prewash jar a few times to remove any nasties that might have landed on the surface of the swab.. I rub the swabs together lightly if there’s two.. if only using one, touch the swab tip to the side of the jar wall a couple times

13.  Now take the swab tips to the second jar with the rubber pad in it and dip the swabs in the water and vigorously rub the tips together.. after that, rake the tips Across the surface of the rubber pad that has nubs on it... this helps dislodge the spores from the swab tip.. do this until the swab looks like most/all of the spores have been released into the water

14. Put the swabs aside, we’re done with them.  Take your syringe out of the tyvek pouch and carefully (don’t want the needle to poke the bag—best to not let it touch anything but the water/jar) suck up your SS from the second jar.. like the scissors, you need to take a lot of bag slack between the hand holding the syringe barrel and the hand pulling the plunger otherwise the bag is going to stop you from pulling the plunger all the way up. 

Once the syringe(s) are full, you’re done!  Rip the bag open and retrieve the syringe(s).  Make sure you cut off the reusable pieces on your bag (tubing, syringe barrel) before you throw the bag away.

You can also do traditional prints to SS with this contraption.. all the set up is the same (clean and vacuum seal a foil print) as with swabs, but instead of dipping swabs, you just use one jar of water and cut the foil print out of the vacuum pouch.. then open the print and scrape the spores into the jar of water, either with the syringe needle or with another metal tool/scraper and suck the SS up into the syringe.  Here’s me holding the open print.. you can see the little scraper I pc’d inside the bag (went into the tyvek pouch)


I successfully made AA+ SS from a print this way.. first flush:


Second flush


UPDATE 5/8/21:  I’ve used this a lot since I made this thread.. here’s all the grows that I went from swab —> spore solution..
PE/crs:


PE/RW


PE/pesa


PE/apeu


Even if I go straight to small grain jars and the grains didn’t look as healthy as I wanted, I just top fruit the jars and get clone material or a new swab


Hell.. I’ve even taken swabs that were both broken at the swab tips from shipping and directly injected sterile water into the swab pack, massaged around the water to dislodge spores and sucked the water back out (it has to be a really clean swab/swab pack—thanks, filthyknees) and got these grows from them (apeu):


I know that’s a lot and if you read the whole thing.. thanks :lol:  minus the pc time, it’s all really fast.. 10 minutes to set up the bag and get it ready for the pc and 15 minutes to do the actual transfer and spore collection and I feel much better about doing it than using a SAB, but that’s just me

Faht



Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery

Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27028655 - 11/08/20 01:47 PM (3 years, 2 months ago)

This thread was moved from the user's journal.

Reason:
Go easy on me.. I know it’s quite the contraption :lol:  but hey.. so far it’s working for me :wink:

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27039018 - 11/14/20 03:32 PM (3 years, 2 months ago)

PEA+ F2 syringes made



I’ll be making 4 pf cakes in a couple days

Edit: One cool side effect from rubbing the two swabs together is that when I hold the syringes to the light and look at them, there’s a ton of tiny tiny swab tip particles floating around.. those particles undoubtedly have spores stuck to them.. so they act as a sort of built-in suspension mechanism and I don’t have to worry about free floating spore sticking to the inside of the syringe barrel.  Pretty neat

Faht


Edited by fahtster (11/14/20 03:45 PM)


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible... Flag
Last seen: 1 hour, 34 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27039034 - 11/14/20 03:37 PM (3 years, 2 months ago)

Really cool Tek! Thanks for moving from your Journal where it belongs now :yesnod:

I have some cottonswabs that I need to use soon and this Tek is definitely helpful to the process :thumbup:


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos] * 1
    #27039406 - 11/14/20 07:27 PM (3 years, 2 months ago)

Thanks, pal!


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible... Flag
Last seen: 1 hour, 34 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27039557 - 11/14/20 08:48 PM (3 years, 2 months ago)

:awesome:

I have three questions. First, I noticed there is blue painters tape around some of the lids. Is this to prevent rusting? Or perhaps to make opening the jars easier?

And your "pre-wash" technique. Have u tried that on spore prints to "wash" them using a swab?


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos]
    #27039619 - 11/14/20 09:55 PM (3 years, 2 months ago)

The lids are just the ones I use for making GLC.. https://www.shroomery.org/forums/showflat.php/Cat/0/Number/6886717/an/0/page/0. I switched to painters tape instead of duct tape.  I just had them on there so the jar had a lid in case it spilled.

I haven’t tried that with a print, but I don’t see why it wouldn’t help.. if I’m understanding you correctly lol had some tea earlier

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27050790 - 11/21/20 05:23 PM (3 years, 2 months ago)

I keep getting nice little side effects from going from swab to SS.. so I made the f2 syringes on the 14th and didn’t get around to making the cakes for them until a couple days ago.. because of all the tiny bits of swab material with spores attached that comes off the tips due to the rubbing and ends up in the syringe, the spores germinated while the syringe sat.. the “tiny” bits turned into large bits when I went to inoculate the cakes.. the swab material is cotton and I used tap water so this makes sense. I already have myc growing after two days at all inoc points



While this exciting, it also means that any contamination could have also fed off the cotton while it sat so I’m hoping the prewash did it’s job and that there wasn’t any mold spores mixed in with the mush spores that I grabbed with the swab. The prewash isn’t going to do much for those. So fingers crossed!

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084923 - 12/12/20 01:10 AM (3 years, 1 month ago)

A little update.. I came up with a new plan to print some pea+ f1 fruits I have just starting to fruit.. gonna print cleanly to the bottom of a jar (I’ll post full procedure later on) and go straight to grain instead of doing cakes to help speed stabilization along.. so I scrapped 3 of the 4 F2 cakes because I needed the jars to do a PE/prfs cross attempt.  I kept the furthest along of the 4 cakes to fruit to get a f3 fruit and it’s just about fully colonized (I think there might be a few knots on the top actually)



I’ve also got a jelly jar of PE/pfrs double swabbed that I did exactly like the pea+ described above that is almost fully colonized and ready to be glc for a couple qts



I’ll keep the updates coming as this moves ahead

Faht


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084925 - 12/12/20 01:21 AM (3 years, 1 month ago)

:camping:

Here to witness whatever cool madness comes out of this. That clean printing procedure piqued my curiosity, too. :popcorn:


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Josex]
    #27084952 - 12/12/20 01:59 AM (3 years, 1 month ago)

That's awesome fahtster! I got to try this technique. Seems really helpful for PE varietys.

Also I did send out some PA+ prints to a few people, mine was a cross between PEU and AA+, but it lost its PEU pheno in the process. I'm working on a new version of it from F3 and will post when I have some results.


Those PEAA+ look really groovy.


Edited by Sockadin (12/12/20 02:00 AM)


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Josex]
    #27084954 - 12/12/20 02:04 AM (3 years, 1 month ago)

Quote:

Josex said:
:camping:

Here to witness whatever cool madness comes out of this. That clean printing procedure piqued my curiosity, too. :popcorn:




Pretty big fan if your PE thread from a couple months ago too, man.  I like the way you think.  I’m gonna do one of your pokes on the inner stem tissue of a fruit on my next PE bin coming up.. figure I can do it fast enough that I can get away with an open air.. figure I can flame the needle, then tear the fruit and have the lc jar real close that I can do the poke and inoculation within a few seconds.. I’m hoping to get enough flesh that going through the SHIP will be ok.. I figure fruit tissue will be denser than agar myc and itll make it through and I can get enough pressure on the plunger with the water to shoot it into the grain water.. I’m gonna make an attempt anyway lol

I forgot I posted a concept of the clean printing here.. thought I only did it an ‘topia.. easy copy paste lol

“Gonna line the inside of a jar with foil (Just the sides and top rim so that the bottom Is exposed)  then take a longish skinny screw and screw it into the stem butt.. then put the cap with screw inside the jar so that the screw head sits on the bottom and the cap rests against the foil.  Then after the spores drop, just flip the jar upside down and hold the foil lip and pull the jar off leaving the cap and foil behind

After thinking about it a bit, I might just zig zag a 1/2” flat (but sitting vertical so the spores can fall to the bottom of the jar) piece of foil on the bottom of the jar so the cap can sit on that instead of using a screw.. gonna play around with both before I attempt the real deal in the oven bag

.

I’m going to put a nut or something similar that’s magnetic and put one of my adjustable airflow lids with SHIP.. I’ll use a magnet to move the nut around from outside the jar to scrape the spores into the water



I’m going to pc the foiled jar with lid and the screw and nut inside an oven bag.. just slip the cap in after the bag cools from the Pc through a little slit in the bag.  Should work like gang busters and give me clean spores that I can go straight to grain with.”

I’ll update with pics of the process when I actually do it here in a few days... and I’m aware of how facking crazy all this oven bag shit is :lol: but I’ve made so damn many of these things over the years that I’m extremely good at constructing them and using them.. I actually updated my oven bag clone method to exclude h2o2.. now I just use sterile water from a syringe to fill the quarantine section and have 2 jelly jars of water I pc inside the bag that I use to rinse the clone material before I put it in the grain jar.. I did that for the first time about a week ago so the jury is still out but the growth looked good before I shook the jars, but I feel really good about the method... I really wanted to get rid of the H2o2 soaking of clone material and I think this going to work great and it was super easy.. the peace of mind alone is priceless.. nothing worse than worrying if your culture survived being soaked in a caustic liquid :lol:



Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Sockadin]
    #27084961 - 12/12/20 02:13 AM (3 years, 1 month ago)

Quote:

Sockadin said:
That's awesome fahtster! I got to try this technique. Seems really helpful for PE varietys.

Also I did send out some PA+ prints to a few people, mine was a cross between PEU and AA+, but it lost its PEU pheno in the process. I'm working on a new version of it from F3 and will post when I have some results.


Those PEAA+ look really groovy.




Nice man!  I sent/sending a few out to tribe members to help stabilize it.. I trust em not to send it out too early and pick only the desirable traits.  They’re out there and I’ve got a nice stock pile of f1 genetics that eventually it’ll be available to all.  I’m putting this clone to grain qts tomorrow/today when I wake up via glc

. .

Culture looks nice and clean



For that one, I transferred the entire intact stem into the oven bag via h2o2 and split the stem and did the transfer while inside the bag.. that was what I thought up before the sterile water idea but it was still using H2o2 and didn’t like it even though the clone tissue never touched the H2o2

Faht


Edited by fahtster (12/12/20 02:21 AM)


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084964 - 12/12/20 02:21 AM (3 years, 1 month ago)

For fun here is the PA+ that was a cross between PEU and AA+.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Sockadin]
    #27084969 - 12/12/20 02:29 AM (3 years, 1 month ago)

Yep.. definitely looks like aa+ and peu had a baby :lol:  did the pin times improve with the aa genes in there?  My peu took even longer than regular PE to pin... and then the flush sucked lol



Could’ve had a shitty culture and/or bacterial etc but I did not like peu at all.. but man, if this keeps working, I’m going to put PE genetics in anything I can get my hands on.. wonder what a PE/peu cross back would “fruit”

Faht


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084975 - 12/12/20 02:50 AM (3 years, 1 month ago)

Yeah it wasn't bad if I remember correctly. Was back in June and I haven't had a chance to work with them since.

PE/PEU would be interesting. 6week wait for pins lol.


I want to do a PEU/Z-strain cross this winter. I love how dense and heavy the stems are on the Z, and the Pheno of the PEU.

We should start a Penis Crossing thread and see what everyone comes up with. We can call it the sword fighting thread.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Sockadin]
    #27084978 - 12/12/20 02:55 AM (3 years, 1 month ago)

:lol: I’m down


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27096883 - 12/18/20 11:25 PM (3 years, 1 month ago)

Quote:

“Gonna line the inside of a jar with foil (Just the sides and top rim so that the bottom Is exposed)  then take a longish skinny screw and screw it into the stem butt.. then put the cap with screw inside the jar so that the screw head sits on the bottom and the cap rests against the foil.  Then after the spores drop, just flip the jar upside down and hold the foil lip and pull the jar off leaving the cap and foil behind

After thinking about it a bit, I might just zig zag a 1/2” flat (but sitting vertical so the spores can fall to the bottom of the jar) piece of foil on the bottom of the jar so the cap can sit on that instead of using a screw.. gonna play around with both before I attempt the real deal in the oven bag





Actually did this.. went with the screw screwed into the stem butt.. worked out nicely



Gonna dry it out for a few days and try going straight to grain

Faht


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible... Flag
Last seen: 1 hour, 34 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27096899 - 12/18/20 11:39 PM (3 years, 1 month ago)

Thats awesome. Im guessing like a wood carpenter screw to elevate it off the glass?


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos] * 1
    #27096902 - 12/18/20 11:44 PM (3 years, 1 month ago)

Yessir

Lined a jar with foil like so


Then put that with lid loosely on the jar on a plate in an oven bag with most of the opening taped up except for enough for the cap to fit through (these are pics from when I made swabs into SS but the set up is the same)
.

Put the screw and other tiny screw (that I put in the jar with the print so I can send magnetically move it around to scrape the spores into the water) in a foil pouch and put it in the bag with the plate and jar and pc’d the bag

Then when it was cool, slid the cap through the little opening and sealed the bag back up and then, from outside the bag with my hands, screwed the screw into the cap stem butt and put it in the jar and loosely put the lid on and waited for the spores to fall... then flipped the jar upside down and pulled the jar off leaving the foil and cap out.. then put the tiny screw in the jar and screwed the lid on.. then cut the bag open and removed the jar. 

Faht


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible... Flag
Last seen: 1 hour, 34 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27096909 - 12/18/20 11:49 PM (3 years, 1 month ago)

Very cool man :awesome::thumbup:

I gotta try that next round :yesnod:


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos]
    #27140119 - 01/10/21 04:11 PM (3 years, 18 days ago)

So I’ve got the PE/pfrs RADS (rub and double swab) in fruiting chambers.  16qt tub, 2 qts of rye spawn to CV.. The qts were inoculated with GLC made with the RADS SS.  It’s been 4 days



The last time I did this^^^ with pea+, I got an entire bin of cross fruits, so here’s to hoping :cheers:


Here’s the coir cakes I made with the RADS SS.. it was the first time attempting coir cakes and I made them too wet.. I ended up having to cut off some uncolonized areas at the bottoms of the cakes so hopefully I went far enough and they don’t tam out.. I’m hopeful though.  They’ve also been in the FC for 4 days



So hopefully in the next couple weeks I’ll know if I got my coveted red spore PE variety. :grin:

Today I put my pea+ f1/pfrs RADS brf cakes to a FC. I figured the best possible thing that could happen trying to cross the f1 pea+ with pfrs is, I could end up with a red spore pea+, a red spore AA+, and a red spore PE and the worst thing that could happen is I end up with an F2 pea+.. seems like a win-win to me, so I said fack it, let’s try lol



Those cakes colonized great and look awesome.. they reminded me of the first pea+ RADS SS cakes I made and got crosses at almost every inoc point, so I’m pretty excited about this grow.  The qts I made with the glc of that RADS SS we’re shook yesterday and they looked great pre-shake



When I made the glc grain jars for those^^^ qts, I made two 4oz rye jars... one I used to inoculate those qts and the other I spawned to CV in a little glad container and it’s probably a fewish days away from knots atm..


That’s why I like those little testers.. they give me a glimpse of what’s to be in a couple weeks

I Spawned these pea+ F2 qts to CV in a 16qt today too.. these were the qts I went spore print straight to grain that I used the in-jar printing method from a couple posts ago



Lots of fun and exciting possibilities coming up in the next couple weeks!  Really need the mush gods to shine on me :lol:

Faht


Extras: Filter Print Post Top
InvisibleAll41
Self


Registered: 06/01/20
Posts: 85
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27177326 - 01/30/21 02:30 AM (2 years, 11 months ago)

Why do you like the print on the bottom of the jar if your making swabs anyway.


Edited by All41 (01/30/21 02:34 AM)


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: All41] * 1
    #27177332 - 01/30/21 02:44 AM (2 years, 11 months ago)

So I can come back to the print and grab spores from that gen later and it’s cleaner than a swab.  Swabs are easy to make.. takes like two minutes.  The swabs are mainly just back up.  The printing in a jar is whole process that I use an oven bag that I pc and is kind of a PITA compared to swabbing but I trust it more to go straight to grain with.

Kind of forgot about this thread lol.. I have results from all those tubs..

the pea+ F2


The PE/pfrs cakes


Got a bunch of crosses on the cakes.  Purple spore dropping/heavy PE pheno fruits.  The purple dropping spores is a dead giveaway for a cross


This guy I printed (redspores) and I believe is a cross based on color/cap shape/stem bumps.  They’re already germinating on rye


Pe/pfrs 16qt bin.. I believe there’s lots of crosses going on in here.. no reason to think differently based on past experience with the cakes above and how it worked with pea+ and the pea+ f1/pfrs bin.. the PE is showing through on a lot of fruits.  I cloned a couple


Pea+ f1/pfrs produced normal looking fruits that were super fast (14 days spawn to harvest) and didn’t look like PE, AA+ or pfrs.  They produced purple spores but the cap shape isn’t like any of those 3... totally not what I was expecting lol. I took swabs of these but I’m not going to pursue these further for now.. this was just for fun to see what would happen if I crossed an f1 fruit with something


For reference, this is what pea+ looks like fruiting (we all know what PE and AA+ look like)


The next cross I’m going to be working on is PE/crs.  Making some qts tomorrow.  I will be moving forward with pea+ and the PE/pfrs.  Here’s 1 of the 2 pea+ F2 fruit prints I’m going forward with


Faht


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27307989 - 05/14/21 07:46 AM (2 years, 8 months ago)

Sick deal man. I was thinking of doing something similar, especially  after reading josex double dip idea.
Thank you!


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27308085 - 05/14/21 09:09 AM (2 years, 8 months ago)

I’ve got to update this thread lol. I’ve done this successfully a bunch more times

Faht


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27308088 - 05/14/21 09:11 AM (2 years, 8 months ago)

I'm going to be honest man, the OP is pretty intimidating for the tek, allot of info there

But thank you so much <3


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27308119 - 05/14/21 09:31 AM (2 years, 8 months ago)

You can get rid of all the intimidating info and keep it really simple.. take two swabs... sterilize two jars of water... dip the swabs in the first one a few times... go to the second jar... put the swabs in the water and rub them together where the spores are until they’re free of spores... suck up the water with the spores in it.  Done.

If you do it in front of a hood, that’s the best.  You can do it in a SAB or you can get crazy like me and do it in an oven bag.  The results are the same.  :thumbup:

Faht


Extras: Filter Print Post Top
OfflinekingboomerDiscord
smurfhouse archetect
Male User Gallery


Registered: 06/15/04
Posts: 739
Loc: Rocky Mountain High!!!! Flag
Last seen: 1 day, 13 hours
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27371204 - 07/02/21 02:46 AM (2 years, 6 months ago)

How would one go about this with 2 caps?  Smaller the better im thinking?  Sorry if this is OT...


-Kingboomer


--------------------
Hole in the sky/
take me to heaven..
window in time/
through it I'll fly...

-Black Sabbath


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27371378 - 07/02/21 08:15 AM (2 years, 6 months ago)

What do you mean exactly?  You can use any size cap to swab.. I prefer bigger ones since the gills tend to be spaced farther apart :thumbup:  or maybe you’re referring to something else?

Quote:

QM33 said:
I'm going to be honest man, the OP is pretty intimidating for the tek, allot of info there

But thank you so much <3




Oh shit.. didn’t realize we had conversed in this thread and here I am linking you to it :facepalm:  I’m getting old, man.. can’t even remember a month and a half ago :lol:  my B

Faht


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27371391 - 07/02/21 08:28 AM (2 years, 6 months ago)

Ha lul. No worries bro, I'm sure you have alot of important shit to keep in your brain.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27458443 - 09/06/21 04:05 PM (2 years, 4 months ago)

Making a PE/crs F2 swab into spore solution and thought I’d document the process a lil better.  These f1’s didn’t drop spores so I had to swab


Here’s just an update to how I’m getting this shit done.. the H2o2 “portal” is newer since I made this thread

The set up


What’s pc’ed in the oven bag:
-4oz rye jar (this was already pc’d once for 2 hours so I got not have to pc it inside the oven bag for 45 minutes to sterilize everything else).  I could just inoculate this jar outside the bag but I’d rather not inoculate through an iso wiped SHIP
-2 jars of water.. one is to rinse the swabs and the other has 17ml of water (just enough to submerge the swab tips) and that’s the jar I rub the spores off in and suck up via syringe
-empty syringe
-SS scissors
-A plate to hold everything

I tape up the bag opening all but about ~3” on one side and then roll and fold that opening and then zip tie it and pc for 45 minutes.  I also make 2 CV/brf cakes that I pc along with the bag

After pc’ing, I blow the bag up a little via the syringe barrel poly filter and fish tank bubbler (takes about 5 minutes.. the filter is very restrictive)  Then I use a modified glad container filled with h2o2 as a way to get the swabs into the bag without exposing it to room air… the swabs are vacuum sealed (swabs and inside of vac pouch is iso’ed prior to sealing.  I break the swab sticks about half way up so I can bend swab pack to get it through the bag hole easily and so they’re more manageable inside the bag.

I cut the zip tie and unroll and unfold while it’s submerged in the H2o2.. the bag opening is kind of stuck together from pc’ing which is nice so there’s not a lot of chance for air to get in until I physically pull it apart when it’s submerged.

Then I stick one end of the swab pack into the bag opening under the H2o2 and pull it into the inside of the bag… then I zip tie the bag opening back up while it’s still submerged


Then I blow the bag up more so there’s plenty of room to work in

I use the scissors (very carefully as to not cut the oven bag) to cut open the swab pack just below the break in the sticks and pull the back off.  While I didn’t let any air inside the bag, I still don’t want to touch the swab tips to anything except the jars of water, so I leave them inside the vac pack until I screw the lid off the first rinse water jar. 


I remove the swab from the vac pack and dip them into the first jar of water.. in this jar, I rub together all the parts of the swab tips that don’t have spores on it and also gently rub once or twice where there are spores… the idea with this first jar is to rinse off any possible contams that may have landed on the swabs when swabbing the gills.  Then I set the swabs in that jar while I take the lid off the second spore water jar.


Then I vigorously rub the swabs together where the spores are until they’ve been dislodged into the 17ml of water… suck up 1ml (2ml if I’m inoculating 2 grain jars) into the syringe and inoculate the grain jar… then suck up 10ml into the syringe.  Cut the bag open and remove the syringe and grain jar.

Here’s a comparison of how well rubbing the swabs together dislodges the spores into the water.. this is after I have cut the bag open.. before:

After:


Then I cut off the reusable pieces from the bag and discard the rest.. then inoculate the cv/brf cakes


Here’s how the pea+ f5 grain jars (I shook the grain jars yesterday so they should be done in the next couple days) and cakes are doing from the swab to SS I did last week.. notice how well the rubbing declumped the spores from the swab tips


I’m actually swabbing the prints ppl send me so I can make these because it works so well

Faht


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 26 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27458478 - 09/06/21 04:44 PM (2 years, 4 months ago)

Just gonna add this to my threads for later

:thumbup:


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: A.k.a]
    #27511988 - 10/21/21 02:40 AM (2 years, 3 months ago)

Thought I’d add to this what my next plan of attack is because I think it’s a good one and gonna make things real easy.. pretty simple.. instead of going through the trouble of making a syringe after I rub the swabs together in the second jar of water, I’m gonna just stick the swab tips into the grain jar and twist to get the spores into the grain. 

It worked when I basically streaked the swabs across the top of the grains when I made the Gandalf jars.. I’ve been making pf cakes along with the glc grain jars as back up but they’ve been unnecessary so might as well just cut out the extra step of making a syringe and just dip the swab tips directly into the 4oz grain jar (after rubbing them together in the second jar of sterile rinse water).  The swab tips are basically dripping with spore water after the rub and should have no problem transferring plenty into the grain directly.. the Gandalf baggie I made with the grains I directly swiped were clean enough to go to 2 flushes and get clean clone material:


I’m finding that the small amount of grain colonizes so fast that even a bit of bacteria doesn’t stop a baggie grow to get clone material and then it’s home free from there. 

I’ll update with how swabbing directly to grain goes (since I’ve only done it once but I don’t see how cutting out the syringe part would affect the outcomes that I’m already getting).. I’m gonna do pea+ f6 and yeti from swab next

Faht


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 26 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27512182 - 10/21/21 08:08 AM (2 years, 3 months ago)

I tried this the other day but what I did was take the plunger out and stick the barrel through a piece of cardboard to hold it up.


Then I got a second syringe full of sterile water, put the swab just inside the propped up barrel, and squirted it with the water. Once it was wet I stuck the syringe into the swab and kinda pulled it loose and then shot the rest of the water out from inside the cotton.


Then I pressed the swab against the side of the barrel to get the rest of the water out and put the plunger back in.


This stuff works great, I was really surprised the amount of spores that came off.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: A.k.a]
    #27512256 - 10/21/21 09:39 AM (2 years, 3 months ago)

:threadmonitor:


--------------------
Being pissed on does not make you a real man.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Guerrilla]
    #27522287 - 10/28/21 11:52 PM (2 years, 2 months ago)

So I made 2 pea+ f5 and 2 papu F2 glc jars where I dipped the wet swab directly into the grain jars on 10/24 and so far the pea+ has germed. 


My plan is to leave one of each to pin invitro and clone a pin to another grain jar.. like a plate pin and the other other with be GLC’d to jars as long as they look decent.. all will be spawned to cv in a baggie once “used”

Gotta good feeling about these new steps

:popcorn:

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27528315 - 11/03/21 02:28 AM (2 years, 2 months ago)

The swab “dip” jars are looking good a couple days after first shake.. shook them again right after pic.. should finish up pretty quick after they recover


Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27546229 - 11/16/21 07:32 PM (2 years, 2 months ago)

So far the dip has worked great.. fir the pea+ f6, both 4oz jars fully colonized and one was used to knock up 2 qts (shook them yesterday) and the other, I’m waiting to see invitro pinning to clone and for the papu f3, I used one of the jars for glc before it was fully colonized and looked really clean so I just tossed the grains from that one because they wouldn’t have survived being spawned but the other one I’m also waiting on invitro pins for cloning


The pea+ f6 glc grains were spawned to a baggie and colonized great so if the qts don’t work out, it looks like I’ll still get swabs to move onto f7, np


Once I get pin clones from each of the other jars, they’ll be spawned to baggies as well.. the papu qts looked great when I shook them so I think they’ll be a tub of them coming up but gotta love backup plans lol

Faht


Extras: Filter Print Post Top
InvisibleDrboomer
The lord magnificent
I'm a teapot User Gallery

Registered: 09/22/19
Posts: 957
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27547144 - 11/17/21 02:49 PM (2 years, 2 months ago)

Inspiring man
I've been getting swabs on tiny pf cakes by tearing bits off and burying them, then casing and fruiting for tissue samples. I don't think I'll bother trying to isolate on agar before fruiting again. The process moves much faster it's easier and have 15 or 20 projects at a time isn't a daunting task. If it pins invitro cause you couldn't get to it mission accomplished.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Drboomer] * 1
    #27564031 - 12/01/21 12:25 AM (2 years, 1 month ago)

The pea+ f6 sandwich baggie.. took f7 swabs just in case the tub doesn’t work out but it’s looking aight.  Also made the 2nd glc jars of both into sandwich bags.. neither grew pins invitro so I just spawned them. 


Papu f3 tub looks great too and the baggie should be knotting up soon.

So far not to shabby for dipping swab tips into grain.  Pretty fast too

Faht


Extras: Filter Print Post Top
Offlinekeeno
enthusiast
Male User Gallery


Registered: 06/01/11
Posts: 2,679
Loc: UK Flag
Last seen: 13 days, 13 hours
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27780488 - 05/16/22 03:48 PM (1 year, 8 months ago)

sorry for a complete noob question here
for the sterilsed water... can I just do the old "Pan of boiling water, suck up and expel the boiling water a load of times in a syringe. fill syringe. let it cool, then use that to wash the swabs in"?

sorry, been a long day :sosleepy:


--------------------
Check out my Psilocybe Cyanofriscosa (Allenii) TEK


Top fungi-knowlodgy with THE TRIBE!
THE TRIBE


Extras: Filter Print Post Top
InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: keeno]
    #27780491 - 05/16/22 03:50 PM (1 year, 8 months ago)

If that works in other circumstances like making spore syringes, which IIRC it does, then yes.


--------------------
Being pissed on does not make you a real man.


Extras: Filter Print Post Top
Offlinekeeno
enthusiast
Male User Gallery


Registered: 06/01/11
Posts: 2,679
Loc: UK Flag
Last seen: 13 days, 13 hours
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Guerrilla] * 1
    #27780494 - 05/16/22 03:51 PM (1 year, 8 months ago)

cheers brother :highfive:


Extras: Filter Print Post Top
OfflinekingboomerDiscord
smurfhouse archetect
Male User Gallery


Registered: 06/15/04
Posts: 739
Loc: Rocky Mountain High!!!! Flag
Last seen: 1 day, 13 hours
Re: Making spore syringes from swabs (I’m using an oven bag**optional) [Re: fahtster]
    #27957585 - 09/19/22 03:23 PM (1 year, 4 months ago)

Bumping this.  I did this in a SAB, but followed the RADS procedure.  So far so good!











First 2 are cakes I noced up with the syringe.  I also took some of the leftover solution and put it on agar.  I'm sold!


-Kingboomer


Edited by kingboomer (09/19/22 03:32 PM)


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,269
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) [Re: kingboomer]
    #27957663 - 09/19/22 04:21 PM (1 year, 4 months ago)

:headbanger:  I’ve made probably 30+ swab sets into SS since making this thread and besides the ones that I did in the beginning before I rinsed the swabs before transferring to the water I used for the SS, I’ve gotten almost all of them to fruit.. i don’t think I lost a single cake or 4oz grain jar.  At least to the point where I could make a sandwich bag grow and most went glc to qts

I think gills are cleaner than we think and there’s possibly some kind of physical natural design of cap and gills that keeps them free of airborne contamination (don’t quote me on that but it makes sense in my brain that a fruit would want only their spores in that area.. maybe through electrical charges or the aerodynamic makeup of the cap and gills).  I’ll always want a swab over a print… prints sit face up for too long for my liking


Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 38 minutes, 12 seconds
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) [Re: fahtster]
    #27957836 - 09/19/22 06:13 PM (1 year, 4 months ago)

Ok so this is my first time seeing this post and all I have to say is. What in the name of MacGyver did I just read?

Like damn... There's outside the box and then there's this


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* Oven bag in Oven?!?!?!?!?! wikedanjel 1,977 1 07/08/02 04:33 AM
by mycofile
* Attention Newbies-Make Spore Prints With Foil! Catzeye 22,157 1 02/20/02 01:54 PM
by psilocybinjunkie
* Oven Bags M_R_Shrooms 781 3 06/27/01 01:51 PM
by Scourge
* OVEN BAGS! Sterile 2,554 8 05/15/01 12:11 PM
by Myconoclastic
* my oven bag tek yersodum 2,715 2 08/23/01 04:50 AM
by NDK
* Re: oven bags?????? AldousHuxley 1,207 12 06/06/00 07:06 AM
by Anonymous
* Exploding Oven Bags effwun 2,503 8 10/28/01 11:39 AM
by Anonymous
* Oven Bag Tek G-nome 2,569 4 06/04/01 05:14 PM
by lateshroom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,764 topic views. 22 members, 229 guests and 71 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.05 seconds spending 0.01 seconds on 12 queries.