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Offlinegabbk
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Trouble recognizing healthy APEU myc on agar
    #26993131 - 10/19/20 02:41 PM (3 years, 3 months ago)

Hey guys!
Is there any possibility that the one at the right is healthy APEU mycelium?

What's confusing me is that it has a good homogeneous form, but it's strong bright white and dense, like cottonish, you can see no branches in between. And the white tone is the same as the other ones that clearly (or at least I assume) are contamination because of the lack of organization.
These are the other ones I'm talking about:


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OfflineBig_Dub
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Re: Trouble recognizing healthy APEU myc on agar [Re: gabbk]
    #26993160 - 10/19/20 02:58 PM (3 years, 3 months ago)

it looks ok. cant tell what variety it is just by looking at mycelium though. just make transfers and grow it out. good luck. :smile:


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OfflineRogerRabbitM
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Re: Trouble recognizing healthy APEU myc on agar [Re: gabbk] * 1
    #26993163 - 10/19/20 03:00 PM (3 years, 3 months ago)

It looks OK.  You need to start making transfers to new plates.  Grab a bunch of tiny pieces from the extreme leading edge of growth and place them on new plates.  It's usually at least a few transfers before you get much sectoring.  The strain isolation video might help you decide what to take.
RR


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Offlinesmoot
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Re: Trouble recognizing healthy APEU myc on agar [Re: RogerRabbit]
    #26993217 - 10/19/20 03:33 PM (3 years, 3 months ago)

Looks fine. Take a couple of transfers. LPEU and APEU are almost always tomentose when I run them.


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Offlinegabbk
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Re: Trouble recognizing healthy APEU myc on agar [Re: smoot]
    #26993267 - 10/19/20 04:09 PM (3 years, 3 months ago)

Thanks for the feedback guys!
I thought maybe for being APEU it could behave somewhat different than any other cube.
Very much appreciated!
I'll keep transfering in peace then!


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Offlinegabbk
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Re: Trouble recognizing healthy APEU myc on agar [Re: gabbk]
    #27029344 - 11/08/20 10:40 PM (3 years, 2 months ago)

I'm back again after a good couple transfers guys!
Now these four plates are really driving me crazy.
Are they mold or mycelium? Looking at the front they all seem really normal, but what caught my eye is the back. Looking close they look kind of disorganized... Like if the mycelium grew like a swirl (I know one of these 4 plates has a little contam going on there)
Front:

Back:
Plate 1:
Plate 2:
Plate 3:
Plate 4:

All of these plates came from this plate with antibiotic agar (amoxicilin+clavulanate 800mg/liter)


In the other hand I had many other plates that in spite of taking continuous transfers, they still looked very similar to the very first one of this thread (the bottom right one), so I threw one of them on grain and this is what I got:

This one seems to be healthy right? Or is there any mold undercover there? Maybe I'm getting a little paranoid on this one:lol:

Sorry for the long post. I wanted to make sure to get in all the important details


Edited by gabbk (11/08/20 10:56 PM)


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Offlinegabbk
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Re: Trouble recognizing healthy APEU myc on agar [Re: gabbk]
    #27029774 - 11/09/20 08:29 AM (3 years, 2 months ago)

Now this is really strange. Today plate number 4 started to grow towards the ceiling of the plate


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Edited by gabbk (11/09/20 08:29 AM)


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OfflineBig_Dub
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Re: Trouble recognizing healthy APEU myc on agar [Re: gabbk]
    #27030353 - 11/09/20 02:30 PM (3 years, 2 months ago)

ive never grown APE or PE, but my oyster mushroom mycelium will grow towards the top of the plate like that. never seen it with cubes, but i dont see it as being impossible


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split_by_nine said:
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do the right thing


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Offlinegabbk
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Re: Trouble recognizing healthy APEU myc on agar [Re: Big_Dub]
    #27030447 - 11/09/20 03:53 PM (3 years, 2 months ago)

Yeah! I know oysters grow upwards, but that's not a good sign with cubes for sure.
Heck! I miss my newbie luck lol. As time pass it's seems to be harder... I'm now encountering newer contams now than then when I just began to do agar and did everything with sloppy form! :lol:
The hardest thing is that some molds or bacteria can really go undercovered to the simple glance


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