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Offline76Salvia
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Question about grain for spore syringe inoculation
    #26992409 - 10/19/20 07:16 AM (3 years, 3 months ago)

Hello, im gonna start my first inoculation (with a spore syringe, btw) I know that spore syringe to grain its not the best option, but anyways.


My (probably subjective) question is:

I have here different kind of grains, and I dont know which one use.

1-Brown rice (maybe too mushy?)

2-WBS mix (without sunflower seeds or corn, but with some small colored stones that I don't know what they are, I guess some kind of bird nutrient or something like that).

3-Bird seeds (Like WBS, but with just 1 kind of grain, in spanish its called "Alpiste", in english, maybe alpist or something hahahaha)


From my ignorance, I suppose that the best is the third option, because the WBS mix has different types of grain, and each one would be hydrated in a different way.


In any case, the procedure for the second and third option would be the same, no?
Wash, remove floating grains,
hydrate for 24 hours (?), boil for apx 10 min and dry them, right?


Thank you!

:fabkiddance:


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InvisibleRoger Clemency
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Re: Question about grain for spore syringe inoculation [Re: 76Salvia]
    #26992416 - 10/19/20 07:31 AM (3 years, 3 months ago)

For wild bird seed I’ve been doing them no prep and it works well. 275g of WBS, 135-150g of water. Just put both in the jars and PC and then shake well when they come out.

Fooman’s method also works well - https://www.shroomery.org/forums/showflat.php/Number/8508523

I’m not sure what kind of seeds you have in those mixes though, do you have a pic or the name on the bag?


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Offline76Salvia
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Re: Question about grain for spore syringe inoculation [Re: Roger Clemency]
    #26992428 - 10/19/20 07:43 AM (3 years, 3 months ago)

Sure :mushroom2:

The "single grain" and the mix options:



Thank you!


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InvisibleRoger Clemency
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Re: Question about grain for spore syringe inoculation [Re: 76Salvia]
    #26992461 - 10/19/20 08:22 AM (3 years, 3 months ago)

Alpiste = canary grass. I’ve never used that or had it in any mix but user Gastronomicus or similar name said he has used it in a mix and was thinking about trying it by itself.

I’d try that stuff first but really all grain prep follows the same principle. Try to get as much water in as you can without ruining the structure of the grain. I don’t personally soak grains ever because it always grossed me out lol. I’d try the Fooman’s method probably or no prep with a few different times/ratios and see what works for that seed


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OfflineGastronomicus
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Re: Question about grain for spore syringe inoculation [Re: 76Salvia]
    #26992463 - 10/19/20 08:23 AM (3 years, 3 months ago)

Hola que tal?

I've been using alpiste as well, although I typically mix it with millet (mijo blanco o rojo). I do a 24 hour soak in water then strain for about 30 minutes and PC for 2 hours. Works well



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Offline76Salvia
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Re: Question about grain for spore syringe inoculation [Re: Gastronomicus]
    #26992492 - 10/19/20 08:39 AM (3 years, 3 months ago)

Hola! :smile:

So... do you think that just alpiste will work? Or better mix it with WBS (no sé donde encontrar mijo)

Thank you ~


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Offline76Salvia
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Re: Question about grain for spore syringe inoculation [Re: Roger Clemency]
    #26992497 - 10/19/20 08:42 AM (3 years, 3 months ago)

Oh, I see. Canary grass then hahaha
Yes, probably I will try something like the Fooman’s method.

Estimating the right amount of liquid to do it directly on the PC seems somewhat tricky.

Lets see


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OfflineGastronomicus
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Re: Question about grain for spore syringe inoculation [Re: 76Salvia]
    #26992512 - 10/19/20 08:55 AM (3 years, 3 months ago)

I haven't tried it yet but it seems to colonize fine. The WBS you showed looked good too though. Again I just soak overnight, strain, and PC. I don't boil.


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OfflineBig_Dub
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Re: Question about grain for spore syringe inoculation [Re: 76Salvia] * 1
    #26992519 - 10/19/20 08:57 AM (3 years, 3 months ago)

1) if you are starting from spores, BRF is the way to go imo

2) WBS is easy. you can do minimal prep (essentially, just soak it). Dont worry about removing corn or sunflower seeds etc; waste of time imo.

3) any time of bird seed will work

you might also want to use oats. if you use WBS, just rinse it a few times, soak it, load em into jars/bags and PC for 1.5-3 hrs at 15 pci


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Offlinesharkjazz
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Re: Question about grain for spore syringe inoculation [Re: 76Salvia]
    #27163651 - 01/22/21 02:10 PM (3 years, 24 days ago)

Quote:

76Salvia said:
Oh, I see. Canary grass then hahaha
Yes, probably I will try something like the Fooman’s method.

Estimating the right amount of liquid to do it directly on the PC seems somewhat tricky.

Lets see




Did you use alpiste / canary grass seed in the end? How did it go? That's what I'm planning to use once I can get a print / sample from my grow kit


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Offlinesharkjazz
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Re: Question about grain for spore syringe inoculation [Re: Gastronomicus]
    #27163655 - 01/22/21 02:11 PM (3 years, 24 days ago)

Quote:

Gastronomicus said:
Hola que tal?

I've been using alpiste as well, although I typically mix it with millet (mijo blanco o rojo). I do a 24 hour soak in water then strain for about 30 minutes and PC for 2 hours. Works well






what ratio alpiste / millet do you use? Haven't seen millet / mijo here in Ecuador so planning on just plain alpiste.


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OfflineGastronomicus
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Re: Question about grain for spore syringe inoculation [Re: sharkjazz]
    #27163775 - 01/22/21 03:13 PM (3 years, 24 days ago)

Fair warning, all the jars I used with alpiste molded once they got in shoe boxes. Could be bad spawn but I'm thinking the alpiste doesn't colonzie very well and is a vector for mold.


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Offlinesharkjazz
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Re: Question about grain for spore syringe inoculation [Re: Gastronomicus]
    #27168662 - 01/25/21 08:52 AM (3 years, 21 days ago)

thanks for the update. There goes that plan then. Will give a couple of jars ago as have the alpiste already and look for another option.

cheers


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OfflineProfessor X
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Re: Question about grain for spore syringe inoculation [Re: Big_Dub]
    #27168850 - 01/25/21 10:51 AM (3 years, 21 days ago)

Quote:

Big_Dub said:
1) if you are starting from spores, BRF is the way to go imo

2) WBS is easy. you can do minimal prep (essentially, just soak it). Dont worry about removing corn or sunflower seeds etc; waste of time imo.

3) any time of bird seed will work

you might also want to use oats. if you use WBS, just rinse it a few times, soak it, load em into jars/bags and PC for 1.5-3 hrs at 15 pci


In my experience sunflower seeds don't colonize correctly cause cubes don't care for the lignin but trich doesn't mind it a bit. Best to get all out you can unless you're doing 1 and dump.


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OfflineGastronomicus
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Re: Question about grain for spore syringe inoculation [Re: sharkjazz]
    #27169164 - 01/25/21 01:36 PM (3 years, 21 days ago)

PS I googled for millet in Ecuador, it's called mijo blanco. You should be able to find it in pet stores or order it from Mercadolibre :thumbup:


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OfflineBig_Dub
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Re: Question about grain for spore syringe inoculation [Re: Professor X]
    #27169210 - 01/25/21 02:05 PM (3 years, 21 days ago)

Quote:

Professor X said:
Quote:

Big_Dub said:
1) if you are starting from spores, BRF is the way to go imo

2) WBS is easy. you can do minimal prep (essentially, just soak it). Dont worry about removing corn or sunflower seeds etc; waste of time imo.

3) any time of bird seed will work

you might also want to use oats. if you use WBS, just rinse it a few times, soak it, load em into jars/bags and PC for 1.5-3 hrs at 15 pci


In my experience sunflower seeds don't colonize correctly cause cubes don't care for the lignin but trich doesn't mind it a bit. Best to get all out you can unless you're doing 1 and dump.





Ive heard this too. I used to take the seeds out, but i never noticed any difference. whether you take em out or not, its pretty easy to do.


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