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OfflineHikeadellic
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Registered: 08/31/20
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Agar consistancy issues
    #26991765 - 10/18/20 05:34 PM (3 years, 3 months ago)

Hey Shroomery! Success has been had with g2a on large plates. The agar was prepared in a 1 liter bottle. Equal parts BRF and agar powder in large quantities and a very small amount of instant yeast. The next two runs were prepared with 100 ml bottle same recipe except scaled down. The plates were very small compared to the first run.

The first run worked perfectly. The second run was s2a and did not germinate. The third run, prepared the same as the second run, was an attempted clone biopsy. No growth yet, but its only been a few days so I think more time maybe required.

I think the first run was more watery then the others so that might be the issue.

Where am I going wrong? How should I proceed?


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InvisibleBaba Yaga
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Registered: 09/13/20
Posts: 3,957
Loc: Hyperspace Chicken Coop
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Re: Agar consistancy issues [Re: Hikeadellic] * 1
    #26991776 - 10/18/20 05:45 PM (3 years, 3 months ago)

Your recipe is a bit vague, can you be more precise about your quantities?


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OfflineHikeadellic
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Re: Agar consistancy issues [Re: Baba Yaga]
    #26991803 - 10/18/20 06:07 PM (3 years, 3 months ago)

I did not note exactly what I used but I think it was about half a cup agar, half a cup brf, and just a little pinch of the yeast. Might have been 1/4 and 1/4. After cooking the 1L bottle, it was like a brick of agar in the middle and watery around it. The 2nd and 3rd times there was not a brick, however after pouring it seemed like it was stiffer after cooling then the first run.

The 100 ml recipe was scaled down to a maximum of 1/10 the cup, maybe closer to 1/20th.

Would it be better to look into other recipes like PDA, MEA, or grain soak water?


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OfflineChapuco
nice cock bro, nice tits miss
I'm a teapot User Gallery

Registered: 08/11/20
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Re: Agar consistancy issues [Re: Hikeadellic]
    #26991862 - 10/18/20 06:52 PM (3 years, 3 months ago)

you should take note of what are you doing, this way you won't succeed


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OfflineChapuco
nice cock bro, nice tits miss
I'm a teapot User Gallery

Registered: 08/11/20
Posts: 30
Last seen: 2 years, 8 months
Re: Agar consistancy issues [Re: Hikeadellic]
    #26991865 - 10/18/20 06:53 PM (3 years, 3 months ago)

btw try 500g soak water + 10g agar agar


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Nice to meet you. Follow me on Instagram. @admin.orissa
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OfflineHikeadellic
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Registered: 08/31/20
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Loc: Appalachian Trail
Last seen: 11 hours, 35 minutes
Re: Agar consistancy issues [Re: Chapuco]
    #26991880 - 10/18/20 07:03 PM (3 years, 3 months ago)

Ill try that next go around. Thanks for the tip :thumbup:


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