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OfflineFrrrunkis
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About oat jars - before spawning to bulk
    #26990964 - 10/18/20 03:49 AM (3 years, 3 months ago)

1. The quart jars I've currently got going are of this kind. Basically it's not possible to neatly slide the colonized substrate out. When it's time to spawn to bulk, what's the best way to get the contents out? I know for g2g transfers you basically shake shake shake violently until it's all loose, but how about spawning to bulk? My worry is that the mycelium will be too weak and prone to contamination before it recovers.

2. Is there a way to make sure the jar is fully colonized and ready? Is it enough if it looks like it from the outside? Also, does it have to be all thick cream-white mycelium (which I think is basically dense rhizo growth pushing against the glass), or does the mild semi-transparent growth count as colonized too?


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Literally nothing matters. This is fine.


Edited by Frrrunkis (10/18/20 03:55 AM)


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OfflineA.k.aM
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Re: About oat jars - before spawning to bulk [Re: Frrrunkis]
    #26990984 - 10/18/20 04:18 AM (3 years, 3 months ago)

With bulk you need to break the spawn up to mix it in the coir anyway so it won’t matter.

As long as there’s myc over all the grain it’s colonized but it sounds like you might have some bacterial spawn, pics would be good.


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LAGM2020


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OfflineFrrrunkis
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Re: About oat jars - before spawning to bulk [Re: A.k.a]
    #26991012 - 10/18/20 05:21 AM (3 years, 3 months ago)

The "thick white":


The "less dense":


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Literally nothing matters. This is fine.


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Offlinesuperbob57
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Re: About oat jars - before spawning to bulk [Re: Frrrunkis]
    #26991037 - 10/18/20 06:16 AM (3 years, 3 months ago)

I understand what he's getting at, he's worried about getting contamination going into a bulk substrate he's worried about touching mycelium itself he wants to not touch the contents whatsoever it sounds like, I'm guessing to lower it the chances of bacteria entering his grow. I've done what he's talking about where you shake the jar to break up the mycelium inside so you don't have to crumble it with your hands. But if you have a thick jar as shown in the picture above here and picture one. When the mycelium is that thick and dense it will not have any problems recovering into the spawn, Corior in this instance.
Original Opie are you worried about contamination or you having problems with bacterial contamination is this why you're asking these questions?
To make sure to sterilize your materials before working with them like vermiculite and others cleanliness is key


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InvisibleMunchauzen
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Re: About oat jars - before spawning to bulk [Re: Frrrunkis]
    #26991058 - 10/18/20 07:04 AM (3 years, 3 months ago)

Quote:

Frrrunkis said:
The "thick white":


The "less dense":




1st jar looks bacterial. 2nd jar looks totally healthy.

you can always put coir on top of the bottles and just let them fruit that way



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InvisibleNobler Hino
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Re: About oat jars - before spawning to bulk [Re: Munchauzen]
    #26991101 - 10/18/20 07:51 AM (3 years, 3 months ago)

Nice lol


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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
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OfflineFrrrunkis
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Re: About oat jars - before spawning to bulk [Re: A.k.a]
    #26991115 - 10/18/20 08:13 AM (3 years, 3 months ago)

Quote:

superbob57 said:
Original Opie are you worried about contamination or you having problems with bacterial contamination is this why you're asking these questions?
To make sure to sterilize your materials before working with them like vermiculite and others cleanliness is key



It's not really about touching vs not touching the spawn. Basically my logic is as follows.

When I want to do g2g, I shake the jar until the contents are loose and I can easily do a transfer without digging. My assumption is that the violent shaking weakens the mycelium, but since I'm trying to exercise good sterile technique, it won't have any significant competition and will therefore have time and space to recover.

When I want to use the jar for spawning to bulk, the weakened mycelium will have a lot of competition and I'm worried that it won't be able to recover quickly enough to dominate the substrate.

So my question is whether the process of "final" shaking of the jar before using the contents does indeed weaken the mycelium, and if so, does it weaken it significantly enough to pose a problem.

Quote:

A.k.a said:
With bulk you need to break the spawn up to mix it in the coir anyway so it won’t matter.



Right, but I'm assuming that the manual breaking up of spawn can be done much more gently and with less disruption to the mycelium than banging the jar against a tyre. Again, the crux of my dilemma is whether this actually makes any difference. Maybe the way I'm thinking about it is just plain wrong and the mycelium doesn't care?

Quote:

Munchauzen said:
Quote:

Frrrunkis said:
The "thick white":


The "less dense":




1st jar looks bacterial. 2nd jar looks totally healthy.

you can always put coir on top of the bottles and just let them fruit that way





I hope number 1 is not bacterial - if it is, I'll know soon. :/ Also wtf at that jar, can it actually produce any significant amounts of mushrooms?


--------------------
Literally nothing matters. This is fine.


Edited by Frrrunkis (10/18/20 08:15 AM)


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OfflineForresterM
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Re: About oat jars - before spawning to bulk [Re: Munchauzen]
    #26991151 - 10/18/20 08:43 AM (3 years, 3 months ago)

Quote:

Munchauzen said:
you can always put coir on top of the bottles and just let them fruit that way






Wow, nice results from a shit jar.  I'll have to try that!  :thumbup:


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OfflineA.k.aM
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Re: About oat jars - before spawning to bulk [Re: Forrester]
    #26991253 - 10/18/20 10:19 AM (3 years, 3 months ago)

It can take it, some of the teks here put myc in a blender or cheese grater and it recovers super quick.


That first jar is crazy looking with all the rhizo. Even if it is bacterial I’ve always gotten at least one flush out of sketchy jars.


--------------------
LAGM2020


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OfflineFrrrunkis
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I'm a teapot
Registered: 06/14/20
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Re: About oat jars - before spawning to bulk [Re: A.k.a]
    #26991311 - 10/18/20 11:07 AM (3 years, 3 months ago)

Quote:

A.k.a said:
some of the teks here put myc in a blender



Ok, yeah, that settles it. No amount of G-force will be as bad as a blender. Thank you.

Should I wait a few more days once all grain visible from the outside is covered, or is it not necessary?


--------------------
Literally nothing matters. This is fine.


Edited by Frrrunkis (10/18/20 11:21 AM)


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