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OfflineDhearic
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Registered: 01/24/14
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Re: Post Your New Tools Of The Trade. [Re: SHROOMSISAY01]
    #26989168 - 10/16/20 08:51 PM (3 years, 6 months ago)

Quote:

SHROOMSISAY01 said:
I built a high-pressure mist kit for a pressure washer that works awesome and hooks up with a garden hose easy as pie. With no cleaning of tubs. I sell a  mister kit for 10' x 5' room for $190 it is the best system out there way better than pond foggers and like I said no cleaning. My system has been going for 3 years and I have done absolutely nothing but let it run...






I like that.

:takingnotes:


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Credit where credit is due.

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OfflineGan
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Re: Post Your New Tools Of The Trade. [Re: BrownBear]
    #26989322 - 10/16/20 11:37 PM (3 years, 6 months ago)

Quote:

Iambrownbear said:
Would you say that enough time has passed on my grain bags and that my nose and eye test is adequate?





Nose and eye test for what? To tell if you sterilized your grain successfully? The one that has been sitting for a few weeks?

As for the eye and nose test, that will give you a decent idea of how clean they are. However, I will add a disclaimer and say that I've found some contams can be a little less pronounced on just straight grain. Like a very slight bacterial infection, for example. While a good sniff may be able to pick that up, it could also miss it. I've definitely had spawn that smelled fine, but I knew for a fact (either by the way it looked, the way it recovered from a shake, etc.) that it was bacterial to some extent. In my experience, the way I'll use the smell test when I use it is if I smell something then I definitely know something is up. Either a mold and/or bacteria infection. But if it smells clean, I don't use that as my only evidence that it's clean. It's just a secondary confirmation. If it passes the eye test, shake test, smell test, breaks up easily, and just has that look that comes with clean spawn then I feel pretty confident it is clean and good to go. But, sometimes I'm wrong even after confirming all that. Always learning. You could apply most of these concepts to your hydrated grain that hasn't been inoculated yet.

As for whether it is fine after 3 weeks, you'll find differing opinions here:

Some believe that, for the most part, when we sterilize our grain in the PC we more or less kill everything in the jar. If that is truly the case and your filter works as it should, then theoretically your jars are fine to use as long as the grain stays hydrated. It will dry out over time.

Others believe that with our sterilization procedures, we kill a large majority of potential contams, but we aren't able to kill absolutely everything (due to air pockets, endospores, other shit... I'm really not 100% read up on this so do your own research please). So, what we are achieving with our sterilization procedure is killing off almost everything so that we can give our healthy (emphasis on healthy) myc a headstart on any nasties. Simply put, once myc has full colonization on a source, it is very resilient and hardy. So, that camp would probably say that after 3 weeks, the small percentage of contams left over that weren't killed may have started to work their magic in those bags. So maybe you shouldn't use them.

Personally, I'm a little on the fence. I kept a jar once for 11 months after sterilization just cause... fuck it, why not. I wanted to see. It stayed clean, from what I could tell. But all I did was smell it and look at it, so maybe it wasn't.

If these are your only grain and you don't have any other spawn going at the moment, I'd say fuck it and go for it. Probably don't wanna put a trophy culture on it or something you don't have a backup of. But may as well use them while you have it. Just make sure you mark them well so you know those are the ones that sat a while. I've had grain sit for up to a month and I used it with success. However, that was only one time, so it's very possible I could have just gotten lucky or it actually was contamed and I didn't spot it.


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OfflineBrownBear
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Re: Post Your New Tools Of The Trade. [Re: Gan]
    #26989639 - 10/17/20 06:33 AM (3 years, 6 months ago)

Thank you for the well thought out response. You have given me a lot of things to consider. It will be some time before I am ready to transfer anything from agar to grain. I ran those grain bags just to see how good my grain prep and sterilization process are. I guess without actually inoculating them there is no sure fire way for me to know whether my techique was good enough or not. I'm not worried about using those bags and I plan on just tossing them.

Any suggestions on how to get the pc up to 15 psi? I guess that is where my real concern is because every grain prep thread I have read says to get the pc to 15 psi but mine max's out at 13.5 psi.

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OfflineA.k.aM
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Re: Post Your New Tools Of The Trade. [Re: BrownBear]
    #26989663 - 10/17/20 07:07 AM (3 years, 6 months ago)

You can always just cook the grain longer. Or tape coins to the rocker but that could be real sketchy depending on how it’s made.


--------------------
LAGM2020

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Offlinenmd_myco
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Re: Post Your New Tools Of The Trade. [Re: A.k.a]
    #26989920 - 10/17/20 11:06 AM (3 years, 6 months ago)

I know that on an AA you can replace the weighted steam port with a valve that can be opened and closed manually. My 50x (autoclave) comes with that valve installed and gets up to 20 psi easily.

Might not be safe to do with a cheap PC.

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OfflineBrownBear
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Re: Post Your New Tools Of The Trade. [Re: nmd_myco]
    #26990296 - 10/17/20 04:11 PM (3 years, 6 months ago)

Thank you all for the responses. I will just wait until I have a clean culture to put to grain and see where it goes from there. Increasing cook time if needed. Don't want to try anything dangerous, especially without good reason.

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Invisiblefungusmuncher
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Re: Post Your New Tools Of The Trade. [Re: BrownBear]
    #26991120 - 10/18/20 08:21 AM (3 years, 6 months ago)

I think I need to replace the control valve on my 25x, it starts leaking steam around 16psi and gets incredibly loud.  The newer control valves look a bit different (mine looks like a toggle switch) so I'm hoping there won't be any issues with the fit. 

Anyone here put a newer control valve onto an older AA sterilizer?

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OfflineGrimsweeper
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Re: Post Your New Tools Of The Trade. [Re: fungusmuncher]
    #26991254 - 10/18/20 10:19 AM (3 years, 6 months ago)

Yeah I put the new model valve on my 25x. Looks a little different but screws in the same. The one on the left is the new one.



--------------------
When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps

Edited by Grimsweeper (10/18/20 10:21 AM)

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Invisibletrippytimes
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Re: Post Your New Tools Of The Trade. [Re: Grimsweeper]
    #26991421 - 10/18/20 12:28 PM (3 years, 6 months ago)



Well went shopping and found this monster of a Crock-Pot! It's so huge I can run my substrate for a 66qt tub at 150°. So nice to pasteurized without thinking. Also made a new grain dump. New tools/toys. My 75x is coming soon. Thanks guys for all these tips I'm able to run grain easy set and forget my substrate is set and forget. I mean I can now run 20+ tubs in a weekend while doing laundry. Even my agar is done without even watching my stuff with my instapot. Btw everyone should have one! You can put your agar and syringes and scalpel in at the same time set and forget. Sorry if anyone lives around me and can't seem to find 66qt purple latch i have bought out big lots, target, walmart. Every store in 10 miles from me. Woohoo almost up to 30 tubs only need 20 more.


--------------------



want more info on shrooms "click"

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Offlinepureshrooming
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Re: Post Your New Tools Of The Trade. [Re: trippytimes] * 2
    #26998128 - 10/22/20 03:04 PM (3 years, 6 months ago)

Finally going with the flow over here!

I wanted to put something together in my garage that could be mostly discreet and let me keep all my lab activities in one cleanish space so I put together this



2x4 FFU in a 5x5 grow tent. I have a merv13 filter on the outside of the tent where air is pulled in through another filter by an inline fan. I am trying to source a 8x8 hepa filter for cheap but for right now this setup keeps positive pressure and at least filters the air a little bit. Not necessary but I like everything being enclosed in a tidy package and done with a minimal investment.

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OfflineBrownBear
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Re: Post Your New Tools Of The Trade. [Re: pureshrooming]
    #26998140 - 10/22/20 03:14 PM (3 years, 6 months ago)

I like the idea of grow tents for this purpose. I have a 4x8 that I am doing my sterile work in. I also have a 5x5 for my spawn bags and tubs. I have had tents setup in my basement for almost 2 1/2 years. I hadn't used them for about six months and was wondering whether I should take them down. Then it hit me. How about I grow some mushrooms. :laugh:

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Offlinenmd_myco
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Registered: 09/05/20
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Re: Post Your New Tools Of The Trade. [Re: pureshrooming]
    #26998214 - 10/22/20 03:56 PM (3 years, 6 months ago)

Quote:

pureshrooming said:
Finally going with the flow over here!





2x4 FFU in a 5x5 grow tent. I have a merv13 filter on the outside of the tent where air is pulled in through another filter by an inline fan. I am trying to source a 8x8 hepa filter for cheap but for right now this setup keeps positive pressure and at least filters the air a little bit. Not necessary but I like everything being enclosed in a tidy package and done with a minimal investment.




Very slick build.  I once had a pair of 128 site aero cloners in a tent that felt like that. No flow hood but inline "True HEPA" (not medical grade like FFUs for mycology) on the room intakes- plenty good for plant propagation- and positive pressure.  The maintenance of that pressure was a bit sloppy if I wasn't careful with the zip doors. But you cant beat the cost to buy yourself some truly clean space.

I never did like to grow in them... so hard to keep them clean when growing mediums get involved, unless you can fully break it down and clean properly between runs.

If I don't get off my ass and start building some walls in here, I may just go the tent route temporarily... Heating the bigger environment is starting to be costly.

One of my issues is that I am trying to balance the room air intake with the Flow Hood's... if you create too much positive pressure in the room, does it push air into the hood's environment? the hood is sucking out of that internal environment... questions for an HVAC guy! Don't want to be forcing less clean air into my fully sterile environment...

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Offlinepureshrooming
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Re: Post Your New Tools Of The Trade. [Re: nmd_myco]
    #26998351 - 10/22/20 05:14 PM (3 years, 6 months ago)

I’ve been wondering those same questions and I’m just going to see how it goes. I poured some plates for my first run so I’ll let those chill for a while and hopefully nothing pops up.

I played with the intake fan speed a bunch before I did any work. It doesn’t feel like there is much turbulent air so far but I don't actually know haha. I did the lighter test on my hood and its consistent at every point still. I thought about sealing all the holes and just running the hood but using ISO and having a butane burner on didn't sound like a great idea for a small sealed space.

It isn't "clean room" status but I figure at the very least it is filtering some of the air before it hits the hood and maybe extend the life of the filter. Plus I can open my garage and nobody thinks twice about a grow tent in the region I'm in.

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Offlinenmd_myco
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Re: Post Your New Tools Of The Trade. [Re: pureshrooming]
    #26998389 - 10/22/20 05:43 PM (3 years, 6 months ago)

Is the intake for the hood inside the tent or outside?

with a space that small, you could just route the intake for the HEPA as the only intake. whats the CFM on that blower? Certainly it will create positive pressure within the tent.

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Offlinepureshrooming
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Re: Post Your New Tools Of The Trade. [Re: nmd_myco]
    #26998412 - 10/22/20 05:55 PM (3 years, 6 months ago)

intake for the hood is on the inside. The idea is that it is pulling already filtered air to reduce the load on the hepa filter. I'm also leaving the inline fan on all the time to keep positive pressure in the tent even when the hood is off.

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Offlinenmd_myco
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Re: Post Your New Tools Of The Trade. [Re: pureshrooming]
    #26998455 - 10/22/20 06:18 PM (3 years, 6 months ago)

disregarding the deterioration of your flow hood filter, i'm betting in such a small space, if you were to run the Flowhood ducted to the outside (blower cfm?) I think its would work the best.. granted, you'd be running the flowhood 24/7 to get your positive pressure. and now worries about turbulent air or blowback as long as you have enough exhaust to compensate with a differential for the appropriate amnount of positive pressure.

If your intake fan is a higher CFM than the flowhood, its got to be putting pressure on your sterile environment, unless there is appropriate relief by exhausting...

how far from your flow hood did you hold the lighter?

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InvisibleTedsDead
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Re: Post Your New Tools Of The Trade. [Re: pureshrooming]
    #26998459 - 10/22/20 06:20 PM (3 years, 6 months ago)

Quote:

pureshrooming said:
Finally going with the flow over here!

I wanted to put something together in my garage that could be mostly discreet and let me keep all my lab activities in one cleanish space so I put together this



2x4 FFU in a 5x5 grow tent. I have a merv13 filter on the outside of the tent where air is pulled in through another filter by an inline fan. I am trying to source a 8x8 hepa filter for cheap but for right now this setup keeps positive pressure and at least filters the air a little bit. Not necessary but I like everything being enclosed in a tidy package and done with a minimal investment.



:homerdrool:  maybe one day when I own a house.  I really want a table like that tho!


Edit:  lol!  Its a grow tent:lol:  i thought u had a baller silver room.  Ok, just the table will do but i need real doors for an intake fan to be of use


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

Edited by TedsDead (10/22/20 06:22 PM)

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Offlinepureshrooming
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Re: Post Your New Tools Of The Trade. [Re: nmd_myco]
    #26998558 - 10/22/20 07:27 PM (3 years, 6 months ago)

Quote:

nmd_myco said:
disregarding the deterioration of your flow hood filter, i'm betting in such a small space, if you were to run the Flowhood ducted to the outside (blower cfm?) I think its would work the best.. granted, you'd be running the flowhood 24/7 to get your positive pressure. and now worries about turbulent air or blowback as long as you have enough exhaust to compensate with a differential for the appropriate amnount of positive pressure.

If your intake fan is a higher CFM than the flowhood, its got to be putting pressure on your sterile environment, unless there is appropriate relief by exhausting...

how far from your flow hood did you hold the lighter?




The intake can is only 400cfm and I have it turned down to 3/10 on the speed controller. Basically just enough to maintain positive pressure with both exhaust flaps open at the bottom of the tent. I'm for sure open to different ideas but I don't really want to run the hood 24/7 to maintain the positive pressure.

I can hold the lighter out as far as the table goes and it maintains 90. I can check that again to confirm. My future plans were to cut some plexi-glass to frame the sides and top of the filter.

TedsDead - yeah just a simple setup, still renting for now too. I have big plans when we finally decide where we want to settle down and buy. That table was only $150 on prime day!

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Offlinenmd_myco
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Re: Post Your New Tools Of The Trade. [Re: pureshrooming]
    #26998654 - 10/22/20 08:44 PM (3 years, 6 months ago)

I mean, the lighter don't lie. if it works, it works. with a fan speed controller on the intake, seems like you must have it balanced out.

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InvisibleWall.E
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Re: Post Your New Tools Of The Trade. [Re: nmd_myco]
    #27003120 - 10/25/20 04:49 PM (3 years, 6 months ago)

I asked in general discussion but maybe it's better suited here.

Are there any good filter and blower recommendations for something smaller, like a 12"? I don't work with bags, I'm an idiot and can't figure them out, so I just keep things small and work with at max 30 plates at a time. I wanna be able to move it easily, but I can do my own woodwork for that. I just wanna know what filter and blower combos people are using because I don't wanna buy from fungi perfecti but they're the only ones that make it easy to see what the fuck you're getting.


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Life’s shit, but I’m loving it


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