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Invisiblejosbos
Registered: 02/16/20
Posts: 134
alternative for petri dishes
    #26987560 - 10/15/20 07:18 PM (3 years, 3 months ago)

Hi there,

i want to know if i can use ketchup cups as a alternative for petri dishes. because i have seen videos on YouTube. where by people use ketchup cups instead of petri dishes. do anyone know if ketchup cups come sterile?


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Invisibleeatyualive
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Re: alternative for petri dishes [Re: josbos] * 2
    #26987563 - 10/15/20 07:22 PM (3 years, 3 months ago)

Lookup mr aliens holy grail plates and pastywhytes easy agar.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: eatyualive]
    #27018962 - 11/03/20 09:10 AM (3 years, 2 months ago)

ok, i looked those two up but still got some questions.

these are the cups that i got: (i placed a pen there for scale)




1. so i measure the ingredients than place them in a bowl and put hot boiling water to it and mix it right? (making agar)

2. than i pour a little layer of the liquid in those cups? (the cups are not sterilized)

3. than i wrap the top of the lid of those cups with foil and stack them inside my PC, i make sure the water inside the PC is not touching the cups be elevating the cups, and i sterilize them for 45 minutes at 12psi?

4. with Pastywhyte's Easy Agar Tek, he drills holes inside the lid of the cups, do i need to do that with my cups? (since my cups are a little bit different than the ones he uses)

5. i want to have the liquid colored blue, what product should i use? (can you give me a brand name)


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: josbos]
    #27018967 - 11/03/20 09:11 AM (3 years, 2 months ago)

ok, i looked those two up but still got some questions.

these are the cups that i got: (i placed a pen there for scale)




1. so i measure the ingredients than place them in a bowl and put hot boiling water to it and mix it right? (making agar)

2. than i pour the liquid in those cups? (the cups are not sterilized)

3. than i wrap the top of the lid of those cups with foil and stack them inside my PC, i make sure the water inside the PC is not touching the cups be elevating the cups, and i ster


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Invisiblegrownright
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Re: alternative for petri dishes [Re: josbos]
    #27019030 - 11/03/20 09:34 AM (3 years, 2 months ago)

I do not see those making it through a PC cycle. Like at all


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: grownright]
    #27019034 - 11/03/20 09:36 AM (3 years, 2 months ago)

they can be heated up to 118C/244,4F


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: grownright]
    #27019041 - 11/03/20 09:38 AM (3 years, 2 months ago)

These sauce and salad bowls are mainly used for tapenades, filet americain, egg salads and the like, but can also be used to store non-food products such as screws and nails. PP, or polypropylene, can withstand a temperature of up to 120 degrees Celsius and can be used in the oven as well as in the microwave.

Source:
https://www.disposablediscounter.com/sauce-and-salad-container-rectangular-108mm-125cc


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OfflineA.k.aM
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Re: alternative for petri dishes [Re: josbos]
    #27019056 - 11/03/20 09:48 AM (3 years, 2 months ago)

Those are pretty big.

I’ve seen quite a few people using the little circle ketchup things and just pouring them like regular plates.


As long as yours say 5 in the triangle on the bottom they should work in the pc.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: A.k.a]
    #27019083 - 11/03/20 10:15 AM (3 years, 2 months ago)

i just have a regular PC, so my PC is not big, i can place two side by side and stack them that way.



Of course i will use foil and such





what do you mean with: As long as yours say 5 in the triangle?


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OfflinePrincessRosalina
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Re: alternative for petri dishes [Re: josbos]
    #27019185 - 11/03/20 11:28 AM (3 years, 2 months ago)

There would be a number 5 in the triangle on the bottom of the container. It means that it is a type of plastic that can be pressure cooked.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: PrincessRosalina]
    #27019190 - 11/03/20 11:33 AM (3 years, 2 months ago)

Thanks for the reply! :-) , it got this logo:



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InvisibleSmartattack
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Re: alternative for petri dishes [Re: josbos]
    #27019248 - 11/03/20 12:02 PM (3 years, 2 months ago)

The problem is, a lot of things are PP5 for the container itself but not the lid. So you'll end up with a layer of thermo formed plastic in your vessel after PC.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: Smartattack]
    #27019256 - 11/03/20 12:05 PM (3 years, 2 months ago)

ok, so can i use 70% iso instead of PC ?


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OfflinePrincessRosalina
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Re: alternative for petri dishes [Re: Smartattack]
    #27019286 - 11/03/20 12:18 PM (3 years, 2 months ago)

Quote:

Smartattack said:
The problem is, a lot of things are PP5 for the container itself but not the lid. So you'll end up with a layer of thermo formed plastic in your vessel after PC.




I forgot to state this as well.

no, you still need to PC things.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: PrincessRosalina]
    #27019291 - 11/03/20 12:21 PM (3 years, 2 months ago)

but if i place those cups inside a PC i will get a layer of thermo formed plastic.

How can i sterilize those ketchup cups? any tips for me?


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Invisiblecoversall
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Re: alternative for petri dishes [Re: josbos]
    #27019564 - 11/03/20 02:21 PM (3 years, 2 months ago)

I've used the same jars I did BRF cakes in for no pour agar.



Got pan cambos germing atm.


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..:: How I Panaeolus. From Agar to Tea ::..


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: coversall]
    #27019570 - 11/03/20 02:23 PM (3 years, 2 months ago)

interesting


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Invisiblecoversall
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Re: alternative for petri dishes [Re: josbos]
    #27019598 - 11/03/20 02:31 PM (3 years, 2 months ago)

Not the easiest things to work in, but they were there and free. More than ok for germing spores.


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OfflineA.k.aM
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Re: alternative for petri dishes [Re: coversall]
    #27019642 - 11/03/20 02:54 PM (3 years, 2 months ago)

The ketchup cups come sterile in the bags I guess.

I’m almost out of plates so I’ll prob try them next just to see, plus there perfect size for dropping the whole puck into a jar.


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Invisiblecoversall
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Re: alternative for petri dishes [Re: A.k.a]
    #27019651 - 11/03/20 02:56 PM (3 years, 2 months ago)

Yeah I really wanted them to work, and some did.



But out of the different containers and agar teks I tried I found no-pouring in the sauce pots to be the least contam resistant. YMMV by all means. If you were in the uk I'd send you this box of 500 sauce pots I have for cheap!


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..:: How I Panaeolus. From Agar to Tea ::..


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: Smartattack] * 1
    #27019658 - 11/03/20 02:59 PM (3 years, 2 months ago)

Well i just PC them 40 minutes at 12psi, they still the same shape. i can make pictures


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OnlineLogicaL ChaosM
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Re: alternative for petri dishes [Re: josbos]
    #27019669 - 11/03/20 03:03 PM (3 years, 2 months ago)

Do u wrap them in parafilm? I feel like parafilm would protect the plastic pretty well.


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Invisiblemushhead
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Re: alternative for petri dishes [Re: coversall] * 1
    #27019692 - 11/03/20 03:12 PM (3 years, 2 months ago)

Honestly I just stick with either the short widemouth mason jars or pre-sterilized dishes.
I have glass dishes that I use for master cultures which I sterilize and then pour.
I wrap them all with parafilm because who wouldn't?
Some people use tape which isn't at all recommended. I used to swear by micropore as a good item to wrap plates with but ime it truly is not.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: LogicaL Chaos] * 1
    #27019697 - 11/03/20 03:15 PM (3 years, 2 months ago)

no i did not wrapped them, or used foil. i just did a dry run. empty cups with a little bit of water (450ML) inside the PC


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: alternative for petri dishes [Re: mushhead]
    #27019702 - 11/03/20 03:17 PM (3 years, 2 months ago)

yeah, but i bought 1000 cups for 45 euro, so i need to make this work


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