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Josex
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979578 - 10/11/20 07:56 AM (3 years, 4 months ago) |
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I don't use stirrers because I don't need to, the extra time is not an issue if you have a good rotation going. I'm not saying people should not test their LC's, I'm not here to be politically correct or trying to make people do what I do, I'm here to share what I do, among other things. I could perfectly test them but I simply don't need to and you'd understand it if you did what I do.
For starters, 0.2% strength in an LC is already more nutrition than myc can use. Using 2% is pretty much worthless, this is not agar. That extra LME is there to cloud the broth, little else.
Edited by Josex (10/11/20 08:03 AM)
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trippleblack
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26979593 - 10/11/20 08:08 AM (3 years, 4 months ago) |
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Quote:
Josex said: I don't use stirrers because I don't need to, the extra time is not an issue if you have a good rotation going. I'm not saying people should not test their LC's, I'm not here to be politically correct or trying to make people do what I do, I'm here to share what I do, among other things. I could perfectly test them but I simply don't need to and you'd understand it if you did what I do.
For starters, 0.2% strength in an LC is already more nutrition than myc can use. Using 2% is pretty much worthless, this is not agar. That extra LME is there to cloud the broth, little else.
i didn't mean 2% i meant .2% 2 teaspoons of powdered media per 500 ml is standard, thats within range by weight.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979897 - 10/11/20 11:56 AM (3 years, 4 months ago) |
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Josex, I prefer higher nute ratio because i've done tests and found they colonize faster and end up with more myc. Obviously, more than one (or two or three or four) tests must be done to find a semi definitive answer but that's what i have concluded so far.
I prefer to stir because i find they colonize much more quickly and end up being thousands of tiny myc fragments as opposed to a clump of myc. I like that better.
On your point of timing not mattering if you have a proper cycle going; I completely agree.
On the topic of being able to visually ID the cleanliness of low nute non stirred LC; I simply cannot speak because i don't have enough experience. I'd love to make a few up and see how it goes.
As far as whether or not it's necessary to test LC on agar; No, it's absolutely not. I prefer to because i've done it so much that it literally only takes a minute. I like to get my cycle rolling in such a way that it's just one more thing i throw into a sterile sesh. The time it takes to wipe down the jar, flame the loop and streak the plate is almost nothing.
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Josex
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Maybe myc can utilize better that extra nutrition if you stir the LC. 
I did sides by sides long ago doing LME broths that ranged from .2% to 3% strength and didn't notice much of a difference in growth if at all, but I wasn't using stirrers so... I posted the results in my LC thread iirc. Truth be told, the reason I don't use stirrers anymore is because I have a freaking steel tube in the jar that goes down to the bottom.  I prefer to use syringes over pouring these days so I'm fine with my freak ass jars and no stirrers. The tube helps break the myc just fine.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26979939 - 10/11/20 12:24 PM (3 years, 4 months ago) |
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interesting.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26982032 - 10/12/20 05:55 PM (3 years, 4 months ago) |
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Quote:
verum subsequentis said: I prefer higher nute ratio because i've done tests and found they colonize faster and end up with more myc. Obviously, more than one (or two or three or four) tests must be done to find a semi definitive answer but that's what i have concluded so far.
Quote:
Josex said: I did sides by sides long ago doing LME broths that ranged from .2% to 3% strength and didn't notice much of a difference in growth if at all
This is kind of interesting that the three of us ended up with different conclusions testing the same hypothesis. When I did my testing I used 0.1%, 0.2%, 0.4%, 0.8% and 1.5% LME and in a way I came to similar conclusion as Josex because after a 25 day incubation all LC's seemed to have grown a similar amount of mass. Tho there was a significant difference in growth speed during the first 3weeks.
Below are comparisons between 0.1% 0.2% 0.8% and 1.5%
Day 12 of incubation
   
Day 18
   
Day 25 0.1%, 0.2%, 0.8%, 1.5%
   
Now Im curious about what kind of broths you've tested Verum and how significant were the differences in colonization in your experience?
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26982050 - 10/12/20 06:02 PM (3 years, 4 months ago) |
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all my tests on done with stir plates. I think that makes a big difference.
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Forrester
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26982056 - 10/12/20 06:06 PM (3 years, 4 months ago) |
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Mateah you do some great work in LC there, nice pics to match with the data. Love it.
I can't believe the difference in looks once you go over .5%! I may be PM'ing you with questions soon when I get back into growing, it's been a while for me but I love LC and don't keep shit for records
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26982076 - 10/12/20 06:19 PM (3 years, 4 months ago) |
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Thanks dude yeah visually comparable results definitely facilitate the insights about it more clearly
And also I made 15 LC's today with 0.05% LME, the broth is very clear and I just hope the colonies won't be negatively affected by this extremely low nutrient density. I don't wanna spoil any more but I'm hoping I have some nice results to post in a coming experiment thread soon
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trippleblack
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26982267 - 10/12/20 07:55 PM (3 years, 4 months ago) |
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I use 2 grams per 1000 ml.. I love the pretty clear solution, my lc looks nothing like that, may try your recipe out. I use LME and a dash of soy peptone.
these are recent.. ready in 7 days. I usually test it on agar on about day 5.

the longer these lc sit, the more clear the solution get, looks like the mycelium eats up everything. not all cultures look the exact same in lc, these cordyceps like to spread around whole; the actives all collect at bottom(for these culutes anyway).
Edited by trippleblack (10/12/20 08:21 PM)
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26982471 - 10/12/20 09:49 PM (3 years, 4 months ago) |
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Quote:
trippleblack said: I love the pretty clear solution, my lc looks nothing like that
You just reminded me that I should probably make another set of LC's but from rye water this time. It makes for a very clear broth no matter what but if diluted some then it's definitely clearer than even 0.1% LME I'd say. Well see soon.
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26986814 - 10/15/20 10:48 AM (3 years, 3 months ago) |
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Oh dear I've started something havent I 😂
Thanks for everyone putting in their input
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26986843 - 10/15/20 11:08 AM (3 years, 3 months ago) |
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You did 
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Forrester
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26987213 - 10/15/20 02:39 PM (3 years, 3 months ago) |
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Quote:
Clc420 said: Oh dear I've started something havent I
It's almost guaranteed when you mention LC.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26987243 - 10/15/20 02:51 PM (3 years, 3 months ago) |
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26987269 - 10/15/20 03:08 PM (3 years, 3 months ago) |
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Quote:
Forrester said:
Quote:
Clc420 said: Oh dear I've started something havent I
It's almost guaranteed when you mention LC.
😂🙈 I wont bring it up in future haha
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