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Offlineankylosaurus
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Registered: 10/15/20
Posts: 5
Last seen: 2 years, 6 months
Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice
    #26986803 - 10/15/20 10:39 AM (3 years, 4 months ago)

Golden teacher, grain spawn bulked to coir/verm/gypsum in this plastic plant container(because its what I had). Colonization went smoothly. Once it was nearly fully colonized I transferred the pot into a monotub(lid upside down) for fruiting conditions, and misted occasionally.Seems theres a clearance zone on the surface that never got colonized that I think is due to some contaminant. This clearance zone is surrounded by yellow metabolites. There is even a line of metabolites over the surface, and the myc under it is a slightly different shade. I've absorbed some of the metabolites a few times with a napkin once it got too much, but theres been no improvement over the last 5-6 days. The amount of metabolite seems to have increased. Doesnt smell bad or anything though.

Is it fucked or can I still expect to get some mushrooms out of this?

This is my first time as a grower and I'm not exactly sure what to do. other relevant information might be avg temp here fluctuates between 28° / 19° C, it receives diffuse lighting, and I try not to overmist



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OfflineCamera93
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I'm a teapot


Registered: 08/15/18
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Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: ankylosaurus]
    #26986842 - 10/15/20 11:07 AM (3 years, 4 months ago)

Not looking to hot, I wouldn't expect much from this

how did you inoculate the grain spawn? Spore syringe to grain or any agar work?

my best guess at the moment is that your spawn wasn't clean.

Get started on other projects, start them here on the boards and plenty of people will be able to help you along

Welcome to the Shroomery :cheers:


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineSlushrooms
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Registered: 09/16/18
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Last seen: 4 months, 15 days
Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: Camera93]
    #26986981 - 10/15/20 12:30 PM (3 years, 4 months ago)

Slightly related, but I've had similar temperature swings while waiting for my permanent space (and environmentally stable) to be set up. It's contributed to major condensation issues (contams) and caused the substrate to loose enough moisture to shrink away from the sides resulting in side pinning, which doesn't rectify after dunking.


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OfflineBig_Dub
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Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: Slushrooms]
    #26986997 - 10/15/20 12:34 PM (3 years, 4 months ago)

Yeah that tub looks gross. need to get some cleaner spawn. there is mold everywhere.

doesnt look like metabolites to me, but I may be wrong.

I would toss and try again, being more careful with your technique and procedure :smile:


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split_by_nine said:
click me you fuck


do the right thing


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Offlineankylosaurus
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Registered: 10/15/20
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Last seen: 2 years, 6 months
Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: Camera93]
    #26987046 - 10/15/20 01:01 PM (3 years, 4 months ago)

Got it. I did agar work with an SAB at home. My plates looked clean though, so you're probably right about it being the spawn. However, the fully colonized grain spawn jar looked pretty clean, smelled good too. idk.
Gonna leave this in the corner and hope it resolves itself. probably occasional misting. Thanks for your reply.

I've got 4 completely colonized jars ready now, so I can probably start another bulk grow with that.

Considering a few things-
1) Directly fruit one of the jars in an FC to get my first taste of cubes, and make another coir pot from the rest.
2) Put all 4 jars to bulk in a pot

3)If I used 4 jars to spawn to bulk directly in the monotub in a 1:1 ratio, the substrate height would probably be 1.5-2 inch max. On the other hand, if I partition the tub using some carboard, and only have the grow on one half, I could get sub depth between 3 and 4 inches, which is ideal according to what I read. Which way would you recommend I do it?

I just really want something to work. :smile:


Edited by ankylosaurus (10/15/20 01:12 PM)


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OfflineBig_Dub
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Registered: 01/12/11
Posts: 2,700
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Last seen: 11 hours, 34 minutes
Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: ankylosaurus]
    #26987130 - 10/15/20 01:54 PM (3 years, 4 months ago)

when in SAB spray down with soapy water, let things settle. work with intention- be mindful of where your hands are: dont ever put non sterile items over sterile items; dont create lots of air motion, etc

contams on agar can be hard to see.

honestly dont overthink it. if i were in your situation, I would spawn 1:1 myc:coir in a shoebox. (lookup shoebox tec if you dont know what this is). I like to add a little casing layer too, but that's not totally necessary.

fruiting in a jar with a plastic bag over the top to act as a FC will work, but you wont get great yields.


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split_by_nine said:
click me you fuck


do the right thing


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Offlineankylosaurus
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Registered: 10/15/20
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Last seen: 2 years, 6 months
Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: Big_Dub]
    #26987964 - 10/16/20 05:42 AM (3 years, 3 months ago)

alright, i'll go get a plastic box for shoebox tek. Thanks for the SAB tips, ill keep those in mind. :smile:

A general question though, does it matter if the box is opaque or not, as long as it gets enough light from the top?

I'm gonna go ahead with one jar in FC and rest to bulk, so I can actually get something out of this, In case my second attempt at bulk flops too.


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OfflineBig_Dub
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Registered: 01/12/11
Posts: 2,700
Loc: Los Angeles Flag
Last seen: 11 hours, 34 minutes
Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: ankylosaurus]
    #26988131 - 10/16/20 09:33 AM (3 years, 3 months ago)

You'll soon find that cubes basically grow themselves. You don't need an opaque box, but cubes do benefit from light at all stages.

I like shoeboxes as a middle ground between cakes/jar fruiting and monotubs. Especially if i am trying to fruit something questionable.IMO, better to lose a quart in a shoebox than to lose 7 in a mono


--------------------
split_by_nine said:
click me you fuck


do the right thing


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Clearance zone with metabolites formed on my shoebox/pot. Likely contam.Please offer advice [Re: Big_Dub]
    #26988151 - 10/16/20 09:53 AM (3 years, 3 months ago)

You should post pictures of the spawn as it progresses, one of the hardest things to learn for me is telling clean spawn from dirty.


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LAGM2020


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