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 Clc420
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 Re: mycelium growing out very transparent  [Re: Mycolorado]
#26989913 - 10/17/20 11:00 AM (3 years, 5 months ago) |
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Quote:
Mycolorado said:
8 grams to what volume of media? Percent is the needed info. 2% nutes is pretty standard.
Sorry should of said. 500ml water 10g agar 8g lme
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 Mycolorado
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Re: mycelium growing out very transparent [Re: Clc420]
#26989916 - 10/17/20 11:03 AM (3 years, 5 months ago) |
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Hmmm. That’s 1.6% so may not be the issue. Super hard media could also be at play. I know some brands such as telephone are like glass at 2%.
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bodhisatta  
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Re: mycelium growing out very transparent [Re: Mycolorado]
#26989927 - 10/17/20 11:13 AM (3 years, 5 months ago) |
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Lab grade agar has a higher gel strength than telephone. Telephone is under 500g/cm but most lab grade is over 1000g/cm some are like 1600g/cm
Lab grade media is usually used at more like 15% but most of us using food grade stuff go with 20%.
But agar thats hardly solid and agar that's hard as a rock all work well. Bacteriological agar comes out extremely stiff mostly to keep colonies nice and pinpoint.
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Mycolorado
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Re: mycelium growing out very transparent [Re: bodhisatta]
#26989929 - 10/17/20 11:15 AM (3 years, 5 months ago) |
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Just spitballing  Who’s using agar at 20%?
Edited by Mycolorado (10/17/20 11:22 AM)
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Nichrome
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Re: mycelium growing out very transparent [Re: Mycolorado]
#26990011 - 10/17/20 12:13 PM (3 years, 5 months ago) |
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I run 3-4% nutes and see this type of growth sometimes. I run heavy on the nutes and the plates seem to last a lot longer on the shelf and pin more frequently but there is definitely some weird growth patterns with higher carbo content. That's all well and good for me but weird growth to the untrained eye is often precursor for alarm so as mentioned above keeping it around 2% is ideal for "standard" Rhizo/tom cube growth.
Personally I like to hunt mutant phenos.
I have also seen more growth like this when the sugars used are more caramelized, but that's just ME and I traded mycology as a "science" for mycology as an "artform" many years ago and haven't looked back.
Personally I'd transfer some pieces to new plates and see what happens.
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verum subsequentis
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Re: mycelium growing out very transparent [Re: Nichrome]
#26990012 - 10/17/20 12:15 PM (3 years, 5 months ago) |
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misplaced decimal error? 1.5% and 2%
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Clc420
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Re: mycelium growing out very transparent [Re: Nichrome]
#26990058 - 10/17/20 12:46 PM (3 years, 5 months ago) |
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Quote:
Nichrome said:
I run 3-4% nutes and see this type of growth sometimes. I run heavy on the nutes and the plates seem to last a lot longer on the shelf and pin more frequently but there is definitely some weird growth patterns with higher carbo content. That's all well and good for me but weird growth to the untrained eye is often precursor for alarm so as mentioned above keeping it around 2% is ideal for "standard" Rhizo/tom cube growth.
Personally I like to hunt mutant phenos.
I have also seen more growth like this when the sugars used are more caramelized, but that's just ME and I traded mycology as a "science" for mycology as an "artform" many years ago and haven't looked back.
Personally I'd transfer some pieces to new plates and see what happens.
If I'm running 500ml 10g agar how many grams of lme would he 2% ?
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Mycolorado
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Re: mycelium growing out very transparent [Re: Clc420]
#26990068 - 10/17/20 01:02 PM (3 years, 5 months ago) |
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10
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verum subsequentis
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Re: mycelium growing out very transparent [Re: Mycolorado]
#26990077 - 10/17/20 01:11 PM (3 years, 5 months ago) |
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calculators help when you don't know what to do. Also google makes quick work of most simple math.
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Nichrome
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1 ml of water equals 1 gram.
1 gram per 100 ml is 1%
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Where have all the people gone my honey
Where have all the people gone today
There's no need for you to be worrying about all those people
You never see those people anyway
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Re: mycelium growing out very transparent [Re: Nichrome]
#26990886 - 10/18/20 12:55 AM (3 years, 5 months ago) |
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I think it would be 1g to 99ml would be considered 1% for a final volume of 100ml ([1g=1ml]+99ml=100ml).
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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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Mycolorado
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Re: mycelium growing out very transparent [Re: maxmush]
#26991114 - 10/18/20 08:13 AM (3 years, 5 months ago) |
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Yep. Add dry ingredients and top up to target volume with water. It helps if the vessel you’re pc’ing is graduated..
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Nichrome
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Re: mycelium growing out very transparent [Re: maxmush]
#26991949 - 10/18/20 07:58 PM (3 years, 5 months ago) |
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Quote:
maxmush said:
I think it would be 1g to 99ml would be considered 1% for a final volume of 100ml ([1g=1ml]+99ml=100ml).
indeed correct.
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Where have all the people gone my honey
Where have all the people gone today
There's no need for you to be worrying about all those people
You never see those people anyway
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Clc420
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Re: mycelium growing out very transparent [Re: Nichrome]
#26995771 - 10/21/20 05:42 AM (3 years, 5 months ago) |
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So would you say to drop my LME to 5g instead of 8g with 10g agar to 500ml water to stop this thin mycelium? I just dont know why I'm getting this all of a sudden when I've always used this recipe on other cube varieties
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Nichrome
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Re: mycelium growing out very transparent [Re: Clc420]
#26996142 - 10/21/20 10:49 AM (3 years, 5 months ago) |
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Much of it is probably phenotype.
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Where have all the people gone my honey
Where have all the people gone today
There's no need for you to be worrying about all those people
You never see those people anyway
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Roundabout
On again off again
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Re: mycelium growing out very transparent [Re: Clc420]
#26997085 - 10/21/20 09:58 PM (3 years, 5 months ago) |
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Were your plates fresh? I suspect older plates may have resulted in similar for me.
I did some transfer of some paneolus cyanescens to a month or maybe 6 week old agar plate that I suspect had dried out a bit. The mycelium seems to stay below the surface of the agar rather riding on top, leading to a transparent sort of look, though it definitely leeched out the dye i used.
My assumption was that the surface was dry and not hospitable but the agar lower down still had enough moisture to be colonized. I'm paused for awhile so haven't tried making some fresh plates to transfer to.
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verum subsequentis
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Re: mycelium growing out very transparent [Re: Roundabout]
#26997168 - 10/21/20 11:29 PM (3 years, 5 months ago) |
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old plates work fine
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Clc420
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Re: mycelium growing out very transparent [Re: Roundabout]
#26997193 - 10/22/20 12:02 AM (3 years, 5 months ago) |
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Quote:
Roundabout said:
Were your plates fresh? I suspect older plates may have resulted in similar for me.
I did some transfer of some paneolus cyanescens to a month or maybe 6 week old agar plate that I suspect had dried out a bit. The mycelium seems to stay below the surface of the agar rather riding on top, leading to a transparent sort of look, though it definitely leeched out the dye i used.
My assumption was that the surface was dry and not hospitable but the agar lower down still had enough moisture to be colonized. I'm paused for awhile so haven't tried making some fresh plates to transfer to.
Naa these are fresh plates and have moisture on them man. I looked at a plate I transferred from and it was like a floppy like jelly and it was hydrated if you know what I mean.
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creekhouse
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Re: mycelium growing out very transparent [Re: Clc420]
#26998284 - 10/22/20 04:32 PM (3 years, 5 months ago) |
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I tried 8g agar, 6g LME, 5g activated carbon & 500ml water for this most recent batch of agar. Will report back on difference vs. the standard 10/10/0/500 I was trying earlier without much success.
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