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OfflineClc420
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mycelium growing out very transparent
    #26986824 - 10/15/20 12:55 PM (11 days, 15 hours ago)

I have some jedi mind fuck plates. 1 plate is 1 transfer from MS and the other is a ms inoculated plate. You can see black on the spore inoculate plate, this is just a tiny bit of the swab I cut off and placed in the plate.

I've never seen a plate grow out and be so transparent. All my other varieties have a clear leading edge while growing but nothing like this. Could it just be due the variety?



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InvisibleKmacmo
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Re: mycelium growing out very transparent [Re: Clc420]
    #26986851 - 10/15/20 01:11 PM (11 days, 15 hours ago)

What species are they?
If there is any mycelium, the Mold has taken over


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OfflineClc420
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Re: mycelium growing out very transparent [Re: Kmacmo]
    #26986853 - 10/15/20 01:12 PM (11 days, 15 hours ago)

Cubes.

Really? I dont see any mold on them just mycelium unless my eyes arent trained enough..


Edited by Clc420 (10/15/20 01:13 PM)


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OfflineAtomHeart
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Re: mycelium growing out very transparent [Re: Clc420]
    #26986897 - 10/15/20 01:39 PM (11 days, 14 hours ago)

I've had this happen before and for me it turned out being too much nutrients in the agar batch.I dialed back the malt in the next batch by 5g and everything went back to normal, but every single plate in that batch caused transparent mycelium.

It was just a simple brain fart that caused me to put in too much on that batch.


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OfflineClc420
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Re: mycelium growing out very transparent [Re: AtomHeart]
    #26986933 - 10/15/20 02:00 PM (11 days, 14 hours ago)

I have other plates going that are normal growth. That's a good point though, this batch is 8 grams lme and 10 grams agar


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Offlineverum subsequentis
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Re: mycelium growing out very transparent [Re: Clc420]
    #26987066 - 10/15/20 03:13 PM (11 days, 13 hours ago)

It could be a couple different things. Could have a contam riding along, could be the nutes, could be that some extra dimensional beings sought refuge in the agar.....

They don't look good though. All one can do is take i nice small transfer to a new plate and see what happens. I certainly would NOT advise putting those to grain though.


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OfflineClc420
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Re: mycelium growing out very transparent [Re: verum subsequentis]
    #26987073 - 10/15/20 03:16 PM (11 days, 13 hours ago)

Quote:

verum subsequentis said:
It could be a couple different things. Could have a contam riding along, could be the nutes, could be that some extra dimensional beings sought refuge in the agar.....

They don't look good though. All one can do is take i nice small transfer to a new plate and see what happens. I certainly would NOT advise putting those to grain though.





Thanks for your input. It's very weird as I've never had plates do this and I have other varieties on the fame batch of agar.

I'm gonna throw them and start new plates with the swabs.


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Offlineverum subsequentis
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Re: mycelium growing out very transparent [Re: Clc420]
    #26987121 - 10/15/20 03:51 PM (11 days, 12 hours ago)

Ok. I'd take a transfer and watch it in the name of

:havesomescience:


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OfflineClc420
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Re: mycelium growing out very transparent [Re: verum subsequentis]
    #26987140 - 10/15/20 04:04 PM (11 days, 12 hours ago)

Quote:

verum subsequentis said:
Ok. I'd take a transfer and watch it in the name of

:havesomescience:




Yeah I have nothing to loose and its better than not knowing!


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Offlineverum subsequentis
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Re: mycelium growing out very transparent [Re: Clc420]
    #26988258 - 10/16/20 01:22 PM (10 days, 15 hours ago)

indeed


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Offlinecreekhouse
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Re: mycelium growing out very transparent [Re: verum subsequentis]
    #26988292 - 10/16/20 01:43 PM (10 days, 14 hours ago)

I'm seeing something similar (except potentially even less promising) in my MSS-to-agar plates. Pretty much every plate looks like this - is this early mycelium, straight contam, or some combination of the two?



Apologies for the poor pics, they're no-pours and the condensation makes visibility tough..

I'm mainly wondering what I should change for my next batch. I'm also stuck with MSS to agar until I get some jars growing out.


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InvisibleSir Pentinite
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Re: mycelium growing out very transparent [Re: creekhouse]
    #26988327 - 10/16/20 02:11 PM (10 days, 14 hours ago)

Those shiny, slimy blobs look like bacteria.


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Re: mycelium growing out very transparent [Re: Sir Pentinite]
    #26988330 - 10/16/20 02:13 PM (10 days, 14 hours ago)

Shoot, I thought so. It's pretty much on every plate, across multiple spore syringes. What is the most likely cause in your opinion? Is it the agar recipe, or the syringes themselves?


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Offlinealaskappalachian
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Re: mycelium growing out very transparent [Re: creekhouse]
    #26988355 - 10/16/20 02:31 PM (10 days, 13 hours ago)

It's the syringes.  They are by nature host to contaminants and specifically bacteria.  As soon as the solution hits agar that bacteria finds food and goes ham.  That's why it's uber important to use the tiniest drop of spore solution going to agar from syringes.  Even going so far as to moisten a swab to apply.  Sometimes that's even futile unfortunately.  You can use brf paste (Josex has a great tek for this  because he's just a cool guy like that) to get clean myc or run some brf cakes then make prints.  The brf paste/pucks are stupid simple to make (just like prepours) and will get you squared away quickly.


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Re: mycelium growing out very transparent [Re: alaskappalachian]
    #26988469 - 10/16/20 03:30 PM (10 days, 12 hours ago)

Thanks so much, that info is all super helpful. I'm not surprised it's the syringe, I'm getting some serious contam in my grain jars that I'm knocking up alongside my agar plates and the contam always seems to be directly where I squirted the spore solution.

With my most recent batch of agar plates, I did individually sterilize q tips and tried to do 3-4 drops of MSS on the qtip itself then rubbed the agar. Hopefully that will help.

I used Josex' no pour agar tek and loved everything about it so I'll definitely check out his BRF paste tek (which I hadn't heard of). Appreciate you sharing your knowledge.


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Offlinealaskappalachian
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Re: mycelium growing out very transparent [Re: creekhouse]
    #26988484 - 10/16/20 03:39 PM (10 days, 12 hours ago)

:saulgoodman:  And I don't remember if his tek specifies, but don't sweat the amount of brf in the pucks.  You're just heating up brf and water until it barely is pourable, pouring it quickly,  and tapping it gently to level off.  As long as it's thick enough to set up pretty firm and thin enough to poooouuuur,  you're gold, my friend.


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OfflineRogerRabbitM
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Re: mycelium growing out very transparent [Re: Clc420]
    #26988516 - 10/16/20 03:55 PM (10 days, 12 hours ago)

Quote:

Clc420 said:







I've seen this before.  As said above, it could be your agar is too nutrient dense.  Make several transfers to new plates a with a bit less nutrients and see how it progresses.  I see no bacteria or obvious mold.
RR


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Offlineverum subsequentis
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Re: mycelium growing out very transparent [Re: RogerRabbit]
    #26989477 - 10/17/20 04:19 AM (10 days, 5 minutes ago)

:whathesaid:

hi roger.


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OfflineClc420
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Re: mycelium growing out very transparent [Re: RogerRabbit]
    #26989824 - 10/17/20 11:49 AM (9 days, 16 hours ago)

Quote:

RogerRabbit said:
Quote:

Clc420 said:







I've seen this before.  As said above, it could be your agar is too nutrient dense.  Make several transfers to new plates a with a bit less nutrients and see how it progresses.  I see no bacteria or obvious mold.
RR





I'm using 8 grams LME and I have other cultures growing out fine. Would you say 8 grams lme is too much ? I'm sure I've seen you reccomend that a few times.


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InvisibleMycolorado
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Re: mycelium growing out very transparent [Re: Clc420]
    #26989884 - 10/17/20 12:39 PM (9 days, 15 hours ago)

8 grams to what volume of media?  Percent is the needed info. 2% nutes is pretty standard.


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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Amazon ½ Pint Jars, Agar, Brown Rice Flour

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