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Offlinepitriot
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Lions Mane agar?
    #26986125 - 10/14/20 10:14 PM (3 years, 3 months ago)

I have these two agar dishes that I injected with Lions Mane culture 2 weeks ago.
Do they look good?
If so what is the best way to inoculate grain with this?





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OfflineQuadman
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Re: Lions Mane agar? [Re: pitriot]
    #26986139 - 10/14/20 10:25 PM (3 years, 3 months ago)

First pic is out of focus, can't make out details.

The second pic , I'm not sure is lions mane. Lions mane ( erinaceus) makes clumps not spikes. To me that could be oyster trying to fruit? :shrug:


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Edited by Quadman (10/14/20 10:34 PM)


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Offlinepitriot
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Re: Lions Mane agar? [Re: Quadman]
    #26986151 - 10/14/20 10:37 PM (3 years, 3 months ago)

It is from a syringe that I purchased that says Lions Mane. I have done Pink Oyster before and it does have some similarity to this. In he morning I will upload a better picture.


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InvisibleBsdgaou

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Re: Lions Mane agar? [Re: pitriot]
    #26986248 - 10/14/20 11:58 PM (3 years, 3 months ago)

First picture I am sure it's LM, I have it the same.
Second pic I am with Quadman, looks like oysters want to pin.


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OfflineForresterM
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Re: Lions Mane agar? [Re: Bsdgaou]
    #26986365 - 10/15/20 02:24 AM (3 years, 3 months ago)

What they said.  That's not erinaceous in the 2nd pic.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Lions Mane agar? [Re: Forrester]
    #26986519 - 10/15/20 06:54 AM (3 years, 3 months ago)

Are both jars the same syringe?


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Offlinepitriot
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Re: Lions Mane agar? [Re: Quadman]
    #26986829 - 10/15/20 10:59 AM (3 years, 3 months ago)

Both are from same syringe injected at the same time.

Here is a better picture of the one that was blurry. (Still not great pic but best I can seem to get.)



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OfflineQuadman
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Re: Lions Mane agar? [Re: pitriot]
    #26986895 - 10/15/20 11:38 AM (3 years, 3 months ago)

Never have I seen lions mane act like that. I'm not sure what you have. It seems to want to fruit though.:lol:


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Offlinesporecap
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Re: Lions Mane agar? [Re: Quadman]
    #26987115 - 10/15/20 01:48 PM (3 years, 3 months ago)

I think it looks like Lion's Mane that's desperately reaching for air.


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Re: Lions Mane agar? [Re: sporecap]
    #26987211 - 10/15/20 02:38 PM (3 years, 3 months ago)

Hmmm... I guess it's been a while but I didn't remember lion's mane doing that!  :shrug:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinepitriot
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Re: Lions Mane agar? [Re: sporecap]
    #26987270 - 10/15/20 03:09 PM (3 years, 3 months ago)

I was told that petri dishes do not need AE and even so this has a nice size hole covered with micro-pore tape.


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Offlinepitriot
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Re: Lions Mane agar? [Re: pitriot]
    #26987271 - 10/15/20 03:10 PM (3 years, 3 months ago)

What is the best way to inoculate grain with this?


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Re: Lions Mane agar? [Re: pitriot]
    #26987410 - 10/15/20 05:01 PM (3 years, 3 months ago)

I would take a wedge from where there's the least amount of white.  You want mycelium, not fruit.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinepitriot
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Re: Lions Mane agar? [Re: Forrester]
    #26987573 - 10/15/20 07:32 PM (3 years, 3 months ago)

Why is the mycelium preferred over fruit?

Would you use the mycelium to make liquid culture or put it strait to grain?


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Re: Lions Mane agar? [Re: pitriot]
    #26987887 - 10/16/20 01:56 AM (3 years, 3 months ago)

Not that a fruit won't work, but we don't usually inoculate grain jars with them.  Mycelium is preferred because it's still in "colonizing" mode and that's what you want it to do, colonize the grains. 

Also, when there's fruits involved on agar, there's always the chance of a spore or two being present.  In which case your growth you get after a transfer could be from spores, which would mess up your culture (by being a different culture).  You generally want to avoid that if you like the culture you have.

It's up to you if you want to make LC or not.  If I'm going to grow a lot of something I prefer LC so I can inoculate grain in open air with a syringe.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinepitriot
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Re: Lions Mane agar? [Re: Forrester]
    #26988032 - 10/16/20 07:38 AM (3 years, 3 months ago)

Why is inoculating with LC in open air ok but not agar or spores?


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OfflineQuadman
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Re: Lions Mane agar? [Re: pitriot]
    #26988378 - 10/16/20 12:38 PM (3 years, 3 months ago)

LC would have to be taken up sterile in a syringe. Once in a syringe you can inject into anything provided you follow sterile procedure  ( alcohol wipe injection sites, flame needle,  etc.)

You have to open and physically remove the agar with scalpel. Then physically open your grain or whatever. This requires flow hood or still airbox and proper techniques.


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Edited by Quadman (10/16/20 12:43 PM)


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Offlinesporecap
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Re: Lions Mane agar? [Re: Forrester]
    #26988565 - 10/16/20 02:22 PM (3 years, 3 months ago)

Quote:

Forrester said:
Hmmm... I guess it's been a while but I didn't remember lion's mane doing that!  :shrug:



I digged out an old plate of Lion's Mane from the back of my fridge, and while it's not exactly doing the same thing, I think it's reasonably close. :smile: I think OP is fine.


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OfflineQuadman
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Re: Lions Mane agar? [Re: sporecap]
    #26988704 - 10/16/20 03:35 PM (3 years, 3 months ago)

Quote:

sporecap said:
Quote:

Forrester said:
Hmmm... I guess it's been a while but I didn't remember lion's mane doing that!  :shrug:



I digged out an old plate of Lion's Mane from the back of my fridge, and while it's not exactly doing the same thing, I think it's reasonably close. :smile: I think OP is fine.




Take note of the rest of your plate compared to OP. His is much thicker mycelium. As I said from the beginning  it is possible,  but doubtful. Fruit it will tell for sure.:lol:


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OfflineForresterM
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Re: Lions Mane agar? [Re: Quadman]
    #26988852 - 10/16/20 04:52 PM (3 years, 3 months ago)

Quote:

Quadman said:
Take note of the rest of your plate compared to OP. His is much thicker mycelium. As I said from the beginning  it is possible,  but doubtful. Fruit it will tell for sure.:lol:




I'm still with you here, interested to see if we find out!


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinepitriot
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Re: Lions Mane agar? [Re: Forrester]
    #26990747 - 10/17/20 09:52 PM (3 years, 3 months ago)

Since I still have most (95%) of the syringe I will inject it to grain and see how it behaves. I don't like putting agar to grain as it takes too long and fails too often for me.


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