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fraki58
Mycology Learner


Registered: 11/20/19
Posts: 245
Last seen: 5 days, 9 hours
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Come take a look at this spore plates! Few questions
#26985560 - 10/14/20 04:59 PM (3 years, 3 months ago) |
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These are my first spore plates that thrived, i omitted some of them cause they all have similar growth. My inquiries are:
-The spores are B+ Akm and GT, they both show really different myc, GT is whiter, like more cottonier while the B+ is not. Other varieties show different kinds of myc as well? like B+ is always like that or the same variety can show differences in terms of growth? Not talking about if it's rhizo or tomentose
-Do any of the pics shown here show any contamination? I'm trying to learn common contaminants appearing on my own plates so i'll know in the future what am looking at.
-The B+ colonized this whole plate a few days ago and now it's looking like the myc is forming something rising from the agar, are these 'knots'? Will they turn into fruiting bodies in the next days? If not, what are these? On the pic of the plate from the side you can see it pretty well. Not one GT plate is showing this conditions, but all the B+ are, to some degree, this be the plate with far more advanced growth than the others.
   
-On the GT plates some tiny droplets started to form. I've seen something like this on posts regarding 'surface conditions' on tubs and such, so, is this what this afored mentioned conditions are? Only they appeared on agar?
   
I know some plates were poured badly, rooms for improvement! my most recent plates look nothing like this luckily haha! I appreciate any input anyone can give me!
-------------------- If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Come take a look at this spore plates! Few questions [Re: fraki58]
#26986262 - 10/15/20 12:07 AM (3 years, 3 months ago) |
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Are these germination plates or transfer plates? I'm assuming the former and I know they can look kinda crazy sometimes but that looks very much like some kind of mold and not cube myc. Crazy unorganized growth pattern. You can find pics of spore syringe inoculated plates on the forums and compare.
What was your process like? What do your other plates look like?
I'm fairly new to this so perhaps someone else will chime in but those plates look like bad news.
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fraki58
Mycology Learner


Registered: 11/20/19
Posts: 245
Last seen: 5 days, 9 hours
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Re: Come take a look at this spore plates! Few questions [Re: Lenz]
#26986482 - 10/15/20 05:52 AM (3 years, 3 months ago) |
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Hi Lenz! Yes they are germination plates, they were inocced from the prints using a loop doing the streak, i did like 8-9 zigzags on each one so im guessing thats why they growed unorganized like that? All of them look like that, two of them showed contam but the myc looks similar. I already did a couple transfers from 2 of the germ plates and more transfers out of those and they are comming pretty good so far. In a few days I'll be doing A2G if they look healthy, if not transfer! Thanks for the response!
-------------------- If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best
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Pearl
Stranger


Registered: 05/28/20
Posts: 261
Last seen: 1 year, 3 months
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Re: Come take a look at this spore plates! Few questions [Re: fraki58] 1
#26987300 - 10/15/20 03:32 PM (3 years, 3 months ago) |
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Are those missing chunks taken out your transfers? If so they don't need to be so massive. 
Growth looks normal for that kind of streak job - I think everyone just goes ham at first as there's no experience with working with unseen things.
I'd see how your transfers do and likely transfer a few more after that. Don't rush to grain unless you want what would still be a bit of a crap shoot (as one transfer likely won't be enough to verify it's clean).
Also, as you're going from print to agar, you'll going for the most robust grower, but do keep in mind that that doesn't mean too much in how it fruits. Don't assume a pretty rhiso agar dish means you'll be harvesting a nice canopy.
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fraki58
Mycology Learner


Registered: 11/20/19
Posts: 245
Last seen: 5 days, 9 hours
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Re: Come take a look at this spore plates! Few questions [Re: Pearl]
#26987325 - 10/15/20 03:44 PM (3 years, 3 months ago) |
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Hey Pearl, the missing chunks is just some bad pouring lol, didn't poured enough in them haha. Great! I'm already on my second transfers, taken the 09/10, gonna wait how those go but i think they are pretty good, will post them later on to know if they are as good as i think to go grain with them, care to see them? Also, do you know what the myc rising from the agar is on the b plates? I'm guessing knots forming but not entirely sure. Anyways thanks for responding!
-------------------- If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best
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