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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Day 10 Colonization of Substrate [Re: Greens21]
    #26985447 - 10/14/20 04:02 PM (3 years, 4 months ago)

Well it's failed time and time again for people that aren't you... So.... Do we go on one person's experience, or the thousands of grows that have been posted here? Which would you rely on? A single personal anecdote or repeated documentation from multiple sources?


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OfflineGreens21
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Re: Day 10 Colonization of Substrate [Re: Munchauzen]
    #26985463 - 10/14/20 04:09 PM (3 years, 4 months ago)

It’s failed time and again for people that store their jars the same way they would if they had inoculated with agar, which isn’t surprising that it doesn’t work, so...

Browse very old posts on this site. There was a time that almost no one was using agar. Do you think they all had “very high” contam rates? No, agar just proved to be the superior method and then as people started trying to combine syringe teks with agar teks they started having massive problems. Syringe teks exist and agar teks exist. The agar teks probably result in 3-4 day faster colonization in subsequent grows if your plan is to continue indefinitely, but not everybody plans to continue indefinitely, or wants to spend the extra 80-100 bucks on top of everything else they purchased to do their first grow. And it’s silly to pretend like syringe teks just fail at massive rates when the real reason they aren’t recommended anymore is because they take slightly longer than agar and require slightly more attention to detail during germination and colonization.


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I've been trying to justify you

In the end I will just defy you







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OfflineOne of Us
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Re: Day 10 Colonization of Substrate [Re: Greens21]
    #26985465 - 10/14/20 04:09 PM (3 years, 4 months ago)

Who said you were being logical?

Ok, I'll stop.

But claiming over 150 jars without contam without seeing a contam is not really possible. I don't think anyone here has never seen a contam (besides those who have never grown). But you could be the one, Neo


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OfflineGreens21
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Re: Day 10 Colonization of Substrate [Re: One of Us]
    #26985479 - 10/14/20 04:17 PM (3 years, 4 months ago)

The only time I ever saw a contam was with my very first BRF grow using a tek I got off some “shroom wizard” site 15 years ago.

Once I found this site I used a tek that has since been deleted to make self-healing inoculation port jars using WBS. Flame-sterilize the needle and stick into an alcohol-soaked makeup sponge (yes), and inoculate without the needle or jar’s contents ever coming into contact with unsterile air.

The jars are stored at incubation temperatures in complete darkness, which both promotes mycelial growth and inhibits bacterial and mold growth.

My last grow was literally done in the shower of a 30-year old RV while I was traveling around the country. Several people using the toilet a couple feet away from the grow the entire time, from inoculation to harvest, with zero contaminates and thousands of dollars made at festivals.

You think you know what you’re accusing me of, but you don’t.


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I've been trying to justify you

In the end I will just defy you







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OfflineBlazer Man
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Re: Day 10 Colonization of Substrate [Re: Greens21]
    #26985495 - 10/14/20 04:26 PM (3 years, 4 months ago)

There is no need to argue over my project or who has done what. It's a hobby for crying out loud. Greens21, I messaged you. Holler when you get a chance.


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OfflineOne of Us
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
    #26985506 - 10/14/20 04:32 PM (3 years, 4 months ago)

I agree, this isn't the place.

Greens, you should start a new thread where you can describe this tek of yours and change the world. No one will ever see contams again. Don't be greedy with your trade secrets.


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OfflineGreens21
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Re: Day 10 Colonization of Substrate [Re: One of Us]
    #26985518 - 10/14/20 04:39 PM (3 years, 4 months ago)

I had actually been thinking about creating a tek, but our other friend in this thread made it clear that teks are “policed.” Since it’s largely derived from a tek that’s been deleted from the site since I stopped visiting, I assume it doesn’t fit the narrative of “only agar” and will get removed.

If there’s some assurance this wouldn’t happen and be a complete waste of my time, I’d be happy to create a tek and even a grow log using it.

Also, this is only for MS syringe inoculation. While it may lead to reduced/no contaminates, MS won’t be the preferred method of growing for most growers. That doesn’t mean it doesn’t work the way I’ve been saying, though.


--------------------
I've been trying to justify you

In the end I will just defy you







Edited by Greens21 (10/14/20 04:54 PM)


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InvisibleMunchauzen
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Re: Day 10 Colonization of Substrate [Re: Greens21]
    #26985693 - 10/14/20 05:58 PM (3 years, 4 months ago)

Quote:

Blazer Man said:
There is no need to argue over my project or who has done what. It's a hobby for crying out loud. Greens21, I messaged you. Holler when you get a chance.



Part of the culture of shroomery is advocating for the best methods with the least amount of issues. Spores to grains falls pretty far outside the circle of accepted methods. As you are having issues with contaminates, I'm just pointing out one of the major vectors that could be a cause for this.

Also, don't ever cut a chunk of mold out of a substrate. You're making it 10x worse by doing so. There are now fragmented mold particles colonizing all across the surface of your tub. In a few days, there will be much more mold than there was before you cut it out. The proper way to deal with mold is to cover it a generous amount of salt, cover the salt with paper towels to soak up the water thats going to ooze from it, and harvest what fruit you have ASAP, then pitch the container. There is no removing of mold, only removing of moldy tubs.

Quote:

Greens21 said:
I had actually been thinking about creating a tek, but our other friend in this thread made it clear that teks are “policed.” Since it’s largely derived from a tek that’s been deleted from the site since I stopped visiting, I assume it doesn’t fit the narrative of “only agar” and will get removed.

If there’s some assurance this wouldn’t happen and be a complete waste of my time, I’d be happy to create a tek and even a grow log using it.

Also, this is only for MS syringe inoculation. While it may lead to reduced/no contaminates, MS won’t be the preferred method of growing for most growers. That doesn’t mean it doesn’t work the way I’ve been saying, though.




some confidence you have in your method. post it up for others to try it out, or just drop it already. teks don't get removed for being subpar. they only get removed for breaking the forum rules.


Edited by Munchauzen (10/14/20 07:03 PM)


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OfflineOne of Us
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Re: Day 10 Colonization of Substrate [Re: Greens21]
    #26985715 - 10/14/20 06:10 PM (3 years, 4 months ago)

I'm sure I'm not the only one interested to hear your method, so there is that.  You definitely sound confident.

I too first started cultivating around thirteen years ago, but I took a long break and was shocked to see all the changes, and new discoveries.

I'll tell you what, if you write a tek, I will give it a try and share my results. Its gotta be reliably reproducible, right?

I assume you make your own syringes, right? That would be expensive if you didn't. Much more so than the cost of starting agar (about $50, maybe, assuming you have a PC already for your grains). After perfecting sterile technique you'll have limitless spawn.

So many more questions, but I'll save that for your own thread.

XXX'XXXXXXXXXXXXXXXXXXXXXX

to the OP, I am super sorry, I didn't realize no one really answered  your questions. 

It is pretty hard to see, but if you have already cut mold out, than you likely have contams throughout.  Since this is your first grow, if you really want to see fruits without starting over you can just leave it be. Mold will probably show up again, but you might get enough for a few trips before it completely takes over.  The shrooms are still fine to eat unless there is mold growing directly on them.

It could be possible you get nothing, but it doesn't hurt to try. Watch it and let it be a learning experience. You'll definitely know when you NEED to throw it out.

Some things to point out: once you have healthy, fully colonized grain, you no longer have to worry much about contamination. Coir is very contam resistant. So the heating of the spoon and the filter on the fan are uneeded. Take a look at a specific tek if you want to really be successful. Modern teks work flawlessly without any need for any major modification done on the fly, like your fan setup. Make sure any information you use is recent and not from a 20 year old thread.

Good luck and welcome to The Shroomery.


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OfflineBlazer Man
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Re: Day 10 Colonization of Substrate [Re: One of Us]
    #26987405 - 10/15/20 04:57 PM (3 years, 3 months ago)

Update for those who are interested. My tub died. Green mold. I am tossing and starting over. Thanks to those who helped.


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Offlinesmalltalk_canceled
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Re: Day 10 Colonization of Substrate [Re: Blazer Man] * 1
    #26987488 - 10/15/20 06:26 PM (3 years, 3 months ago)

RIP




Epitaph

Here lies a brave soldier.

He was born when Blazer man sprinkled spores on grains
in 2020.

When the mold-mycelium wars broke out,
blazer man's tub enlisted in the expeditionary force
for the mycelium minute men, even though
he was only an infant, less than a year old.


  Mycelium can fight, but it cant always win.
Blaze man's tub died selflessly in the mold-mycelia wars of 2020.
Childless, and unmarried.
  Defending a half colonized tub
  from the hordes of Mong Trichula,
  he fell on his post.

He will not continue in the great outdors,
like so many spent tubs,
but go straight to heaven.
Where the RH is always 99%.

His dying wish was for blaze man to learn agar.

Sleep now soldier, we'll take it from here.


--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (10/15/20 06:38 PM)


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