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Blazer Man
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Day 10 Colonization of Substrate
#26984999 - 10/14/20 12:04 PM (3 years, 4 months ago) |
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This is my FIRST POST AND FIRST TUB! I am on day ten since I moved my GS to my tub. Temps range between 66F and 69F. My RH says 99.7 on my Ink Bird humidity reader. I have natural light. I am noticing a little yellowing, but I think it is piss. I did have one mold spot so I sterilized a spoon, my hands and area with alcohol and dug it out (bottom center of first pic). Does this batch look normal and should I now case with coco coir or give it a few days? I am trying to be as patient as I can, but my mycelium does not look like it is trying to pin. Yesterday I started introduced a little FAE via a computer fan mounted on my bin (the air runs through filters to limit contam). Give me your feedback and or REASSURANCE please. I have done so much research and I really want to be able to master this science! Thank you all!

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tryptkaloids
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985030 - 10/14/20 12:18 PM (3 years, 4 months ago) |
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May be the lighting but it looks pink
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
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Re: Day 10 Colonization of Substrate [Re: tryptkaloids]
#26985035 - 10/14/20 12:21 PM (3 years, 4 months ago) |
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Computer fan and hygrometer won't help you.
Myc doesn't piss, and alcohol doesn't sterilize, it sanitizes
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Blazer Man
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Re: Day 10 Colonization of Substrate [Re: tryptkaloids]
#26985046 - 10/14/20 12:29 PM (3 years, 4 months ago) |
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Thanks for the reply. I know experienced people hate answering these questions. It is definitely not pink in person. Actually, it is mostly white with a little bit of yellowing. From what I have read, my temps are not perfect, but I don't have a choice since they are in my basement so I know that will slow down growth. 68F is the best I can do down there. I seem to have good exudate and such. Maybe I am just looking to hear someone say "it looks ok, keep going" (hopefully they mean it too ha).
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Justweed
Bobby's short shorts



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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985062 - 10/14/20 12:37 PM (3 years, 4 months ago) |
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You've already encountered contaminant, the yellow piss is a sign that you most likely have more. Don't case that, it'll allow the contaminant to breed more. Set aside outside of your grow area if you don't want to throw it out.
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  Keep 'em high and tight guys....
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footpath
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985070 - 10/14/20 12:40 PM (3 years, 4 months ago) |
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It's hard to say for sure because of the image quality and the water droplets all over the surface. From my eyes, as well, there are some yellow and red hues going on. If I had to guess, I'd say it looks pretty marshmallowy, which usually is indicative of a bacterial contamination. You can still pull some fruits with a bacterial contamination, so, I'd say let it go - see if it fruits, but don't expect much. 10 days is still on the early side to be expecting pins. Also, 68˚F is not too low for them to perform well. Also also, ease up on the misting.
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Blazer Man
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Re: Day 10 Colonization of Substrate [Re: footpath]
#26985094 - 10/14/20 12:54 PM (3 years, 4 months ago) |
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I turned off my LED strip and took another couple of pictures. I used spores from a popular vendor, a grain bag from another popular vendor. I sanitized and cleaned everything and did not open my tub until 2 days ago. If I have contams it would be devastating because I did this so carefully not to get contams. Anyway, I think these pics are better.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985149 - 10/14/20 01:25 PM (3 years, 4 months ago) |
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Quote:
Blazer Man said: I turned off my LED strip and took another couple of pictures. I used spores from a popular vendor, a grain bag from another popular vendor. I sanitized and cleaned everything and did not open my tub until 2 days ago. If I have contams it would be devastating because I did this so carefully not to get contams. Anyway, I think these pics are better.

Shooting spores on to grains has a high chance for contamination. Doesn't matter who made the syringe.
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Greens21
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Re: Day 10 Colonization of Substrate [Re: Munchauzen]
#26985213 - 10/14/20 01:59 PM (3 years, 4 months ago) |
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Quote:
Munchauzen said:
Shooting spores on to grains has a high chance for contamination. Doesn't matter who made the syringe.
This is super interesting to me, because I haven’t been active on this site for 13 years, and back then, inoculating grain with a syringe was pretty much the standard, but I see people saying this pretty much every day here now.
I understand that knowledge changes as the years go on, but I continued to do grows every year or so without using this site over those 13 years, and seeing that I continued to use the methods I learned back then, I’ve probably inoculated 150 or so grain jars from syringe and have had zero percent contamination. Never lost a single jar or tub.
Maybe it’s that sterile methods can be laxxed a bit if you’re using agar and that makes life easier, but considering my personal experience I find it hard to believe that inoculating grains with spores has a “very high” contam rate as long as it’s done correctly.
-------------------- I've been trying to justify you In the end I will just defy you
Edited by Greens21 (10/14/20 02:10 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Day 10 Colonization of Substrate [Re: Greens21]
#26985223 - 10/14/20 02:07 PM (3 years, 4 months ago) |
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Consider yourself lucky. Facts are spores are never aseptic.
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Greens21
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Re: Day 10 Colonization of Substrate [Re: Munchauzen]
#26985238 - 10/14/20 02:12 PM (3 years, 4 months ago) |
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Sure, but you can create conditions that are good for spores and bad for contaminates.
Maybe I am just lucky, obviously even with my 150+ inocced jars you have way more experience than I do, but 0 out of 150 still kind of seems to be beyond just beating the odds.
-------------------- I've been trying to justify you In the end I will just defy you
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Munchauzen


Registered: 06/22/11
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Re: Day 10 Colonization of Substrate [Re: Greens21] 1
#26985306 - 10/14/20 02:45 PM (3 years, 4 months ago) |
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Not really a topic up for discussion TBH. If you wanna shoot spores on grains, great. But it's not a tek we recommend here for multiple reasons and you'll continue to get resistance from other members here if you keep suggesting it. We police our community for the best methods and practices to ensure success, not the methods that sorta work sometimes for lucky people. Countless grows, probably in the thousands by now, have shown conclusively that spores on grains is a significant contamination vector.
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Greens21
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Re: Day 10 Colonization of Substrate [Re: Munchauzen]
#26985331 - 10/14/20 02:55 PM (3 years, 4 months ago) |
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Sure, now that everyone recommends no incubation and storing jars in ambient light, I’m sure that spore syringes don’t work well in those situations where agar does, but the reason they were recommended back then is because they worked for inoculations from syringe or print which is how 95% of the community was doing it.
Im not arguing that agar isn’t better and safer, but there are various reasons that people can’t afford to/don’t want to commit to agar, and if there are methods of syringe inoculation that do work well, they should be available to people who need them rather than stifled because there are more expensive and more time consuming methods that have similar success rates with less attention to detail.
It wasn’t my intention to derail the thread, I won’t have anything to say beyond this, I just figured it’s worth it for people to know that people made monster grows with low contamination rates before agar was widespread and they still do, it just has to be done right.
Edited by Greens21 (10/14/20 03:20 PM)
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Blazer Man
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Re: Day 10 Colonization of Substrate [Re: Greens21]
#26985398 - 10/14/20 03:37 PM (3 years, 4 months ago) |
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SOOOO now that we have gotten off topic, HAHA, is this a general consensus that my batch is contaminated? Dang nabbit...
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Greens21
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985405 - 10/14/20 03:41 PM (3 years, 4 months ago) |
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Given that I have zero experience with contams, I can’t help you, other than to say that it doesn’t really look like any healthy grow I’ve ever had.
-------------------- I've been trying to justify you In the end I will just defy you
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Blazer Man
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985412 - 10/14/20 03:45 PM (3 years, 4 months ago) |
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Also, I am just starting so there is not any other method of obtaining spores other than syringes. Unless I am wrong?
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985422 - 10/14/20 03:51 PM (3 years, 4 months ago) |
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Prints & swabs.
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Greens21
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Re: Day 10 Colonization of Substrate [Re: Blazer Man]
#26985429 - 10/14/20 03:55 PM (3 years, 4 months ago) |
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The recommendation is that you use spores to inoculate agar, then you can use agar to isolate mycelium away from contaminates, then use that mycelium to inoculate grain. It’s likely superior to any method of direct inoculation, but if you need a tek that works very well without the extra time and expense of agar and petri dishes and still air boxes etc just PM me and I’ll explain how to successfully inoculate from syringe, because apparently no one else here will do it and will instead call you stupid for not wanting to buy a bunch of extra stuff
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One of Us
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Re: Day 10 Colonization of Substrate [Re: Greens21]
#26985431 - 10/14/20 03:55 PM (3 years, 4 months ago) |
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Quote:
Greens21 said: Given that I have zero experience with contams, I can’t help you, other than to say that it doesn’t really look like any healthy grow I’ve ever had.
You ain't gotta lie to kick it. I still think you're cool no matter how many of your syringe to grains failed
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Greens21
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Re: Day 10 Colonization of Substrate [Re: One of Us]
#26985432 - 10/14/20 03:57 PM (3 years, 4 months ago) |
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Right, because it logically makes sense that I’d be here defending a method that failed repeatedly over 13 years
-------------------- I've been trying to justify you In the end I will just defy you
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