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bballadict540
Stranger

Registered: 11/07/19
Posts: 1
Last seen: 3 years, 3 months
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Please help identify what went wrong! Thank you
#26984827 - 10/14/20 10:25 AM (3 years, 3 months ago) |
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Hi there! So I need some help identifying what exactly is growing in these jars and if anyone has experienced this before. Black bits are coffee grounds.
Inoculated jars using liquid culture and thought I kept process pretty sterile but all jars seem to have developed differently than I am used to. Within 3 days jars looked like this... need help verifying that this is in fact contamination as there are white fine threads (resembling very thin mycelium) present. However, opened one and it is dusty.
Tossed jars but looking to not repeat.
Should I start over and try again using same liquid culture?
Any help is appreciated. Mush love y’all.

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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Please help identify what went wrong! Thank you [Re: bballadict540]
#26984841 - 10/14/20 10:29 AM (3 years, 3 months ago) |
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Definitely toss the culture. It's garbage. You are growing straight mold. What do you mean "pretty sterile"? Most things in life aren't absolute but sterile is sterile. There can be no "pretty sterile" when it comes to innoculating grain.
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LoadedFish
Psilocybin Enthusiast


Registered: 07/12/20
Posts: 107
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Re: Please help identify what went wrong! Thank you [Re: bballadict540]
#26989432 - 10/17/20 01:27 AM (3 years, 3 months ago) |
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Yeah those are fucked. I don’t have any working experience with LC but maybe you should consider agar instead? Seems easier to identify contams. Best of luck
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MycLife
(っ ❛ ͜ʖ ❛ )っ🎔



Registered: 10/16/20
Posts: 28
Loc: USA
Last seen: 9 months, 3 days
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Re: Please help identify what went wrong! Thank you [Re: LoadedFish]
#26989443 - 10/17/20 01:40 AM (3 years, 3 months ago) |
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To be sure, take some of that liquid culture and put it to agar in a still air box or in front of a flow hood. If it contams on your agar too, toss that LC.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Please help identify what went wrong! Thank you [Re: MycLife]
#26989461 - 10/17/20 02:02 AM (3 years, 3 months ago) |
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my money is on it tamming out.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 19 hours
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Quote:
verum subsequentis said: Definitely toss the culture. It's garbage. You are growing straight mold. What do you mean "pretty sterile"? Most things in life aren't absolute but sterile is sterile. There can be no "pretty sterile" when it comes to innoculating grain.

Also, leave coffee grounds out of your grain spawn. And everywhere else.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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