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Offlinepoopforballs
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Registered: 09/15/19
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Can I use tissue sample to LC?
    #26983339 - 10/13/20 12:59 PM (3 years, 3 months ago)

is it bad to put tissue in LC or should it be grown on agar first


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Can I use tissue sample to LC? [Re: poopforballs]
    #26983365 - 10/13/20 01:13 PM (3 years, 3 months ago)

You could do it but I would STRONGLY recommend agar first. I've never put tissue to LC but it could work in theory. Problem is contams on the first tissue sample plate are far to common. It'll only take a week or so to put it on agar and verify its cleanliness. Then you can go to LC with confidence.


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Offlinepoopforballs
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Re: Can I use tissue sample to LC? [Re: verum subsequentis]
    #26983478 - 10/13/20 02:29 PM (3 years, 3 months ago)

using water from oats.

same thing as if u where to put it on agar.
could she also cut a growing strip of mycelium ( im not sure what to call it ) from grain and use that. was wondering about finding the longest mycelium strip on top of the grain and using that.


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Offlineverum subsequentis
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Re: Can I use tissue sample to LC? [Re: poopforballs]
    #26983482 - 10/13/20 02:31 PM (3 years, 3 months ago)

just take a small bit of myc from the best looking area. Use a flame sterilized scalpel.


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