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OfflineSenior-Meow
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Inoculated jars with agar - what's going on overthere??
    #26982747 - 10/13/20 03:36 AM (3 years, 4 months ago)

Hello people! I inoculated 2 jars with agar 4 days ago. Today as I woke up I've noticed some liquid been released from some grains.

Jar 1



jar2



I been dealing with bacteria for a while, and I just want to understand if this liquid is normal, or an early sign for bacterial contamination?
The temperature in the room are between 70-75f.


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OfflineKmacmo
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Senior-Meow]
    #26982832 - 10/13/20 06:57 AM (3 years, 4 months ago)

Really hard to tell with those pictures


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OfflineSenior-Meow
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Kmacmo]
    #26982849 - 10/13/20 07:28 AM (3 years, 4 months ago)

ok, I try again, need to improve my pic skills.. thanks for the comment
I hope the next pictures are better.

The liquid btw only comes out from one grain in each jar. The grain looks more brown than the rest. I'm wonder if it's just a grain pressed over the glass or my worst nightmare - bacteria :sad:
It doesn't look slimy so far and didn't spread to other grains since I first saw it..


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OfflineArthurFungarelli
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Senior-Meow]
    #26982858 - 10/13/20 07:37 AM (3 years, 4 months ago)

Based on your first pic, I was going to say it looks like grains pressed to the glass with just a little extra moisture right there.  After seeing the second set of pics, I'm pretty confident that's what it is.


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OfflineSenior-Meow
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Re: Inoculated jars with agar - what's going on overthere?? [Re: ArthurFungarelli]
    #26982920 - 10/13/20 08:21 AM (3 years, 4 months ago)

Thank you so much!!
I hope you're right. I had such a bad run with bacteria:/


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InvisibleDarkshadow
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Re: Inoculated jars with agar - what's going on overthere?? [Re: ArthurFungarelli]
    #26982927 - 10/13/20 08:24 AM (3 years, 4 months ago)

A little moisture is fine, you just want to watch out for a puddle forming in the bottom of the jars for it to be an issue (over hydrated grain). Next time you go agar to grain you can instead of using the whole puck of colonized agar, cut it into pieces so you will have more inoculating points instead of the one that you currently have placed in the jar.


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OfflineSenior-Meow
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Darkshadow]
    #26982948 - 10/13/20 08:38 AM (3 years, 4 months ago)

I usually cut my agar in half/quarters.. but I had mold on that specific plate and decided to make less cuts as possible.
I will look out for water pooling tho.. Thanks.


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OfflinePearl
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Senior-Meow]
    #26983110 - 10/13/20 10:16 AM (3 years, 4 months ago)

Why are you putting moldy plates to grain...?

That's like the opposite of what you should be using agar for...


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InvisibleBecky G. Spot
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Senior-Meow]
    #26983121 - 10/13/20 10:24 AM (3 years, 4 months ago)

As for the moisture, looks normal for jars to me. :thumbup:

That is A LOT of agar though. You really up the contamination chances when you go big like that. Especially if you are still working on learning things / perfecting sterile technique etc.

Quote:

Senior-Meow said:
I usually cut my agar in half/quarters.. but I had mold on that specific plate and decided to make less cuts as possible.
I will look out for water pooling tho.. Thanks.




Case in point here. You should transfer to new plates until you rid yourself of mold problems, not throw the whole moldy dish in there thinking that more cuts is what will give you problems... More cuts is great as long as those "more cuts" is going into more transfers to get things more and more cleaned up.

XOXOX


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OfflineSenior-Meow
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Re: Inoculated jars with agar - what's going on overthere?? [Re: Pearl]
    #26983129 - 10/13/20 10:31 AM (3 years, 4 months ago)

It was a small selatlite NEAR my culture, not on it. I didn't transfered the selatlite.
I also did the transfer while the mold selatlite was extremely small - wanted to see if the contamination passes to the jar or not..
4 days later and no sign for that mold - so far.
Anyway that was just a test, the second jar i inoculated was from a plate with no visible contam


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