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OfflineMycobro420
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Agar with clear ring around the mycilum
    #26981611 - 10/12/20 02:07 PM (3 years, 3 months ago)

So iv been doing agar work for awhile now at first I was having great results.i use the same tek with the same medium. But recently my agar has been coming out strange. It is slow growing and have the almost clear ring around the edges of the myc. To my untrained eye it looks like bacteria but it's so transparent so it makes me question if it is. I have a really nice flowhood take all the nessary precautions. Surgical mask,non powder gloves,70% iso freshly launders cloths.i shower n brush my teeth right be for going to work. Run my flowhood for around an hour before working. Have scaple sitting in shot glass of 70 iso. Whip everything down with iso before working spray lysol into the air flame sterilize my scaple.iv never had this problem before. Could it possible be that the mycelium doesn't like the malt extra agar I put it to n I'm having weird growth or mabey I don't let scaple cool down enough before cuting the internal tissue for my fresh fruit and I'm damaging the flesh peice before I inoculate it.
I never have and contamination issues besides the strange transparent ring so I'd greatly appreciate your guys input


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InvisiblebodhisattaMDiscordReddit
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Re: Agar with clear ring around the mycilum [Re: Mycobro420] * 1
    #26981626 - 10/12/20 02:13 PM (3 years, 3 months ago)

Post a picture and try to use some paragraphs. Although I understand it is easier to type the same way a 14 year old girl texts it doesn't make it easy to read for anyone that's going to help you.



It's pretty normal on the leading edge of growth. Especially on the first plate from spores.


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OfflineMycobro420
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Re: Agar with clear ring around the mycilum [Re: bodhisatta]
    #26981666 - 10/12/20 02:33 PM (3 years, 3 months ago)

=https://files.shroomery.org/files/20-42/253470297-IMG_20201012_162809793.jpg][/url]url=http://files.shroomery.org/files/20-42/253470188-IMG_20201012_162831757.jpg][/url]

it's kinda hard to see it my phone camera sucks but it looks like the pictures you posted just not as bad. I also inoculated the plates with the internal tissue from a fresh fruit. Those plates in pics haven't had a transfer yet


Edited by Mycobro420 (10/12/20 02:37 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Agar with clear ring around the mycilum [Re: Mycobro420]
    #26981705 - 10/12/20 02:52 PM (3 years, 3 months ago)

Also i was just giving ya shit but a couple spaces and stuff here and there goes a long way.

The plates look good


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OfflineMycobro420
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Re: Agar with clear ring around the mycilum [Re: bodhisatta]
    #26981718 - 10/12/20 02:59 PM (3 years, 3 months ago)

Ya it's all good. I know I suck at spelling n paragraphs. But my pictures are bad, but there for sure a clear ring around the mycelium. Is that a bacteria, if so can I clean it up. Or is it cause the mycelium doesn't like the nutrition in the medium. Also the plates are colonizing slow. Those are about a week old. I believe it's exactly the same thing in the pics you just shared just not as bad.


Edited by Mycobro420 (10/12/20 03:00 PM)


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Invisiblemind.at.large
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Re: Agar with clear ring around the mycilum [Re: bodhisatta] * 1
    #26981760 - 10/12/20 03:19 PM (3 years, 3 months ago)

Quote:

bodhisatta said:
Although I understand it is easier to type the same way a 14 year old girl texts it doesn't make it easy to read for anyone that's going to help you.



I thought we already told you to stop texting 14 year old girls bod... :drunkdriver:


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InvisibleBaba Yaga
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Re: Agar with clear ring around the mycilum [Re: bodhisatta]
    #26982003 - 10/12/20 05:42 PM (3 years, 3 months ago)

Quote:

bodhisatta said:
The plates look good





Just keep taking transfers and it will sort itself out.


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OfflineMycobro420
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Re: Agar with clear ring around the mycilum [Re: Baba Yaga]
    #27031727 - 11/10/20 02:16 PM (3 years, 2 months ago)

So I have transferred them 4x they still got the clear ring not as bad as before. I also said fuck it n put some to grain the jars fully colonized in 20 days I spawned them so I guess I'll see if they work.iv just never had something like this on my plates. Still confused if it's a contam or something else.


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