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Invisiblejosbos
Registered: 02/16/20
Posts: 134
What is the purpose of a grid while making agar?
    #26981544 - 10/12/20 01:28 PM (3 years, 3 months ago)

Hi folks,

I have seen a lot of videos where people using a grind while pouring there agar in dishes, i wondered what the purpose is of a grid.
So to make it clear the have there petri dishes standing on a grind



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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 10 hours
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Re: What is the purpose of a grid while making agar? [Re: josbos]
    #26981554 - 10/12/20 01:34 PM (3 years, 3 months ago)

it just elevates your work off the floor of your SAB or Flow


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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InvisibleBecky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
Re: What is the purpose of a grid while making agar? [Re: Camera93]
    #26981560 - 10/12/20 01:37 PM (3 years, 3 months ago)

:whathesaid:

I know I am new here, but I grow and that is what I use.


--------------------
- Becky G.

Ditch the double standards boys. Girls like to have fun as much as you do.





Sisters if you find yourself hating, read my bio.


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: What is the purpose of a grid while making agar? [Re: Camera93]
    #26981567 - 10/12/20 01:40 PM (3 years, 3 months ago)

if i clean the floor with 70% do i still need to use a grid?
because i don`t get it... the floor can have contamination but the grid can also have contamination. i don't see the point of it.. or am i missing some thing ?


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: What is the purpose of a grid while making agar? [Re: Becky G. Spot]
    #26981572 - 10/12/20 01:43 PM (3 years, 3 months ago)

-I know I am new here, but I grow and that is what I use. -

Yes i understand that people use a grid i stated that in my question. but the purpose of my question was to find out why its used


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InvisibleBecky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
Re: What is the purpose of a grid while making agar? [Re: josbos]
    #26981575 - 10/12/20 01:45 PM (3 years, 3 months ago)

Quote:

josbos said:
-I know I am new here, but I grow and that is what I use. -

Yes i understand that people use a grid i stated that in my question. but the purpose of my question was to find out why its used




I said "what he said" to the guy above me...

Quote:

Camera93 said:
it just elevates your work off the floor of your SAB or Flow




That is "why" people use them. ....:whathesaid:



Anyways, it's not absolutely necessary I have gone without and been just fine on countless occasions. Good sterile technique is the main concern, with that you can have success in all kinds of setups, and scenarios.

:cheers:


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: What is the purpose of a grid while making agar? [Re: Becky G. Spot]
    #26981584 - 10/12/20 01:50 PM (3 years, 3 months ago)

Thanks for you reply :smile:


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: What is the purpose of a grid while making agar? [Re: Becky G. Spot]
    #26981588 - 10/12/20 01:52 PM (3 years, 3 months ago)

Btw i got also a other question do you know what is the purpose of mycelium selection is ?


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InvisibleLenz
Misunderestimated
I'm a teapot User Gallery

Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: What is the purpose of a grid while making agar? [Re: Becky G. Spot]
    #26981599 - 10/12/20 01:59 PM (3 years, 3 months ago)

70% ISO will sanitize but not sterilize, there are still contaminants present on stuff cleaned with iso. A SAB works by virtue of its design, not by being a completely sterile box.

From what I understand, the reason for working on an elevated grid is that the floor of your SAB still has nasty shit on it that can be kicked up by excess movement and such. This is not so much a concern when working with taller items like grain jars in an SAB but agar plates are short and if you work with them directly on the dirty floor of your SAB you increase the chances of contaminants being kicked up from the floor and into your plate. A grid obviously isn't sterile either but I believe because it's a thin wired grid, there's a much smaller chance of such a thing happening compared to working directly on the floor of your SAB. You can also just use blank petri dishes to elevate your agar for a similar effect.


--------------------
:chems:


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Invisiblejosbos
Registered: 02/16/20
Posts: 134
Re: What is the purpose of a grid while making agar? [Re: Lenz]
    #26981605 - 10/12/20 02:02 PM (3 years, 3 months ago)

Thank you for you input, this makes sense, i can work with this information,  thank you very much!


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