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OfflineAlexjones
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Registered: 07/29/20
Posts: 100
Last seen: 2 years, 1 month
Is my agar too stiff? I have pictures
    #26980342 - 10/11/20 05:49 PM (3 years, 3 months ago)

I added this piece of inner stem to some agar and It has a lot of fuzzy myc  around it but does not look like it is colonizing the agar, as you can see there is two layers so I’m guessing the agar and the brf I mixed with it have separated? The mixture is pretty stiff this my first time doing agar so any advice would be really appreciated
I used agar, brf, and water mixture for these jars



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InvisibleMtbromo
The Bro

Registered: 08/27/20
Posts: 186
Re: Is my agar too stiff? I have pictures [Re: Alexjones]
    #26980460 - 10/11/20 07:22 PM (3 years, 3 months ago)

Looks fine, good growth. Flip the jars upside down after a day or two so the condensation and possible cantams sit in the lid not the agar.

Use malt extract next time instead of BRF


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Knowledge and Healing in a Handful.


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OfflineAlexjones
Stranger


Registered: 07/29/20
Posts: 100
Last seen: 2 years, 1 month
Re: Is my agar too stiff? I have pictures [Re: Mtbromo]
    #26980522 - 10/11/20 07:57 PM (3 years, 3 months ago)

Do most grocery stores carry malt extract I have never heard of it before,I saw someone had used brf so I just substituted it for that


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InvisibleMtbromo
The Bro

Registered: 08/27/20
Posts: 186
Re: Is my agar too stiff? I have pictures [Re: Alexjones]
    #26980561 - 10/11/20 08:26 PM (3 years, 3 months ago)

Yeah in my country sure. Its popular shouldn't be a problem in most western nations


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Knowledge and Healing in a Handful.


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Offlinetopshelf88
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Registered: 10/11/20
Posts: 1
Last seen: 3 years, 3 months
Re: Is my agar too stiff? I have pictures [Re: Alexjones]
    #26980644 - 10/11/20 09:41 PM (3 years, 3 months ago)

it sounds like and looks like some bunk agar you should not have 2 layers the fuzziness is called rhizomorhic growth and that's good but it could also be bacteria. get some new agar and start over but keep watch what happens to that first batch for scientific purposes


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Offlinefightingcherries
I'm a teapot User Gallery


Registered: 10/23/13
Posts: 107
Last seen: 2 years, 7 months
Re: Is my agar too stiff? I have pictures [Re: Alexjones]
    #26980694 - 10/11/20 10:50 PM (3 years, 3 months ago)

According, a vendor I bought agar from, the stiffer the better.  Just like my pops says.


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Is my agar too stiff? I have pictures [Re: topshelf88]
    #26980708 - 10/11/20 11:05 PM (3 years, 3 months ago)

I’ve had agar separate too when using BRF or other stuff that’s harder to completely dissolve like dog food or chunky grain water but it works fine. Malt extract is great cause it dissolves pretty easily and can be found pretty easily online, but BRF also works


How long ago did you inoculate that plate? Wait until the myc starts to crawl across the agar then transfer a tiny piece to a new plate


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OfflineAlexjones
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Registered: 07/29/20
Posts: 100
Last seen: 2 years, 1 month
Re: Is my agar too stiff? I have pictures [Re: mind.at.large]
    #26980856 - 10/12/20 03:42 AM (3 years, 3 months ago)

It’s been about 5 days, the other jars I inoculated with some spore prints those have no sign of growth at all so far. Yeah I hope that fuzzy stuff ain’t bacteria that would suck


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Is my agar too stiff? I have pictures [Re: Alexjones]
    #26981212 - 10/12/20 10:03 AM (3 years, 3 months ago)

5 days is definitely not that long when waiting for spores to germinate. Don’t give up hope yet!

In my experience, bacteria looks more “greasy” than anything else. If there is fuzzy looking stuff coming off a fungal tissue sample, it’s probably fungal mycelium. Not saying there isn’t any bacteria, mold, or anything else hiding in that mycelium, but you will have to wait for it to grow out more to determine that.

Patience, Alex Jones, you paranoid fear mongering weirdo hehe


--------------------
Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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OfflineGan
Wielder of Narya
Male


Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
Re: Is my agar too stiff? I have pictures [Re: Mtbromo]
    #26981332 - 10/12/20 11:20 AM (3 years, 3 months ago)

Quote:

Mtbromo said:
Looks fine, good growth. Flip the jars upside down after a day or two so the condensation and possible cantams sit in the lid not the agar.





Nah you wanna leave them right side up. You want the contams on your agar so you can see them and transfer away from them.


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OfflineAlexjones
Stranger


Registered: 07/29/20
Posts: 100
Last seen: 2 years, 1 month
Re: Is my agar too stiff? I have pictures [Re: mind.at.large]
    #26981650 - 10/12/20 02:24 PM (3 years, 3 months ago)

I’m about to find out what George soros has been putting in the mushrooms now days


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