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InvisibleLenz
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A Log Involving Cubes, WBS, Agar, LI, G2G, Tenacious Molds, Failure & Success?
    #26961180 - 09/29/20 10:23 PM (3 years, 4 months ago)

This log is to track my first serious grow, having only grown once before using the UB tek. My goal with this log is to not only to keep track of things for myself, but also to provide first hand experiental info and photos for other new growers and to receive suggestions from the community.

My objectives throughout this grow are to become familiar and comfortable with most of the beginner/intermediate practical processes used to cultivate cubensis. This includes:
- Pouring plates quickly and cleanly
- Working with agar, from MSS to spore prints to transfers to clones
- Developing an eye for contamination vs clean, organized growth during every phase of the grow
- Developing a sense for the proper moisture levels and preparation of WBS
- Successfully inoculating grains with multiple techniques, from LI to agar wedges to G2G.


At this point I'm a few months into the grow already lol, so here's some background.

Recap:

Start from agar:
- Grow was started a few months ago from a B+ syringe and a GT spore print on pasty plates.
- Only one of 20 B+ plates germinated, took at least 2 weeks but growth looked good.
- GT spore print was taken very sloppily from a fruit from very first grow.
- GT plates showed growth in less than a week, however I overdid it with the spores and failed to streak properly so the growth was a mess.
- Switched to petris, took a bunch of transfers over the course of a few weeks, many looked promising but as they grew out they developed a strange white fuzz and are all now pretty clearly contaminated with a white mold.

Pics (then and now):








- I'm still not exactly sure how I contaminated 95% of my plates with this mold. I was thinking it could be from my spore print but the transfers from my one B+ syringe plate also had this. It's hard to tell if the original untransferred plates were the source of this since they're in pasty plates and it's very hard to see what's going on. That only really leaves my sterile technique at fault but I'm still rather surprised that it was so bad that 95% of my plates ended up with this. In the beginning I wasn't working on a rack, and I worked close to the front of my SAB as well which could be a big source of this contam.


Blenderless LI:
- About a month after I inoculated my very first pasty plates, I had very few plates that I was confident were clean so I turned half a plate into LI. This was the plate:


- The rather unnecessary creation I used:




- Agar was made with a 2% recipe so it was pretty stiff but the nails did a decent job breaking it up. Definitely just going to make my agar softer next time and use a metal lid instead of plastic which leaked a tiny bit.
- I inoculated 5 jars with the LI and 2 jars with a quarter wedge of the plate.


WBS Jars:
- I've been using Fooman's tek and it seems to be working very well so far. I like how quick and simple it is compared to other teks and I seem to get a pretty good hydration level with only around 10% burst grains.

- This is what the LI jars looked like after about 10 days:




- And what the wedge jars looked like after two weeks:




Now:
- Just inoculated 10 plates with a Fiji MSS in order to have something going in case the G2G ends horribly.

- SAB prep:
  - Shut off AC
  - Chase dogs away
  - Wipe all work with ISO paper towel and place on table and then drape in ISO paper towel.
  - Place SAB down over materials.
  - Crack SAB open to spray soapy water from bottom.
  - Let sit for 15 minutes.
  - Put on mask, clean shirt, wash hands, put on gloves, ISO hands and arms then work.

- Using petris poured on 9/13. All look clean, no contam's from pour.
- Was a bit awkward handling both the inoculation loop and the syringe but not severely.
I flamed the loop first, brought it back into the box, cracked open the petri to cool the loop in the agar then quickly flamed the syringe while holding the loop in the other hand, brought the syringe back in, squirted a few drops to cool it, deposited a drop onto the loop, then moved the loop to the other hand and opened the plate once more to zigzag streak.



- Getting ready to G2G.
- Shook the 5 LI jars to check for recovery times and shook the 2 wedge jars since they were around 30%.
- LI jars weren't shaken at all before this and there ended up being a few small chunks of spawn that wouldn't break up. Not sure if that's bacterial or just because they were left to consolidate a bit too long before any kind of shaking.
- Regardless, after 48 hours they look to be recovering fairly well (last two jars are wedge):



- The fifth LI jar recovered faster than any of the others, so I'll be keeping an eye on it and tracking the receiving jars that are inoculated with it.
- I have about 50 jars of WBS that I PC'd over a few days ready to go, I'm hoping 4-5 days isn't too long of a time for them to be sitting around before they get inoculated.
- I'm going to shoot to get around 12 quart jars inoculated per master jar.


For anyone who actually read to here, thanks. Til next time... :pipesmoke2:


--------------------
:chems:


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InvisiblemushboyMDiscord
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Re: A Log Involving Cubes, WBS, Agar, LI, G2G, Tenacious Molds, Failure & Success? [Re: Lenz]
    #26961190 - 09/29/20 10:29 PM (3 years, 4 months ago)

:popcorn:


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Re: A Log Involving Cubes, WBS, Agar, LI, G2G, Tenacious Molds, Failure & Success? [Re: mushboy]
    #26964083 - 10/01/20 03:05 PM (3 years, 4 months ago)

Not a major update but thought I'd pop back in to gripe about my first experience with G2G.

I had 50 jars to inoculate and it took about a good couple hours to do them all, from wiping them all down to waiting between each batch of 10 for the air in my SAB to settle after spraying. After wiping down the 10 jars and the master jar and putting them in the SAB, I covered them in ISO soaked paper towels, sprayed the walls of the box, and let it sit for 10-15 minutes before getting to work.

Now that isn't that bad but the process of G2Ging was just messy.

I have a pretty big 160 QT SAB but even working with ten jars in that space was difficult. I couldn't stack them because I'm using plastic lids and I'm not replacing the lid on my master jar between each pour--I wanted them all on one level so I could quickly pour into each receiving jar and minimize the amount of time my master jar is open. My movement was badly limited by the arm holes of the SAB however and so even after I got all my clean jars in there and let the air settle, I had to move a bunch of jars around just to be able to loosen the lids on all of them. Once I finally got all the lids loosened and the jars in position, I felt like I had kicked up a bunch of contaminants by doing all of that.

It was pretty simple once I started pouring, but my pours were very uneven and I spilled a bit of spawn in the process.

In the future I think I'll put my jars on the table that the SAB will be on and wipe them down and loosen the lids a bit before I put the SAB on top of them. Trying to loosen all those lids in the SAB was basically impossible without moving and touching a bunch of jars, which just doesn't seem too great for a somewhat riskier procedure like G2G. I'm not sure how safe it is to loosen the lids before putting the SAB down but I guess we'll find out.

Anyways, here's what some of the jars look like now:



The myc looks like it's recovering nicely, I'll probably have all these jars get to 100% at different times cause of the wildly differing amounts of colonized grain I was able to get in them lol. I'm assuming that any contaminants that might've entered during the G2G won't be obvious this soon. Fingers crossed.

I had a tiny bit of leftover spawn in each of the master jars after the G2G so I decided to hydrate some coco and verm real quick and spawn it in a plastic tupperware quart container for shits and gigs and maybe even something clone worthy. Ended up being about 75g of hydrated coco/verm and 65g of colonized WBS. Probably 1:3 by volume though. Sub is around 2" deep. I misted it a bit and I'm probably gonna keep the lid closed until the myc colonizes the sub a bit more.



No change on the Fiji MSS plates, really need to get a IR thermometer and stop pouring lumpy plates though.

:balloon:


--------------------
:chems:


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InvisibleMunchauzen
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Re: A Log Involving Cubes, WBS, Agar, LI, G2G, Tenacious Molds, Failure & Success? [Re: Lenz]
    #26971797 - 10/06/20 09:41 AM (3 years, 4 months ago)

Quote:

Lenz said:
- The rather unnecessary creation I used:





thats awesome :rofl:

I bet it works well!


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: A Log Involving Cubes, WBS, Agar, LI, G2G, Tenacious Molds, Failure & Success? [Re: Munchauzen]
    #26980142 - 10/11/20 02:55 PM (3 years, 4 months ago)

Quote:

Munchauzen said:

thats awesome :rofl:

I bet it works well!





Thanks Munch! Blenderless LI is a real game changer, I'm glad I tried it immediately, I would probably still be waiting for my receiving jars to colonize right now if I went A2G before G2G.

And the stabby lid worked alright haha but between slight leaks from the plastic lid and the rust forming on the nails I probably won't use it again for the time being. Maybe if I find a random handful of SS nails in the woods or something I'll try it again lol


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:chems:


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: A Log Involving Cubes, WBS, Agar, LI, G2G, Tenacious Molds, Failure & Success? [Re: Lenz]
    #26980204 - 10/11/20 03:36 PM (3 years, 4 months ago)

Long overdue update:


- Almost all of the G2G jars hit 100% in about 10 days, about half of them needed a good shake due to uneven colonization but they recovered well within 48 hours and caught up to the rest of the jars right on time.

- They looked pretty good to my eyes, I don't doubt that they're probably slightly bacterial but the quick recovery and colonization makes me not too worried. I'm really surprised/happy that they all colonized well, I was nervous about G2Ging this many jars for my first time but it seems like it paid off. No obvious wet spots, myc isn't too thick or creamy looking, nothing to indicate mold, etc.

Here's what they looked like 2 days before spawn:


- I spawned them all to 6 monotubs, each tub ended up getting 7 qt jars that were filled to about 80% of a quart.

- Spawning them took way longer than I expected, from drilling all the holes in the monos to making the liners to prepping the coir to breaking up the jars to mixing the sub without making a mess, but I was able to get all the tubs done late in the night.

- Speaking of drilling, I went with Pasty's EZ dialed in monotub design. The one mono I'd done before I started this grow I had went with Bod's unmodded mono's but I felt like I had to be misting constantly and checking on it. Might be due to the 1.5" sub depth and me fucking with the tub all the time but I liked Pasty's design.

- The room they're in is usually around 75 degrees and 40-50% RH, feel like they'll do pretty well in those conditions.

- Also, regarding liners, it was a bit of a bitch to cut and trim them, might try garbage bags next time, I like the idea of being able to mix everything right in the bag.

Here's a pic of one of the tubs. Sub depth is around 4", took about 15qt of sub to get there so my spawn:sub ratio is around 1:3.



- The tupperware actually started pinning yesterday!!!


- I used hella old coir that was sitting around halfway hydrated for this (hydrated it fully before spawning), so it's good to see I'm actually getting some pins.

- I was pretty worried I wouldn't get pins cause I was having trouble getting the surface conditions and FAE right. The myc was getting super aerial and weird before the pins came up, like standing up in little curls and I think I let it get too dry and then overmisted to try and compensate and the myc got a bit bruised and discolored. Glad to be getting something though.

- Here's what it looked like a couple days before pins:


- Not a great pic but in the top right you can see a little yellow drop on top of the myc, I assumed that was metabolites. Strange appearance of the myc and the tiny yellow drops are what I guess is a lack of proper FAE and maybe some bacterial contam. I had left the lid cracked on this for FAE but I think the height of the container may have inhibited it so I put some holes above the substrate and near the top and that seems to be working bit better.


And the 2 shoeboxes are chugging along as well:


- These were inoculated with the A2G jars shown previously, 1:2 spawn to sub ratio. 1 quart jar in each.

- It's been 6 days since I spawned them and the growth looks decent now, the patchiness in the back may be cause for concern but I'm gonna wait and see what they're looking like in a few more days.


- Otherwise I'm hyped that everything is getting into final stages, really hoping nothing goes wrong with the monos. The Fiji spore syringe plates still show no sign of growth, this is the second syringe I've been unable to germinate on agar for some reason. I'm really not sure what it is, I'm letting the syringe cool before putting a drop on my inoculation loop so the spores aren't being killed by the heat, and they're from a reputable vendor. Might try a softer agar recipe next time.

As always, thoughts on any of the growth/pics or suggestions for streamlining the bulk growing process is welcome, thanks for tuning in.


--------------------
:chems:


Edited by Lenz (10/11/20 03:40 PM)


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