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JennyJackston
Stranger

Registered: 09/28/20
Posts: 43
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Almost ALL of my Agar dishes have blue contam. What can I do next?
#26980153 - 10/11/20 03:04 PM (3 years, 3 months ago) |
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Last weekend I inoculated about 30 or so agar dishes. A week later, the majority of them have blue contam in them.
Some came from spore prints that I made about 3 years ago. Maybe these were not in the most sterile of enviroments as I was just starting out. The prints were on glass and kept in plastic zip lock bags.
The rest were inoculated with spore syringes.
The agar is in two types of dishes: Glass petri dishes and large plastic Deli type dishes.
I think almost all of the ones in the plastic dishes are shwoing blue contam in large places.
The glass petri dishes seem to be ok.
Before inoculation, I washed the dishes in hot soapy water and then cleaned them with 70% Iso.
I can’t PC the plastic dishes, they will melt.
I have a SAB that I sprayed with soapy water a few minutes before I did the work. I took a shower, wore a lab coat, a hair net, a surgical mask, disposable gloves, cleaned my hands and gloves with Iso. Heat sterilised the needles on the syringes and the metal tool to transfer spores from the spore print.
My two questions are: Can in rescue the white mycelium from the contaminated dishes and transfer to clean agar dishes?
What am I doing wrong here? I thought I was doing everything by the book and yet I am STILL getting the majority of failures.
Its like not matter what I do, I get no success.
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
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Re: Almost ALL of my Agar dishes have blue contam. What can I do next? [Re: JennyJackston]
#26980164 - 10/11/20 03:09 PM (3 years, 3 months ago) |
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Quote:
JennyJackston said: My two questions are: Can in rescue the white mycelium from the contaminated dishes and transfer to clean agar dishes?
That's the entire purpose of using agar.
Quote:
What am I doing wrong here?
We can't see you're doing without pictures.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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mad_dingo
professional mango


Registered: 08/19/20
Posts: 67
Last seen: 2 months, 11 days
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Re: Almost ALL of my Agar dishes have blue contam. What can I do next? [Re: JennyJackston]
#26980212 - 10/11/20 03:40 PM (3 years, 3 months ago) |
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Quote:
JennyJackston said:
I can’t PC the plastic dishes, they will melt.
What am I doing wrong here? I thought I was doing everything by the book and yet I am STILL getting the majority of failures.
Well. You don't do everything by the book, because then you would know that you _can_ sterilize plastic dishes if they are made of Polypropylen PP (indicated by the 5 in the recycle triangle). Just sanitizing them is setting yourself up for failure.
You need to slow down and go back to reading about the basics https://www.shroomery.org/forums/showflat.php/Number/24144021
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