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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Solipsis]
    #26967001 - 10/03/20 06:17 AM (3 years, 4 months ago)

Well idk if it likes the hwfp but it definitely didn’t hurt. Overlay seems to be starting though.


Going with extremely low spawn for the next few.


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OfflineOne of Us
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Re: Psilocybe natalensis [Re: A.k.a]
    #26967128 - 10/03/20 08:07 AM (3 years, 4 months ago)

Something I think worth mentioning here is that, due to a refrigerator malfunction a few weeks ago, about ten cultures that I've been working with became encased in ice for at least a week before I noticed. There were two stacks of five or six petris, each in its own ziploc.

After freeing them from their icy prison, none of them looked really good. In fact I thought all of them were gonners. The media within them had frozen solid. When the agar froze, it pulled all of the moisture out of it, so when it thawed, the insides of the petris were flooded, and the remaining agar was very rubbery. It did not look good at all.

Thankfully, I can be a lazy slob sometimes, and I didn't immediately throw the soupy mess petris out. Within 3-4 days, all had visible growth restarting from the center of the dish. All of them, that is, except for my two natalensis cultures. And both of these cultures were located in the middle of the frozen petri stacks, where I would assume they would be more insulated than the others.

I guess my point is that the natalensis cultures at least appear to be less tolerant of (very) cold temperatures compared to the other cube cultures, all of which I was able to recover.

Anyway, that's why you haven't seen any recent Nats from me. I'm gonna start some from spores today, and dig around in my petri hoard to see if I can find any overgrown copies of my nat clones.


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Invisiblepsilocyclops
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Re: Psilocybe natalensis [Re: One of Us]
    #26970031 - 10/05/20 01:10 AM (3 years, 4 months ago)

Update on my tub: my overlay growths are getting downright coral-like and are some of the most beautiful formations I have ever seen in nature. Feeling blessed to be a member of this community and feeling blessed to be a human on earth in this time of new and exciting fungal discovery. Without further adieu, here are the pictures! As a forenote, these growths have grown large and dense enough to dry out, has anybody bioassayed these growths? They seem so potent. I cut all of mine off as others have and have begun to initiate fruiting conditions. I also have a small ziploc container of natalensis with a nice casing of vermiculite to hopefully avoid this overlay issue.

BTW that picture of my shoebox is post-cleanup. These growths are starting to dry out quite a bit and it’s looking like i’m gonna at least get a couple grams out of it and if they’re as potent as they look they should be awesome! ALSO WORTH NOTING: these growths came from a “ropey” isolate i took from multispore agar. Here’s the isolate I took as well as a picture of it grown out on agar for those that haven’t been keeping track.


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: psilocyclops]
    #26970074 - 10/05/20 02:24 AM (3 years, 4 months ago)

That’s actually really good as far as overlay, a lot of times they form solid pumps that cover the surface.

I’ve also been pulling myc off mine and thinking about eating it cuz it’s bluing strong, I don’t think anybody has tried it yet so you’d be the first.


It’s pulling off deep chunks of coir this time though.


Next project I guess will be taking rhizo and tomentose transfers to grow out from this first ever normal plate.


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Edited by A.k.a (10/05/20 11:17 AM)


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OfflineSolipsis
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Re: Psilocybe natalensis [Re: A.k.a]
    #26970598 - 10/05/20 01:16 PM (3 years, 4 months ago)

So i checked and confirmed... in that original description of natalensis in Mycotaxon in 1999, caulocystidia ARE described. Resembling the cheilocystidia, bifurcated at the base, 18-30 µm long, etc..

But as far as I can tell, the description of cubensis according to Guzmán says nothing about caulocystidia.

I don't know how literal it should be taken that microscopic difference like regarding cystidia are what telling Psilocybe species apart is based in i.e. how specific are these caulocystidia and do cubensis never have them?

Would it then be expected that there are at least some differences in DNA, part of which could then perhaps be associated with the caulocystidia?

Wondering if it is quite impossible then if there turns out to be as good as no difference in barcode sequences, or what is the overriding factor?

I invariably get humbled by the mycology and possibly or likely I am just missing something here but i would love the explanation. :smile:

(If not, idk if there was a component of this in Advanced Mycology it should be posted in...)



Sorry never mind - seems the caulocystidia description was not included in the monograph nor supplements ive seen.. but I do see they are described in Noordeloos book.

So, my bad.

It does kinda turn my question upside down, if still so much of the microscopy is a match, then how much of the barcode sequences could be afforded to differ and still call it flukes - since they are so similar in the ways that should define them/it.

Will need to try crossing better again then, particularly with a monokaryon thats produced cube-cube crosses.. bit of a bummer if turns out to be a cube yet cannot be crossed.

---

Anyway I forgot to check for caulocystidia when I grew my first strain of Nats, however i got another strain which should not be quite as cube-like as the one i grew...
I know macroscopic features can be misleading but its still intriguing. I wonder to what extent this fungus/these fungi have a casual look and business look haha - epigenetically toggled and more related to how different wild look can be for so many mushrooms, as good as all of em it seems. :laugh:
And under some conditions it really quickly loses annulus/ring, so easily that it might as well not have been on there at all? hmm

Also, what if they are reproductively compatible despite being different species and we've been having a ton of hybrids.... :/


Edited by Solipsis (10/05/20 02:30 PM)


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Invisiblec10h12n2o
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Re: Psilocybe natalensis [Re: Solipsis]
    #26970942 - 10/05/20 05:01 PM (3 years, 4 months ago)



Crackatoa genetics


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: c10h12n2o]
    #26970964 - 10/05/20 05:24 PM (3 years, 4 months ago)

Daaaamn look at those spore drops!

So what’s the deal with that tub?? It looks like the sub is super deep.


I’m so jealous of all you guys getting awesome tubs.


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Invisiblec10h12n2o
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Re: Psilocybe natalensis [Re: A.k.a]
    #26971128 - 10/05/20 07:05 PM (3 years, 4 months ago)

Its deep af. Neglect tek. Trayed it up and left town for a few weeks haha


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflineGrimsweeper
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Re: Psilocybe natalensis [Re: c10h12n2o]
    #26971272 - 10/05/20 08:32 PM (3 years, 4 months ago)

Badass c10 :rockon:


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Re: Psilocybe natalensis [Re: Grimsweeper]
    #26971356 - 10/05/20 09:37 PM (3 years, 4 months ago)



Nat plate pin. Just transferred the clone to agar and swabbed 2 plates with the spores.


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Re: Psilocybe natalensis [Re: Wall.E]
    #26971433 - 10/05/20 11:36 PM (3 years, 4 months ago)

Pasty plate pins are so much prettier than standard plate pins


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

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Re: Psilocybe natalensis [Re: c10h12n2o]
    #26971491 - 10/06/20 01:40 AM (3 years, 4 months ago)

Lol yeah not all flat and sideways.


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Re: Psilocybe natalensis [Re: A.k.a]
    #26973145 - 10/07/20 02:56 AM (3 years, 4 months ago)

So this is pretty much par for the course for me. The only differences are the overlay isn’t totally filling in the surface and there’s no myc climbing the walls which is normally what it’s all about.



Pulling off the overlay took out a ton of coir this time but it looks like the first few spots I pulled are filling in pretty nicely.

So hwfp isn’t my magic bullet.


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: A.k.a]
    #26977936 - 10/10/20 04:05 AM (3 years, 4 months ago)

Finally got a culture that looks workable, took rhizo and tomentose sections the other day and dropped the big rhizo chunk to grain right after this picture.


It’s kind of shocking that this is the same culture two plates ago.


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Edited by A.k.a (10/10/20 04:07 AM)


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OfflineGrimsweeper
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Re: Psilocybe natalensis [Re: A.k.a]
    #26978084 - 10/10/20 07:42 AM (3 years, 4 months ago)

I could be totally wrong but I'm guessing tomentose will give you better surface conditions for fruiting. Are you going to run them both?


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Re: Psilocybe natalensis [Re: Grimsweeper]
    #26978301 - 10/10/20 10:33 AM (3 years, 4 months ago)

I think you’re right cuz last time I did this with ms cultures tomentose overlayed much less.


Once the wedges I took grow out I am gonna run them both but I figured I might as well grain this plate too.


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Offlinekarri0n
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Re: Psilocybe natalensis [Re: A.k.a]
    #26979326 - 10/11/20 12:13 AM (3 years, 4 months ago)

Is overlay a known common issue with NSS Cubes, or is this *only* happening with what we are thinking is the distinct Natalensis species?

Early in the thread I also recall folks stating it seemed like the myc of the Natalensis was super aggressive and just eating through contams(and liners lol) like Oysters. Does that still seem to be the case?

If this species which is new to mush cult has such strong and aggressive mycelium, then is it fair to think that a couple years down the line with some good isolation practices we could have the "next cube"?

It would certainly be VERY interesting to have another species that produces large yields and can be cultivated nearly as easily as a cube.


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OfflinePsiloPsychIn
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Re: Psilocybe natalensis [Re: c10h12n2o]
    #26979393 - 10/11/20 02:10 AM (3 years, 4 months ago)

Quote:

c10h12n2o said:


Crackatoa genetics




Well Done!
:seriousthumbsup:


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Re: Psilocybe natalensis [Re: PsiloPsychIn]
    #26979449 - 10/11/20 04:11 AM (3 years, 4 months ago)

Would anyone be willing to weigh in and tell me what I have here? Where should I pull from?

Nat plate 1





Nat plate 2





I'm gonna grab the rhizo growth at 12 and 2 on the respective plates, but I wanna know if there's any benefit to grabbing the panaleous looking mycelium at 7-8 o'clock on the second plate.

Any help would be appreciated thanks


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Re: Psilocybe natalensis [Re: Wall.E]
    #26979693 - 10/11/20 09:18 AM (3 years, 4 months ago)

Those all look good man, super tame myc. I kinda like 6-7 on both but I’ve had horrible instincts for natalensis myc so far.


Karrion I think the crazy overlay is mostly with actual natalensis. I’ve gotten it on every grow I’ve done so far, and the myc has eaten through the liner every time. It also climbs the lid of plates and the walls of tubs like crazy.

Mine haven’t eaten contam but they definitely don’t seem as affected by it as most mushrooms. When green shows up it tends to stay contained to one small spot and not harm anything. Idk for sure but I kinda think that might be cuz of how thick and dense the myc tends to get and makes it tough for the mold to spread.


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