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Offlineoname_wa
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Registered: 03/15/20
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Jars stopped colonizing
    #26974776 - 10/08/20 01:06 AM (3 years, 4 months ago)

I am fairly new to cultivating, but have had a few great flushes and want to continuing learning!

I started some PE jars over a month ago expecting them to be fully colonized and ready to mix with sub. I used 5 ccs to inoculate each jar in a SAB after hydrating and pressure cooking my grain at 15 psi for 90 minutes. After 6-7 days they started pinning and colonizing beautifully but abruptly stopped. Is it bacteria or contaminants? I don't see any noticeable signs but could definitely be missing something. They have been in dark at 70-74 degrees in my closet with minimal natural sunlight. Do the jars need complete darkness, I have been reading lately that natural sunlight is not bad at any stage of growth. Is this true?

If no signs of contaminants, should I proceed even with some grains not colonized? Should I wait longer?

A lot of questions, I know! Like I said Im a newbie!

Any input would be kindly appreciated!



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OfflinePearl
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Registered: 05/28/20
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Re: Jars stopped colonizing [Re: oname_wa]
    #26974790 - 10/08/20 01:38 AM (3 years, 4 months ago)

Light isn't bad, no worries about them being exposed to it.

Could be my eyes and the smallish pics but it looks probably OK for now and I'd shake and wait.

When were they inoculated? What's the lid setup/can they breathe?  Have you shaken them at all?


*edited for clarity


Edited by Pearl (10/08/20 06:03 AM)


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OfflineForresterM
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Re: Jars stopped colonizing [Re: Pearl]
    #26974838 - 10/08/20 02:34 AM (3 years, 4 months ago)

No, do not proceed with uncolonized grains.  It's probably bacterial, but those pics don't enlarge enough for me to see very well.  Could also be not enough air as mentioned.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlineoname_wa
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Re: Jars stopped colonizing [Re: Pearl]
    #26976284 - 10/08/20 11:23 PM (3 years, 4 months ago)

Thanks!
I shook the jar at 30%. I colonized on 8/12/20. So its been a minute!

Here is a closer up of jars.



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OfflineMrTheseThings
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Registered: 06/21/20
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Re: Jars stopped colonizing [Re: oname_wa]
    #26976294 - 10/08/20 11:35 PM (3 years, 4 months ago)

I vote bacterial . Maybe air exchange but it looks bacterial to my eyes.


--------------------
These things infected me... and now I have to  grow them.


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Offlinechowster
Happy Farmer

Registered: 11/20/13
Posts: 182
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Re: Jars stopped colonizing [Re: MrTheseThings]
    #26976319 - 10/09/20 12:04 AM (3 years, 4 months ago)

Can't see too well, but see in your most recent pictures in the third photo, the really wispy white, almost grey growths? Looks like mold to me.


--------------------
:mushroom2: :cookiemonster: :mushroom2:


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OfflineForresterM
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Re: Jars stopped colonizing [Re: chowster]
    #26976339 - 10/09/20 12:39 AM (3 years, 4 months ago)

Yeah that's quite a long time, if it didn't recover from a shake it's almost definitely contamination.  I still can't see well enough to tell what, but you can be fairly sure they're contaminated.  I would start over.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlineoname_wa
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Re: Jars stopped colonizing [Re: chowster]
    #26977724 - 10/09/20 10:25 PM (3 years, 4 months ago)

I was kind of afraid that's what I am seeing. Would pressure cooking the grains longer than 90 min help. This was my first attempt with a SAB.

Definitely trying again. Hopefully next attempt will be more successful. Any suggestions when inoculation or prep of the grain jars?


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OfflineDonkeyHodie
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Registered: 03/30/18
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Re: Jars stopped colonizing [Re: oname_wa]
    #26978117 - 10/10/20 08:12 AM (3 years, 4 months ago)

Did you use spores? Spores are notoriously unreliable on grains.


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InvisibleSir Pentinite
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Registered: 05/15/19
Posts: 525
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Re: Jars stopped colonizing [Re: oname_wa]
    #26978269 - 10/10/20 10:15 AM (3 years, 4 months ago)

Quote:

oname_wa said:
I used 5 ccs to inoculate each jar




Of spore solution? There's your problem.

Spores aren't clean. It's a big gamble putting them directly on grain. I would say it fails as often as it works and a lot of what "works" is still at least bacterial. Often you can still get something out of bacterial spawn, but mold is a deal-breaker and I think that's what you have.


--------------------
"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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Offlineoname_wa
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Re: Jars stopped colonizing [Re: Sir Pentinite]
    #26979940 - 10/11/20 12:25 PM (3 years, 4 months ago)

Yes. Using Spore syringe. Trying to get some fruits to make the switch to agar.

Would something like brown rice be more successful with liquid spores?


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OfflineForresterM
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Re: Jars stopped colonizing [Re: oname_wa]
    #26979949 - 10/11/20 12:35 PM (3 years, 4 months ago)

It doesn't matter what grain you use, spore solution isn't sterile, so you're almost definitely going to be fighting contamination to some degree.  While it can work sometimes, it's only by luck.  The more spore solution you use, the more advantage you're giving the bacteria that's in there.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinebloodcountess
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Registered: 10/04/20
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Re: Jars stopped colonizing [Re: oname_wa]
    #26980093 - 10/11/20 02:16 PM (3 years, 4 months ago)

you dont need fruits to start agar you just need an inoculation loop and a spore syringe. follow c10's agar tek he has a gif that shows how to scrape plates with an inoculation loop using a drop from spore syringe. i am currently having a hard time with APE colonizing my millet grains (my golden teacher colonized really well) and am going to be following c10's agar tek. tek here: https://www.shroomery.org/forums/showflat.php/Number/24330957/fpart/all/vc/1

also fyi 5cc per jar is a lot. the general cosensus is 1cc per jar

good luck


Edited by bloodcountess (10/11/20 02:19 PM)


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OfflineCheesekiller
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Re: Jars stopped colonizing [Re: bloodcountess]
    #26980102 - 10/11/20 02:21 PM (3 years, 4 months ago)

I agree with MrTheseThings, Bacteria usually stops spawn.  Either that or some other kind of contamination.

-Ck


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup


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InvisibleMtbromo
The Bro

Registered: 08/27/20
Posts: 186
Re: Jars stopped colonizing [Re: Cheesekiller]
    #26980189 - 10/11/20 03:27 PM (3 years, 4 months ago)

MSS are always a risk. Put it to agar 1st. If it stops colonizing its usually always contams not a light issue. Some contams arent visable.

You can take the colonized grains and separate them and make a mini fuck around tub in a tiny shoebox or take away container with some coir, see if anything will grow just as a non serious grow


--------------------
Knowledge and Healing in a Handful.


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OfflineLieutenant Pan
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Registered: 09/11/20
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Re: Jars stopped colonizing [Re: Mtbromo]
    #26980221 - 10/11/20 03:51 PM (3 years, 4 months ago)

Those grains are too wet make them right and you'll be good.


--------------------
I never thanked ya fer saving my life.


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Offlineoname_wa
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Re: Jars stopped colonizing [Re: Lieutenant Pan]
    #26981328 - 10/12/20 11:18 AM (3 years, 4 months ago)

thanks for the guidance!!

I'll look into the agar tek and start there next.


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Offlineoname_wa
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Re: Jars stopped colonizing [Re: Lieutenant Pan]
    #26981338 - 10/12/20 11:24 AM (3 years, 4 months ago)

Quote:

Lieutenant Pan said:
Those grains are too wet make them right and you'll be good.





How long should I let grains soak in water before pressure cooking?


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OfflineForresterM
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Re: Jars stopped colonizing [Re: oname_wa]
    #26981342 - 10/12/20 11:26 AM (3 years, 4 months ago)

It's not the soak time, it's not letting them steam dry enough before bottling them up for the PC cycle that leaves them too wet. 

After you simmer them and strain off the hot water, lay the grains out on a towel or screen or something and let all the steam come off them until they are fairly dry.  Then load up jars.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineLieutenant Pan
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Registered: 09/11/20
Posts: 339
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Re: Jars stopped colonizing [Re: Forrester]
    #26984255 - 10/13/20 10:44 PM (3 years, 4 months ago)

Just hard boil for 20 minutes once you see the water level went down an inch you soaked enough.  Watch RR's lets grow muchrooms grain prep video and you will be a grain master.


--------------------
I never thanked ya fer saving my life.


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