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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 11 days
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Quote:
verum subsequentis said: I personally think LI is harder to pull off than LC. I wouldn't say there is any bar that must be reached. I just advise noobs to get really reliable with agar to grain before fucking with liquids.
Agree with this, it's too often you see newer growers thinking they can just throw a mushroom tissue piece or some spore solution in LC broth and be good to go.
And even if they don't do that, many still think you can see contamination in LC and that if it's clear and not cloudy it's clean.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26976402 - 10/09/20 03:04 AM (3 years, 4 months ago) |
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Quote:
Forrester said: many still think you can see contamination in LC and that if it's clear and not cloudy it's clean.
Many = Every Microbiologist on earth including all of us that actually investigate and research the stuff that we have definitive opinions about.
The question now becomes, why did you apparently think that turbidity wasn't directly related microbial mass in a liquid media? If you have some document or research that support that notion then I'd be happy to look I to it 
https://sciencing.com/turbidity-indicate-microbiology-12703.html
Quoted this from the article in the link:
"While turbidity describes the general measure of suspended particles in a liquid, it is not reserved only for water, or for visible particles. Microbiologists use turbidity as a measure of cell density within a culture sample. Microbiologists use machines called photometers and spectrophotometers that shine different types of light through culture samples to determine turbidity. The general assumption is that the higher the turbidity, the higher number of cells within the culture."
-------------------- Cakes inside Water Tub
Edited by Mateja (10/09/20 06:37 AM)
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 11 days
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26977274 - 10/09/20 04:25 PM (3 years, 4 months ago) |
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Oh dear God here we go again...
Nope. Not gonna do it. Go around spouting all the bullshit you want.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26977276 - 10/09/20 04:25 PM (3 years, 4 months ago) |
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Again?
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 11 days
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Arguing with this guy. I have better things to do. If anyone else that doesn't put words in my mouth and ignore everything I say in response wants to disagree, I'm happy to oblige, but not with this dude again, it's not worth my time.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26977353 - 10/09/20 05:18 PM (3 years, 4 months ago) |
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Quote:
Forrester said: Oh dear God here we go again...
Nope. Not gonna do it. Go around spouting all the bullshit you want.

And there you go again as well, personal opinions worded as scientific facts and then when I respond to the fallacy in your claim and back by response up with scientific documents and research and ask you about info or documents that your claim may originate from then you just start addressing everything non related but somehow always miss to adress key points in our conversations about science. That's weird considering growers look for your participation and value your willingness to answer posts and I'd assume there's no reason why you immediately feel the need to shut me completely down as soon as I challange one of your points or claims or whatever science related I'm glad you're not a moderator at least I guess this cancle culture is nonsense is contaminating the knowledge some of us are seeking, or in some cases just clarifications. I didn't come do throw tomatoes I have literally tons upon tons of opportunities to throw tomatoes on unfortunately designed claims, opinions, scientific formulations etc but I was mostly curious about how we can have arrived at seemingly exact opposite understanding of what turbidity and lack thereof means in context of colonizing LC broths. I'm still up for factual discussions/debates but I don't see you as my enemy nor anything other horrible that one can conjure in the moment of derailing into off topic.
I find talks about colonizing LC broths to be a fascinating as of lately, pardon my enthusiasm and fact checking insentives, I'm just being curious mayn
-------------------- Cakes inside Water Tub
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 11 days
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26977384 - 10/09/20 05:40 PM (3 years, 4 months ago) |
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All I said was you couldn't tell an LC was clean by the fact that it wasn't cloudy.
By your logic, my tap water should be sterile because it isn't cloudy.
That's all I need to say, end of discussion. Post all the links you want, it doesn't make the only statement I made, listed above and repeated for you, false.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26977468 - 10/09/20 06:45 PM (3 years, 4 months ago) |
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Quote:
Forrester said: All I said was you couldn't tell an LC was clean by the fact that it wasn't cloudy.
So this is one statement that I feel doesn't hold up from a scientific point of view. If you start with a freshly sterilized LC that has a very clear broth and after sufficient incubation time you still have the same clear broth then by all scientific research it should be concluded that your LC broth has no growth activity of any kind. This means that your sterilized LC still remains as sterile as it was fresh outta PC.
Quote:
By your logic, my tap water should be sterile because it isn't cloudy.
Now you're both misrepresenting my reasoning on the topic and you're misinterpreting the definitions of the science. First off it's not my logic that freshly poured tap water is sterile because it's not cloudy. But if your freshly poured tap water was incubated for a few days and still no turbidity was detectable then the science behind it will conclude that your tap water in that sample actually lacks growth activity entirely. But then again I can't argue using valid science on you're point about the tap water since tap water alone isn't considered a liquid growh medium thus can't be used as a tool to measure growth activity. Seems like you just need to refresh you're memory a bit on the subject of LC broth so you can express your views more clearly and be able to construct your arguments to be scientifically valid and thus can be discussed productively.
Now I feel we're getting somewhere with this, like we understand each other's posts a little better, I feel that we're learning and that's awsome
-------------------- Cakes inside Water Tub
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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26977849 - 10/10/20 01:36 AM (3 years, 4 months ago) |
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Quote:
Forrester said: Oh dear God here we go again...
I just read this thread again from start to finish and this time when I read everything I not only realized it's time for me to take a longer look at my self than I first thought  What worries me is I wasn't even aware of how much I misinterpreted everyone and the egoic nonsense Im doing is some of the cringiest most pathetic crap I've caught myself doing so far (I hope) hence what worries me, how many other times have I clearly crossed to line and it totally didn't register with me. JEEZ. I really hope this is the wake up call I needed to make a better change.
Quote:
verum subsequentis said: Again?
Hate to admit but its a pattern I really wish wasn't there, obviously I wish to be in a state where I can trust my own judgement in being able to recognize this ego maniac in my posting before Its too late, I can't even try to polish this turd I apologize to everyone in this thread and beyond, I don't even have the right to promise anything cause I can only hope that I will never again treat fellow cultivators this bad...
-------------------- Cakes inside Water Tub
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: LC test to agar. Clean or bacteria? [Re: Mateja] 1
#26978427 - 10/10/20 12:01 PM (3 years, 4 months ago) |
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Nah man, don't be lame. You do you it's alright. The only thing you need to change is the current pic you just put for your avatar and put back the war-fucking-mongering Jesus Christ cuz that's what's all about.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26978598 - 10/10/20 02:09 PM (3 years, 4 months ago) |
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If you want peace prepare for war
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 3 hours, 2 minutes
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Re: LC test to agar. Clean or bacteria? [Re: JHOVA]
#26978651 - 10/10/20 02:52 PM (3 years, 4 months ago) |
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The wars always break out when LC comes up
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: LC test to agar. Clean or bacteria? [Re: Asura]
#26979080 - 10/10/20 08:04 PM (3 years, 4 months ago) |
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I work with LC all the time and it almost never starts a fight.
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trippleblack
Stranger

Registered: 12/01/19
Posts: 367
Last seen: 17 hours, 31 minutes
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i have run tons of lc lately
always always always test on a plate before dropping it onto anything bulk.. there is no way you can always tell if a lc is clean by just looking at it; even though it usually is if agar is good.
best way to test is apply a 1/4 inch dash in the center of the petri using an inoculation loop. maksure you to stir lc jar before testing. you will have your answer in 3 to 4 days. bacteria or mold slow the culture way down, you can tell very quickly if your culture is clean or not, that dash will be a perfect line of mycelium and the dash should spread out over the whole plate eventually.
Edited by trippleblack (10/10/20 08:21 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979150 - 10/10/20 09:36 PM (3 years, 4 months ago) |
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i prefer a zig zag but it does the same thing.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 3 hours, 2 minutes
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Quote:
verum subsequentis said: I work with LC all the time and it almost never starts a fight.
LC is for lovers
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: LC test to agar. Clean or bacteria? [Re: Asura]
#26979161 - 10/10/20 09:48 PM (3 years, 4 months ago) |
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should be. If not it'll make a lover out of you if you stick with it.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979490 - 10/11/20 05:35 AM (3 years, 4 months ago) |
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Quote:
trippleblack said: i have run tons of lc lately
always always always test on a plate before dropping it onto anything bulk.. there is no way you can always tell if a lc is clean by just looking at it; even though it usually is if agar is good.
best way to test is apply a 1/4 inch dash in the center of the petri using an inoculation loop. maksure you to stir lc jar before testing. you will have your answer in 3 to 4 days. bacteria or mold slow the culture way down, you can tell very quickly if your culture is clean or not, that dash will be a perfect line of mycelium and the dash should spread out over the whole plate eventually.
You guys saying it's impossible to know if the LC is good should try my LC tek for some months, start making 0.2% strength LME broths and maybe quit using stirrers for a while just for experimentation's sake. Then come here and tell me if you still can't spot the funk in an LC.
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trippleblack
Stranger

Registered: 12/01/19
Posts: 367
Last seen: 17 hours, 31 minutes
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26979571 - 10/11/20 07:45 AM (3 years, 4 months ago) |
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Quote:
Josex said:
Quote:
trippleblack said: i have run tons of lc lately
always always always test on a plate before dropping it onto anything bulk.. there is no way you can always tell if a lc is clean by just looking at it; even though it usually is if agar is good.
best way to test is apply a 1/4 inch dash in the center of the petri using an inoculation loop. maksure you to stir lc jar before testing. you will have your answer in 3 to 4 days. bacteria or mold slow the culture way down, you can tell very quickly if your culture is clean or not, that dash will be a perfect line of mycelium and the dash should spread out over the whole plate eventually.
You guys saying it's impossible to know if the LC is good should try my LC tek for some months, start making 0.2% strength LME broths and maybe quit using stirrers for a while just for experimentation's sake. Then come here and tell me if you still can't spot the funk in an LC.
why would anyone not test a liquid culture going to bulk? it only takes 3 or 4 days. not testing my bulk lc would be lazy for me not to do.
why would anyone not use magnetic stirrers? around a 2% solution of culture media is standard.. i run quart jars they are done within a week. I tested clear jars hiding mold before, rare but happens.
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