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sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
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Re: Some notes about LCs [Re: micelio] 1
#27095968 - 12/18/20 02:50 PM (3 years, 1 month ago) |
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Thanks! That info should be front-and-center for anyone using LC!
Edit: I found a paper from 1995 that documents successfully storing mycelial plugs of many species for 7 years in 2ml vials of sterile water at 5C. They also note "Refrigeration may not be necessary. Boesewinkel (1976), Smith (1984), and Jones et al. (199I) have maintained isolates at room temperature."
Edited by sonoramo (12/18/20 03:42 PM)
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leroM
Registered: 11/13/17
Posts: 83
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Re: Some notes about LCs [Re: sonoramo]
#27096435 - 12/18/20 07:04 PM (3 years, 1 month ago) |
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Thanks for your contribution Blue, and the useful links.
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PitcherCrab
Crescent Fresh



Registered: 12/02/06
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Re: Some notes about LCs [Re: leroM]
#27098479 - 12/19/20 10:03 PM (3 years, 1 month ago) |
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The last two RW substrate bags I made I inoculated with 140ml of LC each. After over a week I didn’t have any mycelium growth in either, but one had a pretty big clump of spider mold going. Any ideas of what happened? I am kinda confused. My RW LC was super thick and the broth was crystal clear.
I just re-inoculated the one that didn’t take with a mystery variety (I think maybe Maz) and knocked up x2 new bags with GT and Corumba. Hoping for some better growth this round.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Veggiesandhemp
Strainger


Registered: 12/08/20
Posts: 77
Loc: Cascadia
Last seen: 1 year, 2 months
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Great thread thanks for the info. Looks alot more up my alley to get things rolling.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Sterile water storage is something I’ve been interested in for a while, too many refrigerator mishaps.
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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I did seven 20ml. slant tubes last week of lc. at different sterile water dilutions.
I went from strait lc. down to a 90% percent water added.
I drilled a hole in the lid just big enough for a 16 gauge needle.
After filling each tube I then hot glued the holes shut.
all tubes went into a quart jar then into the refrigerator.
I think they'll do just find if they're not refrigerated for a while as long as they're not expose to severe heat.
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Quote:
PitcherCrab said: The last two RW substrate bags I made I inoculated with 140ml of LC each. After over a week I didn’t have any mycelium growth in either, but one had a pretty big clump of spider mold going. Any ideas of what happened? I am kinda confused. My RW LC was super thick and the broth was crystal clear.
I just re-inoculated the one that didn’t take with a mystery variety (I think maybe Maz) and knocked up x2 new bags with GT and Corumba. Hoping for some better growth this round.
This is my bag after 12 days.
3 bags total. each bag is 6lb. of coir, verm. non prep. oats. 5 hours in the Presto. total moisture of sub. 65%.. 145ml. lc. added
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PitcherCrab
Crescent Fresh



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Re: Some notes about LCs [Re: micelio]
#27099166 - 12/20/20 11:12 AM (3 years, 1 month ago) |
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If they don't take this time I'm going to reduce the size of the bags.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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 It's getting there guys, I am too excited with how this is progressing.
Edited by mushhead (12/20/20 03:59 PM)
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Zifozonke
Stranger


Registered: 03/24/19
Posts: 1,258
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Re: Some notes about LCs [Re: mushhead] 2
#27099227 - 12/20/20 12:03 PM (3 years, 1 month ago) |
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Here are some test vials of some cultures I've got stored in sterile water What I did was place clean empty vials with caps on loose in a quart jar with SFD lid screwed on_Syringes were filled with distilled water wrapped in foil and the lot were PCed for 20 mins Once cool For each culture I squirted about 5mls onto a clean dish with healthy growth_ lightly scrapped the whole plate with the needle making sure not to go too deep into the agar _and then sucked the myceliated water into syringe and then back out into the sterilizefdglass vials which I then sealed with tape around the base of the cap and numbered which included a date and variety in a diary for future reference A fresh recycled syringe was used for each culture
 They were done in early July and still look fine Just stored in a cool cupboard Will get round to pulling some out next year sometime to see how they work Never done it before but I've got good faith that they'll wake up fine when out on some fresh agar again Seemed like a easy way to me to store cultures
All from this link https://www.shroomery.org/forums/showflat.php/Number/22554386
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CorteX_


Registered: 07/26/05
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Loc: The Hague, The Netherlands
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Re: Some notes about LCs [Re: Zifozonke]
#27099708 - 12/20/20 05:24 PM (3 years, 1 month ago) |
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Here's another reason why LC is amazing. Some time ago I did not have a pressure cooker. As such, I used plain brown rice to grow mushrooms. Although rice is great and easy to get, growth on grains is usually much faster. You have to wait for spores to germinate and when your jars are partially colonized you can't shake them because the rice is very clumpy. With LC though, you just inoculate 5-10cc per jar and shake the hell out of them right after. You'll see explosive growth in just a few days. I've had half pint rice jars colonize in as little as 10 days where it would take me about 3 weeks with just spores.
I advise everyone to give it a shot, and to minimize the risk of contamination people should always test their LC on agar or small jars with rice/grain.
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Celestialexplorer1


Registered: 03/25/20
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Re: Some notes about LCs [Re: CorteX_] 1
#27099938 - 12/20/20 08:24 PM (3 years, 1 month ago) |
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LC is the way, the truth and the light. Mostly when your a person who runs a lot of different species or varieties. And the simple fact your cutting down greatly on your contamination risk (as long as you follow simple sterile technique that is)
I’ll run species/strains in pint jars or all types and if they fruit good...
 ...save them in a vacationer. Or for species that take a long time to fruit and I want to save the culture from possibly being ruined due to ripped parafilm or something.
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 To spend just one moment in eternity
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Asura
Cyantist


Registered: 08/01/11
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Celestialexplorer1
You have a good understanding and outlook on lc's.
I would like to remind anyone that wants to store their lc's in vacutainers (blood collection tube) be sure to get BD red top.
I went to the local feed store last week to purchase some vacutainers for livestock and all they had was BD purple tops. The purple top contains sodium heparin as an anticoagulant. Not what you want for storing cultures.
Ebay:
https://www.ebay.com/sch/i.html?_from=R40&_trksid=m570.l1313&_nkw=brand+new+bd+vacutainer+10ml+redtop+tube+pack+of+100&_sacat=0&LH_TitleDesc=0&_odkw=brand+new+bd+vacutainer+10ml+redtop+tube+exp+06%2F22+%2B+pack+of+100&_osacat=0&_blrs=spell_check
Edited by micelio (12/20/20 09:45 PM)
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Asura
Cyantist


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Re: Some notes about LCs [Re: micelio]
#27100050 - 12/20/20 10:09 PM (3 years, 1 month ago) |
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I've been getting the BD vacutainers from Walmart Pet RX. Pretty good price.
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Lemgrub



Registered: 04/02/18
Posts: 589
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Re: Some notes about LCs [Re: Asura]
#27100075 - 12/20/20 10:33 PM (3 years, 1 month ago) |
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I honestly haven't gotten great results from LC so far. I'm still new to it and figuring out the nuances. However I've found that if I shake after inoculating the recovery is very poor, the growth rates for me haven't been very fast, and contamination can occur very easily if there's a lapse in technique.
I'm hoping I just don't have the best cultures yet and that I'm not achieving the correct LC density. I've had some success with it but it just hasn't been as fast as I thought it would be.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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A.k.a
Stranger



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Re: Some notes about LCs [Re: Lemgrub]
#27100239 - 12/21/20 03:01 AM (3 years, 1 month ago) |
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Shaking right after inoculation does seem to slow down the recovery. Letting it sit a few days until it gets a foothold and starts looking normal before shaking seems to be the best bet.
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LAGM2020     
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PitcherCrab
Crescent Fresh



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Re: Some notes about LCs [Re: A.k.a]
#27101652 - 12/21/20 08:32 PM (3 years, 1 month ago) |
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Quote:
A.k.a said: Shaking right after inoculation does seem to slow down the recovery. Letting it sit a few days until it gets a foothold and starts looking normal before shaking seems to be the best bet.
Ahhhh... this explains something I’ve been wondering about. I was under the impression I should be shaking directly after inoculation. I’m going to try letting it consolidate on my next batch.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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That's interesting because I've noticed the same thing when using liquid inoculation. I don't shake until it kicks off.
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
Edited by Nobler Hino (12/22/20 01:02 AM)
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A.k.a
Stranger



Registered: 10/27/19
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Yeah I shook the hell out of my jars knocked with lc up until recently. There wasn’t actually a huge difference in time between them cuz the shaken ones take longer to recover but when they did it was the whole jar at once pretty much.
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LAGM2020     
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