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Invisiblemicelio
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Re: Some notes about LCs [Re: PitcherCrab]
    #27078775 - 12/08/20 01:15 PM (3 years, 1 month ago)

Quote:

PitcherCrab said:
Blue Helix, I’ve got a question for you. When you make your substrate bags do you find the dry grain hydrates during your PC cycle and pulls some moisture out? I just made a couple 13.5lb bags consisting of hydrated coir, dry oats, wheat bran, vermiculite and water. They were just a tad wet going into the PC, but I’m hoping because I used dry grain it will hydrate during the cook and dry out the rest of the mix enough to accommodate the LC. I plan to inoculate them with ~140ml of LC.





I'm also doing the same.

Coir, oats and verm.

I made up three bags at 6 lbs. each.

Each bag got five hours in the Presto.

I used 140 ml. Lc for each bag.

I adjusted my moisture from 72% to 65% by adding verm and oats to the bags..

Keep us posted on your results. You should be seeing good growth by now..


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: micelio]
    #27079009 - 12/08/20 04:22 PM (3 years, 1 month ago)

Nothing yet... but my bags are in my garage which is ~56F. So things grow a bit more slowly in there. I'll keep you all posted with updates.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinebongoman
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Re: Some notes about LCs [Re: PitcherCrab]
    #27079230 - 12/08/20 06:32 PM (3 years, 1 month ago)

I grow my LC's in media bottles and pour to grain jars.

Is it too risky to aspirate LC directly from a media bottle to a sterilised syringe do you think?


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OfflineA.k.aM
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Re: Some notes about LCs [Re: bongoman]
    #27079445 - 12/08/20 08:28 PM (3 years, 1 month ago)

No that’s pretty common


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Offlinesonoramo
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Re: Some notes about LCs [Re: bongoman]
    #27080449 - 12/09/20 12:43 PM (3 years, 1 month ago)

Quote:

bongoman said:
I grow my LC's in media bottles and pour to grain jars.

Is it too risky to aspirate LC directly from a media bottle to a sterilised syringe do you think?




Not if you have an injection port on the media bottle. If you don't, maybe you could melt a small hole in the cap with a hot nail or something? To me, opening the media bottle and the grain jar seems much riskier than inoculating with a syringe. You inevitably draw ambient air into both the bottle and the jar as you pour, so that air had better not have dust or spores in it.

I really like long blunt dispensing needles. Mine are 16 gauge, 5 inches long.

One thing I really like about inoculating LC with a syringe is that I can sterilize the syringe without running my pressure canner. It takes 10 minutes to boil water and sterilize syringes ready for transfer. This is so much simpler than setting up the SAB or flow setup, wiping everything down and all the rest. So far, I've made dozens of transfers with no contamination.


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OfflineBrownBear
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Re: Some notes about LCs [Re: sonoramo]
    #27082796 - 12/10/20 06:12 PM (3 years, 1 month ago)

Will a healthy lc stay healthy for an extended period of time? I have about 8 lc's going right now of various varieties. Its definitely more than I need at this moment. I want to pull 1x12 cc syringe out of each of them every week. At this rate it would probably take me around 12 weeks (3 months) to pull them empty. Is this too long to leave them sitting around?



This lc was made on 11/20. I used 200 ml of water and around .5 grams of nutritional yeast. I was able to accomplish what I set out to accomplish. I have no doubt that I couldn't pull out even a 12 cc syringe of water without pulling mycelium with it. I wonder if I could lower the amount of water still without lowering the nutrition and have even less water in the final product but the same amount of mycelium?


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OfflineA.k.aM
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Re: Some notes about LCs [Re: BrownBear]
    #27083255 - 12/10/20 11:54 PM (3 years, 1 month ago)

They’ll last a while.

I recently found one a year old that had been sitting on a shelf in temps up to 110 for months during summer that still worked fine.


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OfflineBrownBear
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Re: Some notes about LCs [Re: A.k.a]
    #27083365 - 12/11/20 02:07 AM (3 years, 1 month ago)

That is great news. Thank you.


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: micelio]
    #27084556 - 12/11/20 06:48 PM (3 years, 1 month ago)

Quote:

micelio said:
Quote:

PitcherCrab said:
Blue Helix, I’ve got a question for you. When you make your substrate bags do you find the dry grain hydrates during your PC cycle and pulls some moisture out? I just made a couple 13.5lb bags consisting of hydrated coir, dry oats, wheat bran, vermiculite and water. They were just a tad wet going into the PC, but I’m hoping because I used dry grain it will hydrate during the cook and dry out the rest of the mix enough to accommodate the LC. I plan to inoculate them with ~140ml of LC.





I'm also doing the same.

Coir, oats and verm.

I made up three bags at 6 lbs. each.

Each bag got five hours in the Presto.

I used 140 ml. Lc for each bag.

I adjusted my moisture from 72% to 65% by adding verm and oats to the bags..

Keep us posted on your results. You should be seeing good growth by now..




Whelp... one of the RW bags appears to have cobweb mold. :nonono: Not what I was hoping for. I’m crossing my fingers the second bag isn’t the same but I haven’t seen any growth yet. I’m wondering if the cobweb (or whatever it is) made it into my LC somehow. Do any of you guys have experience with that? I know you can tell a bad LC if its cloudy from bacterial contamination but not so sure if you can do the same if its contaminated with mold.

Regardless of what happens I’m going to make some more bags and knock them up with one of my other LCs. My GT clone seems to be pinning under the liquid in its LC so I may try that one next.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



Edited by PitcherCrab (12/11/20 06:57 PM)


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Invisiblemicelio
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Re: Some notes about LCs [Re: PitcherCrab]
    #27084750 - 12/11/20 09:10 PM (3 years, 1 month ago)

If I look hard I can see a few little white spots showing up now on all three bags.

From what I notice with mold In my lc. Is It normally floats to the surface looking like white mycelium spots and turning green in a day or two.

Cobweb mold could act different though..

I don't use my cheap ebay impulse sealer anymore. I don't trust the seal. I tie the top close with twine or plastic tie wraps.


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: micelio]
    #27084872 - 12/12/20 12:12 AM (3 years, 1 month ago)

Quote:

micelio said:
If I look hard I can see a few little white spots showing up now on all three bags.

From what I notice with mold In my lc. Is It normally floats to the surface looking like white mycelium spots and turning green in a day or two.

Cobweb mold could act different though..

I don't use my cheap ebay impulse sealer anymore. I don't trust the seal. I tie the top close with twine or plastic tie wraps.




Yeah, that’s been my experience with mold too – it floats to the top of the LC. Not sure what the deal with cobweb is... I had this happen once before where I tried to inoculate a bunch of bags only to have grown a bunch of cobweb. I was sure my LC was clean, but who knows. I think I’m going to make some PF cakes or something to test my LC just to see if that was the issue. I saved some of it in vacutainers so I can start some larger batches from that.

I feel you on the impulse sealer. I have one but it never really seals quite as well as I want it to, so now I just use Monoprice reusable zip ties on all my bags.

I’m a bit frustrated with the cobweb situation but I’m going to get it all straightened out. I’d really like to get this process down.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineFailboat
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Re: Some notes about LCs [Re: PitcherCrab]
    #27084887 - 12/12/20 12:25 AM (3 years, 1 month ago)

I've got some clean looking transfers from one of my Melmac clone cultures. Gonna make like 4 LC, 2 plates into 2 each. I've got malt, but I thought maybe molasses? Hoping for excellent results after a few bottles lost to learning curves. Plus now I've my FFU.


Edited by Failboat (12/12/20 12:27 AM)


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Invisiblemicelio
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Re: Some notes about LCs [Re: Failboat]
    #27085536 - 12/12/20 11:20 AM (3 years, 1 month ago)

This Is 4% broth.

1000ml. ro. water.
20 grams malt extract.
20 grams dextrose (corn sugar)

7 salsa jars.
140ml. broth per jar.
each jar received 5ml. liquid culture.



7 days growth:



12 days growth:



I been playing with lc's for seven years and this is the fastest growing lc I've had.

This is the most comprehensive write up on liquid culture to date on this site that I know of.

My Thanks to Blue Helix for sharing his notes...


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OfflineAsuraS
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Re: Some notes about LCs [Re: micelio]
    #27085636 - 12/12/20 12:15 PM (3 years, 1 month ago)

I just did some bags with BH's 4% and the growth was insane. Bags colonized really fast
and when I put them to trays it was fully recovered in 3 days instead of the usual
5. My jars were ugly as fuck though. Could hardly see shit bc of the sediment but mine
grew out in 4 days.


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InvisiblePsilosion

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Re: Some notes about LCs [Re: Asura]
    #27087236 - 12/13/20 12:57 PM (3 years, 1 month ago)

Just made two PEU liquid cultures today with two different T3's I had. Used about .2% LME and .1% agar just to see what happens.

I read somewhere in this thread or maybe another; someone putting in a small amount of agar in their LC's and I wanted to try it.


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Invisiblemicelio
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Re: Some notes about LCs [Re: Psilosion]
    #27094575 - 12/17/20 05:13 PM (3 years, 1 month ago)

18 days growth....

This Is 4% broth.

1000ml. ro. water.
20 grams malt extract.
20 grams dextrose (corn sugar)

7 salsa jars.
140ml. broth per jar.
each jar received 5ml. liquid culture.



7 days growth:



12 days growth:



18 days growth, It's getting thick.. Not much light getting through:



The LC been on a slow spin, approx. 200 rpm..


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OfflineA.k.aM
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Re: Some notes about LCs [Re: micelio]
    #27094975 - 12/17/20 09:21 PM (3 years, 1 month ago)

Damn that’s thick!

I gotta try some nute heavy broth next time, mine always stop growing after a while I never see crazy thick myc or where people get it fruiting on top.


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Invisiblemicelio
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Re: Some notes about LCs [Re: A.k.a]
    #27095547 - 12/18/20 09:37 AM (3 years, 1 month ago)

If you can take some pic's of your lc progress as It grows out.
It would be nice to get some comparisons.

Also my opinion is, Making smaller batches under 150ml. gives for faster healthy growth.


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Offlinesonoramo
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Re: Some notes about LCs [Re: A.k.a]
    #27095807 - 12/18/20 12:53 PM (3 years, 1 month ago)

Quote:

A.k.a said:
Damn that’s thick!

I gotta try some nute heavy broth next time, mine always stop growing after a while I never see crazy thick myc or where people get it fruiting on top.




Is that really what we want, though? I'm not a full-time grower, so the ability I want is to conveniently prepare for a grow every few months. I'm more interested in keeping a viable culture available than just growing as many pounds per week as possible.

I have a bunch of LC's and some are now more than a month old. They are pretty thick and I'm ready to toss them because it will be so hard to draw the mycelium into a syringe. What I'd really like is to have a small number of LC cultures (maybe in the refrigerator). Also have some un-inoculated LC jars on the shelf. I imagine starting a grow by inoculating one or two LCs from the cultures, then grain jars from that a week later.

I guess what I'm asking is: Are heavy nutrients in a broth helpful for a culture that's intended for storage?


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Invisiblemicelio
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Re: Some notes about LCs [Re: sonoramo]
    #27095858 - 12/18/20 01:39 PM (3 years, 1 month ago)

Blue Helix explains this issue in this thread..

https://www.shroomery.org/forums/showflat.php/Number/26997136#26997136


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